Lactobacillus johnsonii alleviates colitis by TLR1/2-STAT3 mediated CD206 ⁺ macrophages ^IL-10 activation

Abstract

Imbalance of gut microbiota homeostasis is related to the occurrence of ulcerative colitis (UC), and probiotics are thought to modulate immune microenvironment and repair barrier function. Here, in order to reveal the interaction between UC and gut microbiota, we screened a new probiotic strain by 16S rRNA sequencing from Dextran Sulfate Sodium (DSS)-induced colitis mice, and explored the mechanism and clinical relevance. Lactobacillus johnsonii ( L. johnsonii ), as a potential anti-inflammatory bacterium was decreased colonization in colitis mice. Gavage L. johnsonii could alleviate colitis by specifically increasing the proportion of intestinal macrophages and the secretion of Il-10 with macrophages depleted model and in Il10 −/− mice. We identified this subset of immune cells activated by L. johnsonii as CD206 + macrophages IL−10 . Mechanistically, L. johnsonii supplementation enhanced the mobilization of CD206 + macrophages IL−10 through the activation of STAT3 in vivo and in vitro . In addition, we revealed that TLR1/2 was essential for the activation of STAT3 and the recognition of L. johnsonii by macrophages. Clinically, there was positive correlation between the abundance of L. johnsonii and the expression level of MRC1, IL10 and TLR1/2 in UC tissues. L. johnsonii could activate native macrophages into CD206 + macrophages and release IL-10 through TLR1/2-STAT3 pathway to relieve experimental colitis. L. johnsonii may serve as an immunomodulator and anti-inflammatory therapeutic target for UC.

Cholesterol lowering in diet-induced hypercholesterolemic mice using Lactobacillus bile salt hydrolases with different substrate specificities

“…Notably, L. reuteri and L. johnsonii have been widely reported in various studies for their probiotic effects. 28–30 These findings demonstrate that recombinant BSH derived from YB81 can ameliorate dysbiosis induced by HCD, which may be linked to its CHOL-lowering capabilities.…”

Propionic Acid Driven by the Lactobacillus johnsonii Culture Supernatant Alleviates Colitis by Inhibiting M1 Macrophage Polarization by Modulating the MAPK Pathway in Mice

“…17 As a probiotic, Lactobacillus johnsonii is well-known for its ability to enhance the host's immune response and has significantly alleviated colitis in the mouse model. 18 L. johnsonii supplementation reduces colitis by modulating inflammation and endoplasmic reticulum stress. 18,19 However, different strains of the same genus of bacteria have functional specificity and may exert different effects on disease.…”

“…As a probiotic, Lactobacillus johnsonii is well-known for its ability to enhance the host’s immune response and has significantly alleviated colitis in the mouse model , However, different strains of the same genus of bacteria have functional specificity and may exert different effects on disease. Previously, different strains of B. longum and Akkermansia muciniphila were shown to inhibit colitis in mice in a strain-specific manner. , In addition, two Lactobacillus murinus strains showed different anti-inflammatory properties in a Caco-2 cell model .…”

“…18 L. johnsonii supplementation reduces colitis by modulating inflammation and endoplasmic reticulum stress. 18,19 However, different strains of the same genus of bacteria have functional specificity and may exert different effects on disease. Previously, different strains of B. longum and Akkermansia muciniphila were shown to inhibit colitis in mice in a strain-specific manner.…”

“…Tryptophan and its metabolites can improve intestinal microbiota dysbiosis and enhance intestinal barrier function. , The metabolites of bile acids have also been shown to alleviate colonic inflammation . As a probiotic, Lactobacillus johnsonii is well-known for its ability to enhance the host’s immune response and has significantly alleviated colitis in the mouse model , However, different strains of the same genus of bacteria have functional specificity and may exert different effects on disease.…”