Lactobacillus johnsoniiLa1 AntagonizesGiardia intestinalisIn Vivo

Abstract

This study describes the in vivo activity of Lactobacillus johnsonii La1 (NCC533) in Giardia intestinalisinfected gerbils (Meriones unguiculatus). Daily administration of lactobacilli in the drinking water from 7 days before inoculation with Giardia trophozoites efficiently prevented G. intestinalis strain WB clone C6 from infecting gerbils. More specifically, shedding of fecal Giardia antigens (GSA65 protein) was diminished in the La1-treated group, and resolution of infection was observed by 21 days postinoculation. Histology and analysis of enzymatic markers of microvillus membrane integrity revealed that probiotic administration also protected against parasite-induced mucosal damage. In addition, a cellular response to Giardia antigens was stimulated in spleen cells from La1-treated gerbils. Results show for the first time the antigiardial effect of probiotic lactobacilli in vivo and provide further insight into the antagonistic properties of lactic acid bacteria against protozoa involved in intestinal infections.

Modern Aspects of Giardiasis Diagnosis and Treatment

“…В ряде исследований in vitro и in vivo продемон-стрирован прямой антагонистический эффект штамма Lactobacillus johnsonii La1 на трофозоиды лямблий [21], а также иммуноопосредованный противолямблиозный эффект данного штамма за счет стимуляции гумораль-ных и клеточных иммунных реакций на инвазию [22].…”

Potencial bioterapêutico dos probióticos nas parasitoses intestinais

“…Nos ensaios in vivo, a administração prévia de L. johnsonii La1 a gerbilíneos (Meriones unguiculatus) também se revelou capaz de reduzir a taxa de infecção dos animais e a excreção de antígenos de Giardia nas fezes (HUMEN et al, 2005). Outra evidência da proteção conferida pelo probiótico foi a preservação da integridade histológica da mucosa intestinal e de marcadores enzimáticos no intestino dos animais tratados.…”

“…Atualmente, as drogas disponíveis para o tratamento da giardíase incluem derivados de nitroimidazóis e nitrofuranos, tais como metronidazol, tinidazol, furazolidona e albendazol (HUMEN et al, 2005). No entanto, de acordo com WRIGHT et al (2003), os registros clínicos e laboratoriais de resistência contra essas drogas reforçam a necessidade de se identificar novos agentes contra a giardíase e alternativas que preservem a viabilidade das drogas existentes.…”

In vitro and in vivo effects of Enterococcus faecalis CECT7121 on Toxocara canis

“…Whereas probiotics have been used successfully for the treatment of several intestinal disorders (Heyman 2000, de Vrese & Schrezenmeir 2008, they have not yet been applied for the control of human intestinal parasitoses. However, they have been used successfully in animals for the treatment of parasitoses, such as cryptosporidiosis, giardiasis, trichinellosis and babesiosis (Alak et al 1999, Bautista-Garfias et al 2001, Humen et al 2005. In contrast, Dea-Ayuela et al (2008) (Sparo et al 2006).…”