<i>Lactobacillus plantarum </i><scp>BS</scp>22 promotes gut microbial homeostasis in broiler chickens exposed to aflatoxin B<sub>1</sub>

Zeng, Yan; Zeng, Dong; Zhang, Y.; Wang, J.; Jian, Ping; Zhou, Yi; Li, Y.; Yin, Zhongqiong; Pan, Kangcheng; Jing, Bo

doi:10.1111/jpn.12766

Cited by 19 publications

(10 citation statements)

References 48 publications

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“…These findings indicate that the microbial colonization of the gastrointestinal tract gradually changes until a mature and complex microbial community is established ( Babot et al., 2014 ). Several other studies have demonstrated that Lactobacillus could be potential to prevent the colonization of pathogens, promote intestinal health, and improve growth ( Jones and Versalovic, 2009 ; Zeng et al., 2017 ). The Spearman analyses further showed that the Lactobacillus was positively correlated with the VH and VH:CD in the ileum of the growing broilers.…”

Section: Discussionmentioning

confidence: 99%

The relationship among gut microbiota, short-chain fatty acids, and intestinal morphology of growing and healthy broilers

Liao¹,

Shao²,

Sun³

et al. 2020

Poultry Science

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The gut microbiota play an important role in the growth and intestinal health of broilers. The present study was to investigate the gut microbiota, short-chain fatty acids, and intestinal morphology of broilers at different ages. A total of 320 one-day-old male broilers were raised in 8 replicates and fed the same corn–soybean diets for 42 D. The duodenal, jejunal, and ileal segments and their and cecal microbiota were collected on day 1, 7, 14, 21, and 42, respectively. The villous height ( VH ), crypt depth ( CD ), and their ratio of VH:CD in the duodenum, jejunum, and ileum all increased ( P < 0.05) with age. Caecal acetate, propionate, butyrate, valerate, and isovalerate increased ( P < 0.01), but isobutyrate decreased ( P < 0.001) with age. The cecum had the greatest ( P < 0.001) alpha diversity of bacterial community in broilers at different ages. Beta diversities showed distinct differences in gut microbial compositions among different ages (R = 0.55, P < 0.002) and different intestinal segments (R = 0.53, P < 0.002). Lactobacillus was the most abundant genus in the duodenum (36∼97%), jejunum (39∼72%), and ileum (24∼96%) at all ages, and in the ileum, it was positively correlated with VH (R = 0.559, P < 0.03), VH:CD (R = 0.55, P < 0.03), and acetate contents (R = 0.541, P < 0.04) but negatively correlated (R = -0.50, P < 0.05) with isobutyrate contents. Escherichia–Shigella and Salmonella dominated in the cecum of newly hatched broilers, and then the Bacteroides dominated in the cecum on day 42. In the cecum, Escherichia–Shigella was positively correlated (R = 0.577∼0.662, P < 0.05) with isobutyrate contents and Salmonella negatively correlated (R = -0.539∼-0.843, P < 0.05) with isovalerate, butyrate, and acetate contents. These aforementioned results indicated that the most abundant Lactobacillus from the small intestine and the most diversity of microflora community and short-chain fatty acids in the cecum might contribute to the development of intestinal structure in the whole growing period of broilers.

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Section: Discussionmentioning

confidence: 99%

The relationship among gut microbiota, short-chain fatty acids, and intestinal morphology of growing and healthy broilers

Liao¹,

Shao²,

Sun³

et al. 2020

Poultry Science

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“…L. plantarum strains from different food sources have been reported for their ability to control A. flavus and aflatoxins. L. plantarum strains possess the ability to reduce the toxicity of aflatoxins through binding activities or promoting gut microbial homeostasis in broiler chickens exposed to aflatoxin B 1 [21,22,23]. In our current study, strain IAMU80070 was isolated from kimchi and characterized.…”

Section: Discussionmentioning

confidence: 99%

Morphological and Transcriptomic Analysis of the Inhibitory Effects of Lactobacillus plantarum on Aspergillus flavus Growth and Aflatoxin Production

Zhao

Zhang

Folly

et al. 2019

Toxins

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Lactobacillus plantarum, as a natural bio-preservative, has attracted a great deal of attention in recent years. In this study, 22 L. plantarum strains were tested against the aflatoxin-producing fungus, Aspergillus flavus; strain IAMU80070 showed the highest antifungal activity. At a concentration of 5 × 105 colony-forming units (CFU) mL−1, it completely inhibited A. flavus growth and decreased aflatoxin production by 93%. Furthermore, ultrastructural examination showed that IAMU80070 destroyed the cellular structure of hyphae and spores. To explore the inhibitory effect of IAMU80070 on A. flavus at the transcriptional level, transcriptome data were obtained and subjected to Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses. The aflatoxin biosynthetic process was the most significantly downregulated functional category, while genes implicated in the synthesis and organization of cell wall polysaccharides were upregulated. Quantitative real-time PCR results verified the credibility and reliability of the RNA sequencing data. These results provided insight into the transcriptome of A. flavus in response to the antagonistic effects of L. plantarum IAMU80070.

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“…The perinatal and neonatal GIT is more sensitive to bacteria, as these intestinal cells have increased plasticity to reprogram the transcriptional and proteomic profile, which may predispose the host to disease or improved performance into adult life ( Cortese et al., 2016 ). Knowledge about the role of commensal Enterobacteriaceae found in chicks is generally limited, beyond their role as pathogens ( Crhanova et al., 2011 , Awad et al., 2016 , Zeng et al., 2018 ). The in ovo technique applied here may allow for the control of pioneer colonization and to influence the GIT development based on the pioneering microbiota in the host.…”

Section: Discussionmentioning

confidence: 99%

Impact of in ovo administered pioneer colonizers on intestinal proteome on day of hatch

et al. 2020

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Pioneer colonization of the gastrointestinal tract ( GIT ) by bacteria is thought to have major influence on neonatal tissue development. Previous studies have shown in ovo inoculation of embryos with saline (S), species of Citrobacter (C, C2), or lactic acid bacteria (L) resulted in an altered microbiome on day of the hatch ( DOH ). The present study investigated GIT proteomic changes at DOH in relation to different inoculations. Embryos were inoculated in ovo with S or ∼10 2 cfu of C, C2, or L at 18 embryonic days. On DOH, the GIT was collected, and tissue proteins were extracted for analysis via tandem mass spectrometry. A total of 493 proteins were identified for differential comparison with S at P ≤ 0.10. Different levels were noted in 107, 39, and 78 proteins in C, C2, and L groups, respectively, which were uploaded to Ingenuity Pathway Analysis to determine canonical pathways and biological functions related to these changes. Three members of the cytokine family (interleukin [ IL ]-1β, IL6, and Oncostatin M) were predicted to be activated in C2, indicated with Z-score ≥ 1.50, which suggested an overall proinflammatory GIT condition. This was consistent with the activation of the acute-phase response signaling pathway seen exclusively in C2 (Z-score = 2.00, P < 0.01). However, activation (Z-score = 2.00) of IL-13, upregulation of peroxiredoxin-1 and superoxide dismutase 1, in addition to activation of nitric oxide signaling in the cardiovascular system of the L treatment may predict a state of increased antioxidant capacity and decreased inflammatory status. The nuclear factor erythroid 2-related factor 2 ( NRF2 )-mediated oxidative stress response (Z-score = 2.00, P < 0.01) was predicted to be upregulated in C which suggested that chicks were in an inflammatory state and associated oxidative stress, but the impact of these pathways differed from that of C2. These changes in the proteome suggest that pioneer colonizing microbiota may have a strong impact on pathways associated with GIT immune and cellular development.

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Lactobacillus plantarum BS22 promotes gut microbial homeostasis in broiler chickens exposed to aflatoxin B₁

Cited by 19 publications

References 48 publications

The relationship among gut microbiota, short-chain fatty acids, and intestinal morphology of growing and healthy broilers

The relationship among gut microbiota, short-chain fatty acids, and intestinal morphology of growing and healthy broilers

Morphological and Transcriptomic Analysis of the Inhibitory Effects of Lactobacillus plantarum on Aspergillus flavus Growth and Aflatoxin Production

Impact of in ovo administered pioneer colonizers on intestinal proteome on day of hatch

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Lactobacillus plantarum BS22 promotes gut microbial homeostasis in broiler chickens exposed to aflatoxin B1

Cited by 19 publications

References 48 publications

The relationship among gut microbiota, short-chain fatty acids, and intestinal morphology of growing and healthy broilers

The relationship among gut microbiota, short-chain fatty acids, and intestinal morphology of growing and healthy broilers

Morphological and Transcriptomic Analysis of the Inhibitory Effects of Lactobacillus plantarum on Aspergillus flavus Growth and Aflatoxin Production

Impact of in ovo administered pioneer colonizers on intestinal proteome on day of hatch

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Lactobacillus plantarum BS22 promotes gut microbial homeostasis in broiler chickens exposed to aflatoxin B₁