<i>Lactobacillus plantarum</i> supplementation alleviates liver and intestinal injury in parenteral nutrition‐fed piglets

Wang, Weipeng; Wang, Ying; Liu, Yang; Tian, Xinbei; Chen, Shanshan; Lu, Ying; Wu, Bo; Xiao, Yongtao; Cai, Wei

doi:10.1002/jpen.2429

Cited by 3 publications

(1 citation statement)

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“…In this study, we found that all the indicated SCFAs decreased significantly in the TPN group. These findings were rational because one of our previous works has demonstrated that the abundance of SCFA-producing Lactobacillus decreased dramatically in the ileal content of TPN piglets [27]. In children, studies using fecal samples have also demonstrated that the abundance of SCFAs (butanoic and pentanoic acids) in SBS patients weaning on PN was significantly lower than their counterparts weaning of PN [28,29], concomitant with reduced abundance of SCFA-producing bacteria such as Lachnospiraceae and Ruminococcaceae.…”

Section: Discussionmentioning

confidence: 74%

Multi-Omics Unravels Metabolic Alterations in the Ileal Mucosa of Neonatal Piglets Receiving Total Parenteral Nutrition

et al. 2023

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Total parenteral nutrition (TPN) is life-saving therapy for the pediatric patients with intestinal failure (IF) who cannot tolerate enteral nutrition (EN). However, TPN-induced metabolic alterations are also a critical issue for the maintenance of intestinal homeostasis, and thus the global metabolomic signatures need to be addressed. In this study, ileal mucosal biopsies were collected from 12 neonatal Bama piglets receiving either EN or TPN for 14 days, and changes in the intestinal metabolism were examined by multi-omics (HM350 Metabolomics + Tandem Mass Tag (TMT)-based proteomics). As a result, a total of 240 compounds were identified by metabolomics, including 56 down-regulated and 9 up-regulated metabolites. Notably, tissue levels of fatty acyl-carnitines (decreased by 35–85%) and succinate (decreased by 89%) dramatically decreased in the TPN group, suggestive of disrupted processes of fatty acid oxidation (FAO) and the citrate cycle, respectively. Interestingly, however, no differences were found in the production of adenosine 5′-triphosphate (ATP) between groups, suggesting that these dysregulated metabolites may have mainly led to the loss of bioactive compounds rather than energy deficit. Additionally, 4813 proteins were identified by proteomics in total, including 179 down-regulated and 329 up-regulated proteins. The analysis of protein–protein interactions (PPI) indicated that most of the differentially expressed proteins were clustered into “lipid metabolism” and “innate immune responses”. In summary, this work provided new findings in TPN-induced intestinal metabolic alterations, which would be useful to the improvement of nutritional management for IF patients.

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Section: Discussionmentioning

confidence: 74%

Multi-Omics Unravels Metabolic Alterations in the Ileal Mucosa of Neonatal Piglets Receiving Total Parenteral Nutrition

et al. 2023

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Research Progress on the Role of Gut Microbiota and Its Metabolites in the Occurrence and Development of Septic-Associated Liver Injury

Chen,

Song,

Zeng

et al. 2024

Shock

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Sepsis is a life-threatening organ dysfunction that occurs due to a dysregulated host response to infection. Septic-associated liver injury (SALI) has been closely linked to the prognosis and mortality of sepsis. Recent investigations have delved into the gut-liver axis and its association with SALI, identifying its pivotal role in the gut microbiota. Bacterial translocation and the onset of SALI can occur due to an imbalance in the gut microbiota, impairing the function of the gut barrier. Moreover, their metabolites might exacerbate or initiate SALI by modulating immune responses. Nevertheless, interventions to restore the balance of the gut microbiota, such as the administration of probiotics, fecal microbiota transplantation, or dietary adjustments, may ameliorate SALI and enhance the prognosis and survival rates of septic patients. This review aimed to elucidate the function of the gut microbiota in the genesis and procession of SALI and its potential therapeutic value, offering a deeper understanding of the pathogenesis and therapeutic avenues for SALI.

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Altered hepatic and intestinal homeostasis in a neonatal murine model of short-term total parenteral nutrition and antibiotics

Mims,

Kumari,

Leathem

et al. 2023

American Journal of Physiology-Gastrointestinal and Liver Physi

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Parenteral nutrition (PN) prevents starvation and supports metabolic requirements intravenously when patients are unable to be fed enterally. Clinically, infants are frequently provided PN in intensive care settings along with exposure to antibiotics (ABX) to minimize infection during care. Unfortunately, neonates experience extremely high rates of hepatic complications Adult rodent and piglet models of PN are well-established, but neonatal models capable of leveraging the considerable transgenic potential of the mouse remain underdeveloped. Utilizing our newly established neonatal murine PN mouse model, we administered ABX or controlled drinking water to timed pregnant dams to disrupt the maternal microbiome. We randomized mouse pups to PN or sham surgery controls +/- ABX exposure. ABX or short-term PN decreased liver and brain organ weights, intestinal length, and mucosal architecture (Vs. controls). PN significantly elevated evidence of hepatic proinflammatory markers, neutrophils and macrophage counts, bacterial colony forming units, and evidence of cholestasis risk, which was blocked by ABX. However, ABX uniquely elevated metabolic regulatory genes resulting in accumulation of hepatocyte lipids, triglycerides, and elevated TCDCA in serum. Within the gut, PN elevated the relative abundance of Akkermansia, Enterococcus, and Suterella with decreased Anaerostipes and Lactobacillus compared with controls, while ABX enriched Proteobacteria. We conclude that short-term PN elevates hepatic inflammatory stress and risk of cholestasis in early life. While concurrent ABX exposure protects against hepatic immune activation during PN, the dual exposure modulates metabolism and may contribute towards early steatosis phenotype, sometimes observed in infants unable to wean from PN.

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Lactobacillus plantarum supplementation alleviates liver and intestinal injury in parenteral nutrition‐fed piglets

Cited by 3 publications

References 34 publications

Multi-Omics Unravels Metabolic Alterations in the Ileal Mucosa of Neonatal Piglets Receiving Total Parenteral Nutrition

Multi-Omics Unravels Metabolic Alterations in the Ileal Mucosa of Neonatal Piglets Receiving Total Parenteral Nutrition

Research Progress on the Role of Gut Microbiota and Its Metabolites in the Occurrence and Development of Septic-Associated Liver Injury

Altered hepatic and intestinal homeostasis in a neonatal murine model of short-term total parenteral nutrition and antibiotics

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