<i>Lactobacillus rhamnosus</i> GG administration partially prevents diet-induced insulin resistance in rats: a comparison with its heat-inactivated parabiotic

Arellano-García, L.; Macarulla, M. T.; Cuevas-Sierra, A.; Martínez, J. A.; Portillo, M. P.; Milton-Laskibar, I.

doi:10.1039/d3fo01307c

Cited by 9 publications

(2 citation statements)

References 60 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…61,62 Our findings with Lactobacillus rhamnosus SG069 (LR069) and Lactobacillus brevis SG031 (LB031) complement this body of knowledge, showing significant metabolic improvements in mice on a high-fat diet (HFD). Similar to the study on Lactobacillus rhamnosus GG, which highlighted the strain's ability to prevent dietinduced insulin resistance, 63 LR069 and LB031 demonstrated substantial reductions in body weight, adipose tissue mass, and liver weight, showcasing their potential in countering obesity's adverse effects.…”

Section: Discussionmentioning

confidence: 60%

Lactobacillus rhamnosus 069 and Lactobacillus brevis 031: Unraveling Strain-Specific Pathways for Modulating Lipid Metabolism and Attenuating High-Fat-Diet-Induced Obesity in Mice

Ho,

Chou,

Koh

et al. 2024

ACS Omega

View full text Add to dashboard Cite

Obesity is a global health crisis, marked by excessive fat in tissues that function as immune organs, linked to microbiota dysregulation and adipose inflammation. Investigating the effects of Lactobacillus rhamnosus SG069 (LR069) and Lactobacillus brevis SG031 (LB031) on obesity and lipid metabolism, this research highlights adipose tissue's critical immune-metabolic role and the probiotics' potential against diet-induced obesity. Mice fed a high-fat diet were treated with either LR069 or LB031 for 12 weeks. Administration of LB031 boosted lipid metabolism, indicated by higher AMP-activated protein kinase (AMPK) and acetyl-CoA carboxylase (ACC) phosphorylation, and increased the M2/M1 macrophage ratio, indicating LB031's antiinflammatory effect. Meanwhile, LR069 administration not only led to significant weight loss by enhancing lipolysis which evidenced by increased phosphorylation of hormonesensitive lipase (HSL) and adipose triglyceride lipase (ATGL) but also elevated Akkermansia and fecal acetic acid levels, showing the gut microbiota's pivotal role in its antiobesity effects. LR069 and LB031 exhibit distinct effects on lipid metabolism and obesity, underscoring their potential for precise interventions. This research elucidates the unique impacts of these strains on metabolic health and highlights the intricate relationship between gut microbiota and obesity, advancing our knowledge of probiotics' therapeutic potential.

show abstract

Section: Discussionmentioning

confidence: 60%

Lactobacillus rhamnosus 069 and Lactobacillus brevis 031: Unraveling Strain-Specific Pathways for Modulating Lipid Metabolism and Attenuating High-Fat-Diet-Induced Obesity in Mice

Ho,

Chou,

Koh

et al. 2024

ACS Omega

View full text Add to dashboard Cite

show abstract

“…Lactobacillus gasseri PA3 can decrease the UA concentration and increase the relative abundance of Lactobacillus 23 , 24 . Lactobacillus rhamnosus GG (LGG), a gastrointestinal probiotic strain 25 , has been reported to have beneficial effects with regard to metabolic diseases such as diabetes 26 , hypertension 27 , insulin resistance 28 , and obesity 29 . Studies have shown that LGG ameliorates metabolic disorders in the body mainly by affecting in the intestinal microbiota 30 , such as decreasing opportunistic bacteria ( Bacteroidetes and Proteobacteria ) and increasing beneficial bacteria ( Lactobacillus , Bifidobacterium , Butyricicoccus ) 31 – 33 .…”

Section: Introductionmentioning

confidence: 99%

Lactobacillus rhamnosus GG ameliorates hyperuricemia in a novel model

Fu,

Chen,

Xia

et al. 2024

npj Biofilms Microbiomes

View full text Add to dashboard Cite

Hyperuricemia (HUA) is a metabolic syndrome caused by abnormal purine metabolism. Although recent studies have noted a relationship between the gut microbiota and gout, whether the microbiota could ameliorate HUA-associated systemic purine metabolism remains unclear. In this study, we constructed a novel model of HUA in geese and investigated the mechanism by which Lactobacillus rhamnosus GG (LGG) could have beneficial effects on HUA. The administration of antibiotics and fecal microbiota transplantation (FMT) experiments were used in this HUA goose model. The effects of LGG and its metabolites on HUA were evaluated in vivo and in vitro. Heterogeneous expression and gene knockout of LGG revealed the mechanism of LGG. Multi-omics analysis revealed that the Lactobacillus genus is associated with changes in purine metabolism in HUA. This study showed that LGG and its metabolites could alleviate HUA through the gut-liver-kidney axis. Whole-genome analysis, heterogeneous expression, and gene knockout of LGG enzymes ABC-type multidrug transport system (ABCT), inosine-uridine nucleoside N-ribohydrolase (iunH), and xanthine permease (pbuX) demonstrated the function of nucleoside degradation in LGG. Multi-omics and a correlation analysis in HUA patients and this goose model revealed that a serum proline deficiency, as well as changes in Collinsella and Lactobacillus, may be associated with the occurrence of HUA. Our findings demonstrated the potential of a goose model of diet-induced HUA, and LGG and proline could be promising therapies for HUA.

show abstract

Comparative effects of viable Lactobacillus rhamnosus GG and its heat‐inactivated paraprobiotic in the prevention of high‐fat high‐fructose diet‐induced non‐alcoholic fatty liver disease in rats

Arellano‐García,

Milton‐Laskibar,

Martínez

et al. 2024

BioFactors

View full text Add to dashboard Cite

Nonalcoholic fatty liver disease (NAFLD) is one of the most prevalent chronic liver alterations worldwide, being gut microbiota dysbiosis one of the contributing factors to its development. The aim of this research is to compare the potential effects of a viable probiotic (Lactobacillus rhamnosus GG) with those exerted by its heat‐inactivated paraprobiotic counterpart in a dietary rodent model of NAFLD. The probiotic administration effectively prevented the hepatic lipid accumulation induced by a high‐fat high‐fructose diet feeding, as demonstrated by chemical (lower TG content) and histological (lower steatosis grade and lobular inflammation) analyses. This effect was mainly mediated by the downregulation of lipid uptake (FATP2 protein expression) and upregulating liver TG release to bloodstream (MTTP activity) in rats receiving the probiotic. By contrast, the effect of the paraprobiotic preventing diet‐induced liver lipid accumulation was milder, and mainly derived from the downregulation of hepatic de novo lipogenesis (SREBP‐1c protein expression and FAS activity) and TG assembly (DGAT2 and AQP9 protein expression). The obtained results demonstrate that under these experimental conditions, the effects induced by the administration of viable L. rhamnosus GG preventing liver lipid accumulation in rats fed a diet rich in saturated fat and fructose differ from those induced by its heat‐inactivated paraprobiotic counterpart.

show abstract

Lactobacillus rhamnosus GG administration partially prevents diet-induced insulin resistance in rats: a comparison with its heat-inactivated parabiotic

Abstract: Insulin resistance and type 2 diabetes are obesity-related health alterations featuring an ever increasing prevalence. Besides inadequate feeding patterns, gut microbiota alterations stand out as potential contributors of these metabolic...

Cited by 9 publications

References 60 publications

Lactobacillus rhamnosus 069 and Lactobacillus brevis 031: Unraveling Strain-Specific Pathways for Modulating Lipid Metabolism and Attenuating High-Fat-Diet-Induced Obesity in Mice

Lactobacillus rhamnosus 069 and Lactobacillus brevis 031: Unraveling Strain-Specific Pathways for Modulating Lipid Metabolism and Attenuating High-Fat-Diet-Induced Obesity in Mice

Lactobacillus rhamnosus GG ameliorates hyperuricemia in a novel model

Comparative effects of viable Lactobacillus rhamnosus GG and its heat‐inactivated paraprobiotic in the prevention of high‐fat high‐fructose diet‐induced non‐alcoholic fatty liver disease in rats

Contact Info

Product

Resources

About