<i>Lactobacillus rhamnosus</i> GG ameliorates deoxynivalenol-induced kidney oxidative damage and mitochondrial injury in weaned piglets

Ma, Kaidi; Bai, Yongsong; Li, Jibo; Ren, Zhongshuai; Li, Jianping; Zhang, Jing; Shan, Anshan

doi:10.1039/d2fo00185c

Cited by 33 publications

(28 citation statements)

References 67 publications

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“…In this study, a total antioxidant capacity assay to characterize DE111 ® activity was used. In this assay L. rhamnosus GG was used as a comparator strain as its antioxidant capacity has been previously reported (Ma et al, 2022). While, both DE111 ® and L. rhamnosus GG demonstrated antioxidant activity when measured by this assay, the levels varied significantly between the two strains (Figure 7).…”

Section: In Vitro Analysis Reveal Strong Antioxidant Capacity Of De111 ®mentioning

confidence: 77%

In vitro and in silico assessment of probiotic and functional properties of Bacillus subtilis DE111®

et al. 2023

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Bacillus subtilis DE111® is a safe, well-tolerated commercially available spore-forming probiotic that has been clinically shown to support a healthy gut microbiome, and to promote digestive and immune health in both adults and children. Recently it was shown that this spore-forming probiotic was capable of germinating in the gastrointestinal tract as early as 3 h after ingestion. However, a better understanding of the mechanisms involved in the efficacy of DE111® is required. Therefore, the present investigation was undertaken to elucidate the functional properties of DE111® through employing a combination of in vitro functional assays and genome analysis. DE111® genome mining revealed the presence of several genes encoding acid and stress tolerance mechanisms in addition to adhesion proteins required to survive and colonize harsh gastrointestinal environment including multi subunit ATPases, arginine deiminase (ADI) pathway genes (argBDR), stress (GroES/GroEL and DnaK/DnaJ) and extracellular polymeric substances (EPS) biosynthesis genes (pgsBCA). DE111® harbors several genes encoding enzymes involved in the metabolism of dietary molecules (protease, lipases, and carbohyrolases), antioxidant activity and genes associated with the synthesis of several B-vitamins (thiamine, riboflavin, pyridoxin, biotin, and folate), vitamin K2 (menaquinone) and seven amino acids including five essential amino acids (threonine, tryptophan, methionine, leucine, and lysine). Furthermore, a combined in silico analysis of bacteriocin producing genes with in vitro analysis highlighted a broad antagonistic activity of DE111® toward numerous urinary tract, intestinal, and skin pathogens. Enzymatic activities included proteases, peptidases, esterase’s, and carbohydrate metabolism coupled with metabolomic analysis of DE111® fermented ultra-high temperature milk, revealed a high release of amino acids and beneficial short chain fatty acids (SCFAs). Together, this study demonstrates the genetic and phenotypic ability of DE111® for surviving harsh gastric transit and conferring health benefits to the host, in particular its efficacy in the metabolism of dietary molecules, and its potential to generate beneficial SCFAs, casein-derived bioactive peptides, as well as its high antioxidant and antimicrobial potential. Thus, supporting the use of DE111® as a nutrient supplement and its pottential use in the preparation of functional foods.

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Section: In Vitro Analysis Reveal Strong Antioxidant Capacity Of De111 ®mentioning

confidence: 77%

In vitro and in silico assessment of probiotic and functional properties of Bacillus subtilis DE111®

et al. 2023

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“…Significant decreases in the proportion of the DNA synthesis phase were observed, accompanied by increases in the G1 phase, representing pre-DNA synthesis. Oxidative stress, caused by a mismatch between free radical production and cellular defense ability, has been confirmed as one of the cytotoxic effects induced by DON in existing studies [ 33 , 34 , 35 ]. It has been reported that RIOK1 was correlated with the transcription factor SKN-1, which is critical for oxidative stress resistance [ 36 , 37 ].…”

Section: Discussionmentioning

confidence: 98%

Analysis of RIOK2 Functions in Mediating the Toxic Effects of Deoxynivalenol in Porcine Intestinal Epithelial Cells

Gao

Fan

et al. 2022

IJMS

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Deoxynivalenol (DON) is a type of mycotoxin that threatens human and livestock health. Right open reading frame kinase 2 (RIOK2) is a kinase that has a pivotal function in ribosome maturation and cell cycle progression. This study aims to clarify the role of the RIOK2 gene in DON-induced cytotoxicity regulation in porcine intestinal epithelial cells (IPEC-J2). Cell viability assay and flow cytometry showed that the knockdown of RIOK2 inhibited proliferation and induced apoptosis, cell cycle arrest, and oxidative stress in DON-induced IPEC-J2. Then, transcriptome profiling identified candidate genes and pathways that closely interacted with both DON cytotoxicity regulation and RIOK2 expression. Furthermore, RIOK2 interference promoted the activation of the MAPK signaling pathway by increasing the phosphorylation of ERK and JNK. Additionally, we performed the dual-luciferase reporter and ChIP assays to elucidate that the expression of RIOK2 was influenced by the binding of transcription factor Sp1 with the promoter region. Briefly, the reduced expression of the RIOK2 gene exacerbates the cytotoxic effects induced by DON in IPEC-J2. Our findings provide insights into the control strategies for DON contamination by identifying functional genes and effective molecular markers.

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“…36 Polyunsaturated fatty acids in biological membranes can be attacked by oxygen radicals, causing lipid peroxidation. 37 As the final product of polyunsaturated fatty acid peroxidation in cells, MDA is widely regarded as a marker of oxidative stress. 37 Previous studies have shown that the level of T-AOC, GSH-Px, and SOD in the immune organs of chickens was decreased and the MDA content was raised by cold stimulation.…”

Section: Discussionmentioning

confidence: 99%

Dietary full-fat rice bran prevents the risk of heart ferroptosis and imbalance of energy metabolism induced by prolonged cold stimulation

Sun

Bao

et al. 2023

Food Funct.

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Lactobacillus rhamnosus GG ameliorates deoxynivalenol-induced kidney oxidative damage and mitochondrial injury in weaned piglets

Abstract: Deoxynivalenol (DON) is a common mycotoxin that pollutes food crops and adversely affects the health of animals, even humans. Lactobacillus rhamnosus GG (LGG) can alleviate intestinal injury, anti-inflammatory and antioxidant...

Cited by 33 publications

References 67 publications

In vitro and in silico assessment of probiotic and functional properties of Bacillus subtilis DE111®

In vitro and in silico assessment of probiotic and functional properties of Bacillus subtilis DE111®

Analysis of RIOK2 Functions in Mediating the Toxic Effects of Deoxynivalenol in Porcine Intestinal Epithelial Cells

Dietary full-fat rice bran prevents the risk of heart ferroptosis and imbalance of energy metabolism induced by prolonged cold stimulation

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