<i>Leishmania tropica</i> successfully treated with photodynamic therapy

Slape, Dana; Kim, Emma; Weller, Paul; Gupta, Monisha

doi:10.1111/ajd.12862

Cited by 10 publications

(4 citation statements)

References 3 publications

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“…The patient was treated with 7 weekly treatments of methyl aminolevulinate occluded for 3 hours and exposed to 8 minutes of red light. 7 Treatment of L tropica infections in children is especially difficult because the organism is resistant to treatment, and systemic drugs are more difficult to administer. In the series by Enk et al, 5 all of the adults with L tropica infections were cured in both the supervised and self-administered groups.…”

Section: Discussionmentioning

confidence: 99%

Leishmania tropica infection of the ear treated with photodynamic therapy

Goldin¹,

Kohen

Taxy

et al. 2020

JAAD Case Reports

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Section: Discussionmentioning

confidence: 99%

Leishmania tropica infection of the ear treated with photodynamic therapy

Goldin¹,

Kohen

Taxy

et al. 2020

JAAD Case Reports

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“…Therefore, PDT may represent an important alternative since it presents minimum side effects and can be easily implemented. Clinical applications of PDT in CL treatment using PS, such as methyl-ALA, is widely reported, indicating the potential of this therapy [8][9][10]. However, it is important to consider that if the treatment cost is low, the access to the therapy for the poorest population will be greater, especially in underdeveloped countries such as Brazil, Colombia, Afghanistan, Peru, and Bolivia [1,11].…”

Section: Introductionmentioning

confidence: 99%

Evaluation of the Photodynamic Therapy with Curcumin on L. braziliensis and L. major Amastigotes

et al. 2021

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Cutaneous leishmaniasis (CL) is a neglected disease prevalent in tropical countries with the ability to cause skin lesions. Photodynamic therapy (PDT) represents a specific and topical option for the treatment of these lesions. This study evaluated the response of macrophages infected with L. braziliensis and L. major to PDT with curcumin. Curcumin concentrations were evaluated in serial dilutions from 500.0 to 7.8 µg/mL using LED (λ = 450 ± 5 nm), with a light dose of 10 J/cm2. The Trypan blue viability test, ultrastructural analysis by scanning electron microscopy (SEM), mitochondrial polarity by Rhodamine 123 (Rho 123), curcumin internalization by confocal microscopy, and counting of the recovered parasites after the PDT treatment were performed. The lowest concentrations of curcumin (15.6 and 7.8 µg/mL) presented photodynamic inactivation. Cell destruction and internalization of curcumin in both macrophages and intracellular parasites were observed in microscopy techniques. In addition, an increase in mitochondrial membrane polarity and a decrease in the number of parasites recovered was observed in the PDT groups. This study indicates that PDT with curcumin has the potential to inactivate infected macrophages and might act as a basis for future in vivo studies using the parameters herein discussed.

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“…tropica . In 2019, Slape et al 14 have stated the successful results of a treatment involving 3 h of methyl aminolevulinate incubation followed by irradiation of red light for 8 min in a Pakistani immigrant with L . tropica after 7 weeks.…”

Section: Introductionmentioning

confidence: 99%

“…In 2016, Fink et al 13 reported the successful results of a 5-week ALA and red light treatment of a Syrian woman with L. tropica. In 2019, Slape et al 14 Methylene blue, a well-known stain (a phenothiazine derivative) with a maximum absorption of light at 665 nm, is effective in PDT, showing an ability to generate 1 O 2 and photodynamic activity for clinical applications against several diseases. 24,25 In the literature, mitochondrial suspensions demonstrated the binding, aggregation, and photochemical properties of MB.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of photodynamic therapy against Leishmania tropica promastigotes using different photosensitizers

Çalışkan

Ertabaklar

Bilgin

et al. 2021

Photoderm Photoimm Photomed

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Background: Photodynamic therapy is a two-step procedure, involving the use of photosensitizing agents followed by selective illumination of the target lesion with visible light. Photodynamic therapy has been described recently as a promising strategy for treatment of leishmaniasis. This study aims to evaluate the in vitro phototoxic, morphological, and apoptotic effect of methylene blue, toluidine blue, chloro-aluminum phthalocyanine, and pheophorbide a-mediated photodynamic therapy on the viability of Leishmania tropica promastigotes.Methods: Parasites were treated with methylene blue, toluidine blue, chloroaluminum phthalocyanine, and pheophorbide a or/and methylene blue, toluidine blue, chloro-aluminum phthalocyanine, and pheophorbide a-mediated photodynamic therapy, and cell proliferation, morphological changes, and apoptosis were evaluated by XTT, giemsa staining, DAPI staining, and DNA fragmentation, respectively.Results: Parasite viability was significantly different in between the groups treated with methylene blue, toluidine blue, and pheophorbide a, with or without irradiation. chloro-aluminum phthalocyanine treatment did not lead to any alterations in cell viability in Leishmania tropica promastigotes with or without irradiation. DAPI staining results indicated that apoptotic bodies and nucleus fragmentation started to be visible in methylene blue, chloro-aluminum phthalocyanine, and pheophorbide a-mediated photodynamic therapy groups. DNA ladder pattern which is used to define apoptosis was observed in irradiated methylene blue, chloro-aluminum phthalocyanine, and pheophorbide a groups. Conclusions:The results revealed that apoptosis-induced cell death was observed in Leishmania tropica promastigotes after the application of photosensitizers in combination with light irradiation.

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Leishmania tropica successfully treated with photodynamic therapy

Cited by 10 publications

References 3 publications

Leishmania tropica infection of the ear treated with photodynamic therapy

Leishmania tropica infection of the ear treated with photodynamic therapy

Evaluation of the Photodynamic Therapy with Curcumin on L. braziliensis and L. major Amastigotes

Evaluation of photodynamic therapy against Leishmania tropica promastigotes using different photosensitizers

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