<i>Leonurus sibiricus</i> root extracts decrease airway remodeling markers expression in fibroblasts

Wieczfińska, Joanna; Sitarek, Przemysław; Kowalczyk, Tomasz; Pawliczak, Rafał

doi:10.1111/cei.13481

Cited by 8 publications

(8 citation statements)

References 112 publications

(142 reference statements)

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“…In this study, infections with two serotypes of rhinoviruses were used—HRV-16 and HRV-2—and resulted in increased mRNA expression of the genes analyzed ( Figure 1 A–H). These results stay in agreement with our previous study [ 26 , 27 , 28 ] and the literature, as it has been reported that infections caused by rhinoviruses are associated with an increase in airway remodeling mediators, e.g., TGF-α, TIMP-2, HGF, MMP-10, LIGHT, IL-1β, GF-1, PDGF, endothelin, and TGF-β [ 3 , 10 , 29 ]. Our aim was to analyze the effect of relaxin on the expression of airway-remodeling genes, depending on whether it was added before or after rhinovirus infection.…”

Section: Discussionsupporting

confidence: 93%

Relaxin Affects Airway Remodeling Genes Expression through Various Signal Pathways Connected with Transcription Factors

Wieczfińska

Pawliczak

2022

IJMS

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Fibrosis is one of the parameters of lung tissue remodeling in asthma. Relaxin has emerged as a natural suppressor of fibrosis, showing efficacy in the prevention of a multiple models of fibrosis. Therefore, the aim of this study was to analyze the aptitudes of relaxin, in the context of its immunomodulatory properties, in the development of airway remodeling. WI-38 and HFL1 fibroblasts, as well as epithelial cells (NHBE), were incubated with relaxin. Additionally, remodeling conditions were induced with two serotypes of rhinovirus (HRV). The expression of the genes contributing to airway remodeling were determined. Moreover, NF-κB, c-Myc, and STAT3 were knocked down to analyze the pathways involved in airway remodeling. Relaxin decreased the mRNA expression of collagen I and TGF-β and increased the expression of MMP-9 (p < 0.05). Relaxin also decreased HRV-induced expression of collagen I and α-SMA (p < 0.05). Moreover, all the analyzed transcription factors—NF-κB, c-Myc, and STAT3—have shown its influence on the pathways connected with relaxin action. Though relaxin requires further study, our results suggest that this natural compound offers great potential for inhibition of the development, or even reversing, of factors related to airway remodeling. The presented contribution of the investigated transcription factors in this process additionally increases its potential possibilities through a variety of its activity pathways.

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Section: Discussionsupporting

confidence: 93%

Relaxin Affects Airway Remodeling Genes Expression through Various Signal Pathways Connected with Transcription Factors

Wieczfińska

Pawliczak

2022

IJMS

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“…Simultaneously, the extracts from Perilla frutescens (L.) Britton (Zisu) (Yang H. et al, 2020), Asparagus cochinchinensis (Lour.) Merr (Tianmendong) (Sung et al, 2017), and Leonurus japonicus Houtt (Yimucao) (Wieczfinska et al, 2020) have also verified their possibilities as a suppressor in airway inflammation and remodeling in asthmatic murine models. The more detailed mechanisms of herbs' efficacy are consulted in Table 2.…”

Section: The Traditional Herbs In Inhibiting Airway Inflammation and ...mentioning

confidence: 88%

“…Fortunately, an increasing number of trials suggested the effective treatment of TCM therapies for airway antiremodeling. Wieczfinska et al (2020) have justified that the Leonurus sibiricus root extracts could significantly decrease airways remodeling marker expression via in vivo studies.…”

Section: Airway Inflammation and Remodeling Factorsmentioning

confidence: 99%

The Role and Mechanisms of Traditional Chinese Medicine for Airway Inflammation and Remodeling in Asthma: Overview and Progress

2022

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Asthma as an individual disease has blighted human health for thousands of years and is still a vital global health challenge at present. Though getting much progress in the utilization of antibiotics, mucolytics, and especially the combination of inhaled corticosteroids (ICS) and long-acting β-agonists (LABA), we are confused about the management of asthmatic airway inflammation and remodeling, which directly threatens the quality of life for chronic patients. The blind addition of ICS will not benefit the remission of cough, wheeze, or sputum, but to increase the risk of side effects. Thus, it is necessary to explore an effective therapy to modulate asthmatic inflammation and airway remodeling. Traditional Chinese Medicine (TCM) has justified its anti-asthma effect in clinical practice but its underlying mechanism and specific role in asthma are still unknown. Some animal studies demonstrated that the classic formula, direct exacts, and natural compounds isolated from TCM could significantly alleviate airway structural alterations and exhibit the anti-inflammatory effects. By investigating these findings and data, we will discuss the possible pathomechanism underlined airway inflammation and remodeling in asthma and the unique role of TCM in the treatment of asthma through regulating different signaling pathways.

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“…According to the World Health Organization (WHO), it is estimated that about 80 percent of the population in developing countries rely on traditionally-used medicinal plants for their primary health care [ 4 ]. Our previous studies showed that extracts from various plant species have biological effects in vitro and in vivo [ 5 , 6 , 7 , 8 ]. Interestingly, the combination of biotechnology in its broadest sense and biological studies have created an efficient platform for the large-scale extraction of valuable secondary metabolites with improved biological properties [ 9 ].…”

Section: Introductionmentioning

confidence: 99%

New Data on Anti-Inflammatory and Wound Healing Potential of Transgenic Senna obtusifolia Hairy Roots: In Vitro Studies

Kowalczyk

Sitarek

Śliwiński

et al. 2023

IJMS

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Asthma is an inflammatory disease whose etiology remains unclear. Its characteristics encompass a wide range of clinical symptoms, inflammatory processes, and reactions to standard therapies. Plants produce a range of constitutive products and secondary metabolites that may have therapeutic abilities. The aim of this study was to determine the effects of Senna obtusifolia transgenic hairy root extracts on virus-induced airway remodeling conditions. Three cell lines were incubated with extracts from transformed (SOA4) and transgenic (SOPSS2, with overexpression of the gene encoding squalene synthase 1) hairy roots of Senna obtusifolia in cell lines undergoing human rhinovirus-16 (HRV-16) infection. The effects of the extracts on the inflammatory process were determined based on the expression of inflammatory cytokines (IL-8, TNF-α, IL-1α and IFN-γ) and total thiol content. The transgenic Senna obtusifolia root extract reduced virus-induced expression of TNF, IL-8 and IL-1 in WI-38 and NHBE cells. The SOPSS2 extract reduced IL-1 expression only in lung epithelial cells. Both tested extracts significantly increased the concentration of thiol groups in epithelial lung cells. In addition, the SOPPS2 hairy root extract yielded a positive result in the scratch test. SOA4 and SOPPS2 Senna obtusifolia hairy root extracts demonstrated anti-inflammatory effects or wound healing activity. The SOPSS2 extract had stronger biological properties, which may result from a higher content of bioactive secondary metabolites.

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Leonurus sibiricus root extracts decrease airway remodeling markers expression in fibroblasts

Cited by 8 publications

References 112 publications

Relaxin Affects Airway Remodeling Genes Expression through Various Signal Pathways Connected with Transcription Factors

Relaxin Affects Airway Remodeling Genes Expression through Various Signal Pathways Connected with Transcription Factors

The Role and Mechanisms of Traditional Chinese Medicine for Airway Inflammation and Remodeling in Asthma: Overview and Progress

New Data on Anti-Inflammatory and Wound Healing Potential of Transgenic Senna obtusifolia Hairy Roots: In Vitro Studies

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