<i>Leptin</i> gene contributes to beef marbling standard, meat brightness, meat firmness, and beef fat standard of the Kumamoto sub‐breed of Japanese Brown cattle

Matsumoto, Hirokazu; Kimura, Satoshi; Nagai, Yohsuke; Fukuda, Yuta; Miyazaki, Kunio; Imai, Satoko; Inenaga, Toshiaki; Kashimura, Atsushi

doi:10.1111/asj.13698

Cited by 4 publications

(4 citation statements)

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“…In 2021, approximately 22,400 animals were reared in Japan. To date, the genetic background [2], growth performance [3], carcass [4,5] and beef characteristics of JBR cattle have been investigated [6,7]. This breed comprises two pedigrees, 10% animals of which are the Kochi (Tosa; JBRT), and the remainder are the Kumamoto [8].…”

Section: Introductionmentioning

confidence: 99%

Metabolomic profiling of postmortem aged muscle in Japanese Brown beef cattle revealed an interbreed difference from Japanese Black beef

et al. 2023

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Objective: Japanese Brown (JBR) cattle, especially the Kochi (Tosa) pedigree (JBRT), is a local breed of moderately marbled beef. Despite the increasing demand, the interbreed differences in muscle metabolites from the highly-marbled Japanese Black (JBL) beef remain poorly understood. We aimed to determine flavorrelated metabolites and postmortem metabolisms characteristic to JBRT beef in comparison with JBL beef.Methods: Lean portions of the longissimus thoracis (LT, loin) muscle from four JBRT cattle were collected at 0, 1, and 14 d postmortem. The muscle metabolomic profiles were analyzed using capillary electrophoresis time-of-flight mass spectrometry. The difference in postmortem metabolisms and aged muscle metabolites were analyzed by statistical and bioinformatic analyses between JBRT (n = 12) and JBL cattle (n = 6).Results: A total of 240 metabolite annotations were obtained from the detected signals of the JBRT muscle samples. Principal component analysis separated the beef samples into three different aging point groups.According to metabolite set enrichment analysis (MSEA), postmortem metabolic changes were associated with the metabolism of pyrimidine, nicotinate and nicotinamide, purine, pyruvate, thiamine, amino sugar, and fatty acid; citric acid cycle; and pentose phosphate pathway as well as various amino acids and mitochondrial fatty acid metabolism. The aged JBRT beef showed higher ultimate pH and lower lactate content than aged JBL beef, suggesting the lower glycolytic activity in postmortem JBRT muscle. JBRT beef was distinguished from JBL beef by significantly different compounds, including choline, amino acids, uridine monophosphate, inosine 5′-monophosphate, fructose 1,6-diphosphate, and betaine, suggesting interbreed differences in the accumulation of nucleotide monophosphate, glutathione metabolism, and phospholipid metabolism. Conclusion:Glycolysis, purine metabolism, fatty acid catabolism, and protein degradation were the most common pathways in beef during postmortem aging. The differentially expressed metabolites and the relevant metabolisms in JBRT beef may contribute to the development of a characteristic flavor.

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Section: Introductionmentioning

confidence: 99%

Metabolomic profiling of postmortem aged muscle in Japanese Brown beef cattle revealed an interbreed difference from Japanese Black beef

et al. 2023

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“…Stepwise selection was conducted to elucidate the factors contributing to all traits as previously reported (Matsumoto et al, 2022). In the model of each trait, SNPs, sex, age (in month: 25.90 ± 1.28 as mean ± SD), slaughtered year (two classes as 2018 and 2019), month (April to July, October, and December), pedigree (first to third sire: 260 classes), and farmer (39 classes) were added as explanatory variables.…”

Section: Discussionmentioning

confidence: 99%

“…Subsequently, association studies between the genotypes and carcass traits (dressed carcass weight, rib‐eye area, rib thickness, subcutaneous fat thickness, beef marbling standard (BMS), beef color standards (BCS), meat brightness, meat firmness, meat texture, beef fat standards (BFS), and fat luster and texture) were conducted using 313 cattle from the sampling population. Detailed information on these 313 cattle has been reported in our previous study (Matsumoto et al, 2022).…”

Section: Methodsmentioning

confidence: 99%

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Causative alleles for chondrodysplastic dwarfism, factor XI deficiency, and factor XIII deficiency in the Kumamoto sub‐breed of Japanese Brown cattle

Matsumoto,

Kimura,

Saito

et al. 2023

Animal Science Journal

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Japanese Brown cattle are the second most popular Wagyu breed, and the Kumamoto sub‐breed shows better daily gain and carcass weight. One of the breeding objectives for this sub‐breed is to reduce genetic defects. Chondrodysplastic dwarfism and factor VIII deficiency have been identified as genetic diseases in the Kumamoto sub‐breed. Previously, we detected individuals in the Kumamoto sub‐breed with causative alleles of genetic diseases identified in Japanese Black cattle. In the current study, 11 mutations responsible for genetic diseases in the Wagyu breeds were analyzed to evaluate the risk of genetic diseases in the Kumamoto sub‐breed. Genotyping revealed the causative mutations of chondrodysplastic dwarfism, factor XI deficiency, and factor XIII deficiency and suggested the appearance of affected animals in this sub‐breed. DNA testing for these diseases is needed to prevent economic loses in beef production using the Kumamoto sub‐breed.

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Polymorphism of the leptin gene (a80v) and its effect on dairy productivity of cattle

Yaryshkin,

Shatalina

2024

BIO Web Conf.

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Modern technologies, including marker breeding, make it possible to identify genetically valuable animals and select individuals for breeding and raising, which in turn helps to increase productivity, improve the quality of milk and dairy products and reduce material costs for its production. The researchers also note the relationship of the leptin gene with an increase in live weight of cattle and lipid metabolism. The purpose of the research was to study the DNA polymorphism of the leptin marker gene and its possible use as a leptin marker gene to improve the productive performance of cattle. The animals were genotyped using PCR-RFLP analysis. Data on milk productivity and insemination indicators are taken from the ARM "Selex" (dairy cattle) program. Biometric processing of the obtained results was carried out, the reliability criterion was calculated. The results of the study showed that the AA genotype and the A allele are the most common in the Holstein breed. It was concluded that the AA genotype is associated with high milk yield, and the AV genotype is associated with an increase in fat and protein in milk and an increase in body weight. The milk yield of these individuals exceeds the indicators of their peers by 200-400 kg, and in terms of lifetime productivity reaches 4,600 kg. The AA genotype has a beneficial effect on the timing of economically beneficial use of cows. The difference in the duration of economic use was 0.6-2.1 lactation. The use of marker genes, such as leptin A80V, can help increase productivity and improve milk quality in cattle, which is an important task of the breeding process.

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Leptin gene contributes to beef marbling standard, meat brightness, meat firmness, and beef fat standard of the Kumamoto sub‐breed of Japanese Brown cattle

Cited by 4 publications

References 32 publications

Metabolomic profiling of postmortem aged muscle in Japanese Brown beef cattle revealed an interbreed difference from Japanese Black beef

Metabolomic profiling of postmortem aged muscle in Japanese Brown beef cattle revealed an interbreed difference from Japanese Black beef

Causative alleles for chondrodysplastic dwarfism, factor XI deficiency, and factor XIII deficiency in the Kumamoto sub‐breed of Japanese Brown cattle

Polymorphism of the leptin gene (a80v) and its effect on dairy productivity of cattle

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