<i>Linc1557</i> is critical for the initiation of embryonic stem cell differentiation by directly targeting the LIF/STAT3 signaling pathway

Lan, Yuanyuan; Lu, Chenqi; Yang, Yiwei; Liu, Xiaoqin; Guo, Xudong; Xi, Jiajie; Kang, Jiuhong

doi:10.1002/stem.3130

Cited by 8 publications

(6 citation statements)

References 41 publications

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“…55 Additionally, increasing evidence has indicated that lncRNA or miRNA activate the LIF/STAT3 pathway to regulate cellular state and function. 57,[60][61][62] The most significant result of this study indicated that lncRNA-412.25 activated the LIF/STAT3 pathway-mediated GCs state by interacting with miR-346 in Hu sheep.…”

Section: Discussionmentioning

confidence: 99%

LncRNA‐412.25 activates the LIF/STAT3 signaling pathway in ovarian granulosa cells of Hu sheep by sponging miR‐346

Yao

El-Samahy

et al. 2022

The FASEB Journal

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Litter size, an important economic trait, determines the fecundity and economic value of sheep bred for meat. Moreover, ovulation rate is directly correlated with the sheep litter size 1 and that is regulated by folliculogenesis and oocyte maturation, which are extremely complex processes dependent on the integration of the endocrine system and intraovarian factors. Therefore, elucidation of the molecular mechanism underlying ovulation and follicular

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Section: Discussionmentioning

confidence: 99%

LncRNA‐412.25 activates the LIF/STAT3 signaling pathway in ovarian granulosa cells of Hu sheep by sponging miR‐346

Yao

El-Samahy

et al. 2022

The FASEB Journal

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“…For instance, lncRNA H19 suppresses the differentiation of Th17 cells and proliferation of ESCs through miR-342-3p/IER3 pathway [ 19 ]. Linc1557 inhibits the differentiation of ESCs by regulating the phosphorylation levels of STAT3 and affecting its stability [ 20 ]. However, the roles and mechanisms of lncRNAs regulate the differentiation of ESCs into pacemaker-like cells is still unclear.…”

Section: Discussionmentioning

confidence: 99%

Downregulated lncRNA RCPCD promotes differentiation of embryonic stem cells into cardiac pacemaker-like cells by suppressing HCN4 promoter methylation

Zhu

You²,

Wei

et al. 2021

Cell Death Dis

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Long non-coding RNA (lncRNA) is receiving increasing attention in embryonic stem cells (ESCs) research. However, the roles of lncRNA in the differentiation of ESCs into pacemaker-like cells are still unclear. Therefore, the present study aims to explore the roles and mechanisms of lncRNA in the differentiation of ESCs into pacemaker-like cells. ESCs were cultured and induced differentiation to pacemaker-like cells. RNA sequencing was used to identify the differential expression lncRNAs during the differentiation of ESCs into pacemaker-like cells. Cell morphology observation, flow cytometry, quantitative real-time polymerase chain reaction, western blot, and immunofluorescence were used to detect the differentiation of ESCs into pacemaker-like cells. LncRNA and genes overexpression or knockdown through transfected adenovirus in the differentiation process. The fluorescence in situ hybridization (FISH) detected the lncRNA location in the differentiated ESCs. Luciferase reporter gene assay, methylation-specific PCR, chromatin immunoprecipitation assay, and RNA immunoprecipitation assay were performed to reveal the mechanism of lncRNA-regulating HCN4 expression. Rescue experiments were used to confirm that lncRNA regulates the differentiation of ESCs into pacemaker-like cells through HCN4. We cultured the ESCs and induced the differentiation of ESCs into pacemaker-like cells successfully. The expression of lncRNA RCPCD was significantly decreased in the differentiation of ESCs into pacemaker-like cells. Overexpression of RCPCD inhibited the differentiation of ESCs into pacemaker-like cells. RCPCD inhibited the expression of HCN4 by increasing HCN4 methylation at the promoter region through DNMT1, DNMT2, and DNMT3. RCPCD inhibited the differentiation of ESCs into pacemaker-like cells by inhibiting the expression of HCN4. Our results confirm the roles and mechanism of lncRNA RCPCD in the differentiation of ESCs into pacemaker-like cells, which could pave the path for the development of a cell-based biological pacemaker.

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“…According to reports, the leukemia inhibitory factor (LIF)/STAT3 signaling pathway is essential for maintaining the self-renewal and pluripotency of mESCs [ 59 ]. Linc1557 is mainly located in the cytoplasm of mESCs and directly interacts with STAT3 through specific binding sites to regulate the stability of its mRNA, regulating the LIF/STAT3 signaling pathway and promoting the self-renewal of mESCs [ 60 ]. Peptidylprolyl Isomerase E (PPIE) is an RNA-binding protein located in the nucleus of hESCs [ 61 ].…”

Section: The Functions Of Lncrnas and Their Rbps In Stem Cellsmentioning

confidence: 99%

LncRNAs and their RBPs: How to influence the fate of stem cells?

Zhao

Xie

Zhu³

et al. 2022

Stem Cell Res Ther

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Stem cells are distinctive cells that have self-renewal potential and unique ability to differentiate into multiple functional cells. Stem cell is a frontier field of life science research and has always been a hot spot in biomedical research. Recent studies have shown that long non-coding RNAs (lncRNAs) have irreplaceable roles in stem cell self-renewal and differentiation. LncRNAs play crucial roles in stem cells through a variety of regulatory mechanisms, including the recruitment of RNA-binding proteins (RBPs) to affect the stability of their mRNAs or the expression of downstream genes. RBPs interact with different RNAs to regulate gene expression at transcriptional and post-transcriptional levels and play important roles in determining the fate of stem cells. In this review, the functions of lncRNAs and their RBPs in self-renewal and differentiation of stem cell are summarized. We focus on the four regulatory mechanisms by which lncRNAs and their RBPs are involved in epigenetic regulation, signaling pathway regulation, splicing, mRNA stability and subcellular localization and further discuss other noncoding RNAs (ncRNAs) and their RBPs in the fate of stem cells. This work provides a more comprehensive understanding of the roles of lncRNAs in determining the fate of stem cells, and a further understanding of their regulatory mechanisms will provide a theoretical basis for the development of clinical regenerative medicine.

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Linc1557 is critical for the initiation of embryonic stem cell differentiation by directly targeting the LIF/STAT3 signaling pathway

Cited by 8 publications

References 41 publications

LncRNA‐412.25 activates the LIF/STAT3 signaling pathway in ovarian granulosa cells of Hu sheep by sponging miR‐346

LncRNA‐412.25 activates the LIF/STAT3 signaling pathway in ovarian granulosa cells of Hu sheep by sponging miR‐346

Downregulated lncRNA RCPCD promotes differentiation of embryonic stem cells into cardiac pacemaker-like cells by suppressing HCN4 promoter methylation

LncRNAs and their RBPs: How to influence the fate of stem cells?

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