<i>Lippia origanoides</i>
            essential oil increases longevity and ameliorates β-amyloid peptide-induced toxicity in
            <i>Caenorhabditis elegans</i>

Henrique Moniz, Ana Maria; Xavier Junior, Francisco Humberto; Melo Martins Silva, Giovanna; Reis de Melo, Ana Cristina Gonçalves; Silva, Maylla Maria Correia Leite; Paiva, Weslley Souza; Rocha, Hugo Alexandre Oliveira; da Costa, Luiz Antônio Mendonça Alves; Melo Filho, Antônio Alves de; Oliveira, Riva de Paula

doi:10.1080/14786419.2023.2287183

Cited by 2 publications

(2 citation statements)

References 49 publications

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“…In recent years, researchers have made good progress using these transgenic C. elegans models to conduct a number of AD-related studies. For example, Lippia origanoides essential oil (LOEO) ( Henrique Moniz et al, 2023 ), Hesperidin ( Kumar et al, 2023 ), A fruit extract of Styphnolobium japonicum (L.) ( Thabit et al, 2023 ) have also been found to be efficacious in the treatment of AD in nematode model-based studies. Overall, the practice of AD drug development using the C. elegans model has become more mature, providing a bright prospect for high-throughput screening of drugs for neurodegenerative diseases ( Paul et al, 2020 ).…”

Section: Discussionmentioning

confidence: 99%

P-hydroxybenzaldehyde protects Caenorhabditis elegans from oxidative stress and β-amyloid toxicity

Yu,

Tao,

Xiao

et al. 2024

Front. Aging Neurosci.

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IntroductionGastrodia elata is the dried tuber of the orchid Gastrodia elata Bl. It is considered a food consisting of a source of precious medicinal herbs, whose chemical composition is relatively rich. Gastrodia elata and its extracted fractions have been shown to have neuroprotective effects. P-hydroxybenzaldehyde (p-HBA), as one of the main active components of Gastrodia elata, has anti-inflammatory, antioxidative stress, and cerebral protective effects, which has potential for the treatment of Alzheimer’s disease (AD). The aim of this study was to verify the role of p-HBA in AD treatment and to investigate its mechanism of action in depth based using the Caenorhabditis elegans (C. elegans) model.MethodsIn this study, we used paralysis, lifespan, behavioral and antistress experiments to investigate the effects of p-HBA on AD and aging. Furthermore, we performed reactive oxygen species (ROS) assay, thioflavin S staining, RNA-seq analysis, qPCR validation, PCR Array, and GFP reporter gene worm experiment to determine the anti-AD effects of p-HBA, as well as in-depth studies on its mechanisms.Resultsp-HBA was able to delay paralysis, improve mobility and resistance to stress, and delay aging in the AD nematode model. Further mechanistic studies showed that ROS and lipofuscin levels, Aβ aggregation, and toxicity were reduced after p-HBA treatment, suggesting that p-HBA ameliorated Aβ-induced toxicity by enhancing antioxidant and anti-aging activity and inhibiting Aβ aggregation. p-HBA had a therapeutic effect on AD by improving stress resistance, as indicated by the down-regulation of NLP-29 and UCR-11 expression and up-regulation of PQN-75 and LYS-3 expression. In addition, the gene microarray showed that p-HBA treatment played a positive role in genes related to AD, anti-aging, ribosomal protein pathway, and glucose metabolism, which were collectively involved in the anti-AD mechanism of p-HBA. Finally, we also found that p-HBA promoted nuclear localization of DAF-16 and increased the expression of SKN-1, SOD-3, and GST-4, which contributed significantly to inhibition of Aβ toxicity and enhancement of antioxidative stress.ConclusionOur work suggests that p-HBA has some antioxidant and anti-aging activities. It may be a viable candidate for the treatment and prevention of Alzheimer’s disease.

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Section: Discussionmentioning

confidence: 99%

P-hydroxybenzaldehyde protects Caenorhabditis elegans from oxidative stress and β-amyloid toxicity

Yu,

Tao,

Xiao

et al. 2024

Front. Aging Neurosci.

View full text Add to dashboard Cite

show abstract

“…The unique advantages of C. elegans have allowed its wide use in screening for natural compounds with potential anti-AD effects. For instance, studies based on this model have demonstrated the antioxidant and anti-aging properties of Lippia origanoides essential oil, and highlighted its potential for the prevention and treatment of AD ( Henrique Moniz et al, 2023 ).…”

Section: Discussionmentioning

confidence: 99%

4,4′-methylenediphenol reduces Aβ-induced toxicity in a Caenorhabditis elegans model of Alzheimer’s disease

Yu,

Tao,

Xiao

et al. 2024

Front. Aging Neurosci.

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IntroductionGastrodia elata Blume is a widely used medicinal and edible herb with a rich chemical composition. Moreover, prescriptions containing Gastrodia elata are commonly used for the prevention and treatment of cardiovascular, cerebrovascular, and aging-related diseases. Recent pharmacological studies have confirmed the antioxidant and neuroprotective effects of Gastrodia elata, and, in recent years, this herb has also been used in the treatment of Alzheimer’s disease (AD) and other neurodegenerative disorders. We have previously shown that 4,4′-methylenediphenol, a key active ingredient of Gastrodia elata, can mitigate amyloid-β (Aβ)-induced paralysis in AD model worms as well as prolong the lifespan of the animals, thus displaying potential as a treatment of AD.MethodsWe investigated the effects of 4,4′-methylenediphenol on AD and aging through paralysis, lifespan, and behavioral assays. In addition, we determined the anti-AD effects of 4,4′-methylenediphenol by reactive oxygen species (ROS) assay, lipofuscin analysis, thioflavin S staining, metabolomics analysis, GFP reporter gene worm assay, and RNA interference assay and conducted in-depth studies on its mechanism of action.Results4,4′-Methylenediphenol not only delayed paralysis onset and senescence in the AD model worms but also enhanced their motility and stress tolerance. Meanwhile, 4,4′-methylenediphenol treatment also reduced the contents of reactive oxygen species (ROS) and lipofuscin, and decreased Aβ protein deposition in the worms. Broad-spectrum targeted metabolomic analysis showed that 4,4′-methylenediphenol administration had a positive effect on the metabolite profile of the worms. In addition, 4,4′-methylenediphenol promoted the nuclear translocation of DAF-16 and upregulated the expression of SKN-1, SOD-3, and GST-4 in the respective GFP reporter lines, accompanied by an enhancement of antioxidant activity and a reduction in Aβ toxicity; importantly, our results suggested that these effects of 4,4′-methylenediphenol were mediated, at least partly, via the activation of DAF-16.ConclusionWe have demonstrated that 4,4′-methylenediphenol can reduce Aβ-induced toxicity in AD model worms, suggesting that it has potential for development as an anti-AD drug. Our findings provide ideas and references for further research into the anti-AD effects of Gastrodia elata and its active ingredients.

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Lippia origanoides essential oil increases longevity and ameliorates β-amyloid peptide-induced toxicity in Caenorhabditis elegans

Cited by 2 publications

References 49 publications

P-hydroxybenzaldehyde protects Caenorhabditis elegans from oxidative stress and β-amyloid toxicity

P-hydroxybenzaldehyde protects Caenorhabditis elegans from oxidative stress and β-amyloid toxicity

4,4′-methylenediphenol reduces Aβ-induced toxicity in a Caenorhabditis elegans model of Alzheimer’s disease

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