Listeriacontamination in chevon and mutton from retail meat shops and slaughter house environment of Punjab, India

Abstract

Listeria monocytogenes is recognized as an emerging pathogen, with limited information or statistics on this pathogen. Moreover, poor hygienic practices in the unorganized meat sector favor its growth and survival. Therefore, the present study was carried out in Punjab, India to assess the prevalence of Listeria spp. in chevon and mutton samples, followed by its characterization. The prevalence of Listeria in chevon, mutton and swab samples collected from butchers’ shops and slaughter houses was 1.82%, 3.21% and 6.66%, respectively. The 18 (2.67%) L. monocytogenes strains isolated from 786 samples carried one or more virulence-associated gene. Based on virulence genes, 6/18 isolates were potentially pathogenic. Serovar 4b (44.4%) was predominant, followed by serovars 1/2a (22.2%), 1/2b (22.2%) and 1/2c (11.1%). Detection of L. monocytogenes in meat and environment samples indicated poor hygiene, potential cross-contamination and risk of listeriosis to consumers and occupational workers. The findings of the study were also relevant to the fact that most of the slaughtering in Punjab, India is carried out in unorganized way, and this meat is not stored propery. Moreover, in the absence of a systematic foodborne disease surveillance and monitoring program, a database on foodborne diseases is absent, and needs to be established.

Ecology of Listeria monocytogenes and Listeria species in India: the occurrence, resistance to biocides, genomic landscape and biocontrol

“…Also, L. innocua (0.90%), L. ivanovii (0.40%), and L. monocytogenes (0.10%) were isolated from beef carcasses, knives, cutting table surfaces, and water samples collected from cattle slaughterhouses of Kerala (Gowda et al ., 2017). Pathogenic L. monocytogenes belonging to 4b, 1/2a, 1/2b, and 1/2c serotypes were isolated from the chevon, mutton, and swab samples collected from the slaughterhouses of Punjab (Alka et al ., 2019). Listeria monocytogenes was isolated from chicken (8.0%), fish (10.0%) and chevon (4.0%) (Sharma et al ., 2021).…”