<i>Listeria monocytogenes</i>: Antibiotic Resistance in Food Production

Lungu, Bwalya; O’Bryan, Corliss A.; Muthaiyan, Arunachalam; Milillo, Sara R.; Johnson, Michael G.; Crandall, Philip G.; Ricke, Steven C.

doi:10.1089/fpd.2010.0718

Cited by 86 publications

(60 citation statements)

References 104 publications

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“…This resistance profile is relatively common in Europe and North America [11,24,25] and is consistent with the identification of the tet, aad, and erm resistance genes in Listeria spp. [13]. In Brazil, this resistance profile has not been commonly observed, and Listeria isolates are susceptible to most antibiotics [26][27][28].…”

Section: Discussionmentioning

confidence: 99%

“…However, increasing reports of Listeria strains isolated from various sources that are resistant to penicillin, ampicillin, tetracycline, streptomycin, clindamycin, and even oxacillin and vancomycin represent a major public health concern [9][10][11]. The genetic mechanisms responsible for these resistances are an important research topic, given that L. monocytogenes can acquire resistance genes from, and transfer them to, enterococci, staphylococci, and streptococci [12,13].…”

Section: Introductionmentioning

confidence: 99%

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Characterization of antibiotic resistance in Listeria spp. isolated from slaughterhouse environments, pork and human infections

Moreno

Paixão

Gobbi

et al. 2014

J Infect Dev Ctries

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Introduction: Listeria species are susceptible to most antibiotics. However, over the last decade, increasing reports of multidrug-resistant Listeria spp. from various sources have prompted public health concerns. The objective of this study was to characterize the antibiotic susceptibility of Listeria spp. and the genetic mechanisms that confer resistance. Methodology: Forty-six Listeria spp. isolates were studied, and their minimal inhibitory concentrations of antibiotics were determined by microdilution using Sensititre standard susceptibility MIC plates. The isolates were screened for the presence of gyrA, parC, lde, lsa(A), lnu(A), and mprF by PCR, and the amplified genes were sequenced. Results: All isolates were susceptible to penicillin, ampicillin, tetracycline, erythromycin, and carbapenems. Resistance to clindamycin, daptomycin, and oxacillin was found among L. monocytogenes and L. innocua, and all species possessed at least intermediate resistance to fluoroquinolones. GyrA, parC, and mprF were detected in all isolates; however, mutations were found only in gyrA sequences. A high daptomycin MIC, as reported previously, was observed, suggesting an intrinsic resistance of Listeria spp. to daptomycin. Conclusions: These results are consistent with reports of emerging resistance in Listeria spp. and emphasize the need for further genotypic characterization of antibiotic resistance in this genus.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Characterization of antibiotic resistance in Listeria spp. isolated from slaughterhouse environments, pork and human infections

Moreno

Paixão

Gobbi

et al. 2014

J Infect Dev Ctries

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“…The low incidence of antibiotic resistance in L. monocytogenes is a distinctive feature of the microorganism, which is also observed in the food production chain (Lungu et al, 2011). Nevertheless, there still exists the risk of foodborne infections due to resistant L. monocytogenes strains isolated from livestock products and their related environments.…”

Section: Infected Animalsmentioning

confidence: 99%

Review. Dairy farm management and production practices associated with the presence of Listeria monocytogenes in raw milk and beef

Santorum

Garcia

López-Alonso

et al. 2012

Span. j. agric. res.

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Human listeriosis is a severe foodborne disease caused by Listeria monocytogenes. It is a zoonosis that represents a significant concern for the food industry due to the high mortality rate it causes and the fact that the organism is capable of growing at refrigeration temperatures. Dairy products and ready-to-eat meats are among the foods most often involved in listeriosis outbreaks. Listeria is a common contaminant in the dairy environment, both on the farm and in the processing plant. The main sources of L. monocytogenes in dairy farms are manure and improperly fermented silage. If silage crops are grown on contaminated land, a new cycle of silage contamination and faecal shedding by ruminants that consume such silage may ensue. High loads of L. monocytogenes produced in farm environments may thus represent a primary source for the introduction of this pathogen into the human food supply chain; dairy cows would represent a reservoir for the bacterium, and raw milk and beef would represent the main vehicles for its transmission from dairy farms to humans. Even if contamination originates in post-processing environments, contaminated raw foods may still represent a vehicle for introducing L. monocytogenes into food processing plants. Molecular typing methods have confirmed that common strains of L. monocytogenes are present in dairy farm-associated isolates and isolates from both human epidemic and sporadic cases. Pre-harvest (on-farm) control of listeriosis should be based mainly on the control of manure, silage, herd health and milking practices.Additional key words: animal reservoir; dairy primary production; environmental sources; food vehicles; listeriosis. ResumenRevisión. Tipos de gestión y producción de las granjas de ganado vacuno lechero relacionados con la presencia de Listeria monocytogenes en la leche y la carne La listeriosis humana es una grave enfermedad transmitida por alimentos y causada por Listeria monocytogenes. Se trata de una zoonosis que supone una gran preocupación para la industria alimentaria debido a su alta tasa de mortalidad y al hecho de que el microorganismo es capaz de crecer a temperaturas de refrigeración. Listeria es un contaminante habitual en las granjas y plantas de productos lácteos. En las granjas las fuentes principales de L. monocytogenes son el estiércol y los ensilados mal fermentados. Si la cosecha utilizada para producir ensilados procede de campos contaminados, puede comenzar un nuevo ciclo de contaminación de los ensilados y liberación del patógeno en las heces de los animales. El ganado vacuno lechero representa uno de los principales reservorios de este microorganismo y la leche y la carne representarían los principales vehículos para su transmisión desde la granja de producción de leche al ser humano. Incluso cuando la contaminación del alimento procede de etapas posteriores al procesado, los alimentos crudos contaminados también representan uno de los vehículos de entrada de L. monocytogenes en las plan-

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“…13,14 In addition, Proteus vulgaris has the capacity to resist antibiotic treatment due to the inducible expression of chromosomal β-lactamases, 15 while Listeria monocytogenes showed resistance to antibiotic treatment that could be acquired through self-transferable plasmids, mobilizable plasmids, and conjugative transposons. 16 Biofilm formation is an important factor in bacterial virulence. It follows the adherence of the bacteria to abiotic or biotic surfaces, and increases the ability of bacteria to survive environmental stress and enhances protection against bactericidal effects.…”

mentioning

confidence: 99%

Efficacy of neutral and negatively charged liposome-loaded gentamicin on planktonic bacteria and biofilm communities

Alhariri¹,

Majrashi²,

Bahkali³

et al. 2017

IJN

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We investigated the efficacy of liposomal gentamicin formulations of different surface charges against Pseudomonas aeruginosa and Klebsiella oxytoca . The liposomal gentamicin formulations were prepared by the dehydration–rehydration method, and their sizes and zeta potential were measured. Gentamicin encapsulation efficiency inside the liposomal formulations was determined by microbiologic assay, and stability of the formulations in biologic fluid was evaluated for a period of 48 h. The minimum inhibitory concentration and the minimum bactericidal concentration were determined, and the in vitro time kill studies of the free form of gentamicin and liposomal gentamicin formulations were performed. The activities of liposomal gentamicin in preventing and reducing biofilm-forming P. aeruginosa and K. oxytoca were compared to those of free antibiotic. The sizes of the liposomal formulations ranged from 625 to 806.6 nm in diameter, with the zeta potential ranging from −0.22 to −31.7 mV. Gentamicin encapsulation efficiency inside the liposomal formulation ranged from 1.8% to 43.6%. The liposomes retained >60% of their gentamicin content during the 48 h time period. The minimum inhibitory concentration of neutral formulation was lower than that of free gentamicin (0.25 versus 1 mg/L for P. aeruginosa and 0.5 versus 1 mg/L for K. oxytoca ). The negatively charged formulation exhibited the same bacteriostatic concentration as that of free gentamicin. The minimum bactericidal concentration of neutral liposomes on planktonic bacterial culture was twofold lower than that of free gentamicin, whereas the negatively charged formulations were comparable to free gentamicin. The killing time curve values for the neutral negatively charged formulation against planktonic P. aeruginosa and K. oxytoca were better than those of free gentamicin. Furthermore, liposomal formulations prevent the biofilm-formation ability of these strains better than free gentamicin. In summary, liposomal formulations could be an effective lipid nanoparticle to combat acute infections where planktonic bacteria are predominant.

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Listeria monocytogenes: Antibiotic Resistance in Food Production

Cited by 86 publications

References 104 publications

Characterization of antibiotic resistance in Listeria spp. isolated from slaughterhouse environments, pork and human infections

Characterization of antibiotic resistance in Listeria spp. isolated from slaughterhouse environments, pork and human infections

Review. Dairy farm management and production practices associated with the presence of Listeria monocytogenes in raw milk and beef

Efficacy of neutral and negatively charged liposome-loaded gentamicin on planktonic bacteria and biofilm communities

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