<i>Listeria monocytogenes</i>: Cultivation and Laboratory Maintenance

Jones, Grant S.; D’Orazio, Sarah E. F.

doi:10.1002/9780471729259.mc09b02s31

Cited by 38 publications

(33 citation statements)

References 28 publications

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“…Organism can be identified on the basis of Gram-staining of smear/culture, motility, haemolysis, biochemical tests (catalase reaction), peroxide-anti-peroxide method, sugar utilization, immunofluorescence test (IFT), or DNA analysis (AOAC 2000;Jones & D'Orazio 2013;OIE 2014). Anton test is also used for detecting Listeria where the infected material is dropped on the conjunctiva of an eye of a rabbit that produces conjunctivitis or kerato-conjunctivitis within 24À36 hours.…”

Section: Diagnosismentioning

confidence: 99%

Listeriosis in animals, its public health significance (food-borne zoonosis) and advances in diagnosis and control: a comprehensive review

Dhama

Karthik

Tiwari

et al. 2015

Veterinary Quarterly

133

108

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Listeriosis is an infectious and fatal disease of animals, birds, fish, crustaceans and humans. It is an important food-borne zoonosis caused by Listeria monocytogenes, an intracellular pathogen with unique potential to spread from cell to cell, thereby crossing blood-brain, intestinal and placental barriers. The organism possesses a pile of virulence factors that help to infect the host and evade from host immune machinery. Though disease occurrence is sporadic throughout the world, it can result in severe damage during an outbreak. Listeriosis is characterized by septicaemia, encephalitis, meningitis, meningoencephalitis, abortion, stillbirth, perinatal infections and gastroenteritis with the incubation period varying with the form of infection. L. monocytogenes has been isolated worldwide from humans, animals, poultry, environmental sources like soil, river, decaying plants, and food sources like milk, meat and their products, seafood and vegetables. Since appropriate vaccines are not available and infection is mainly transmitted through foods in humans and animals, hygienic practices can prevent its spread. The present review describes etiology, epidemiology, transmission, clinical signs, post-mortem lesions, pathogenesis, public health significance, and advances in diagnosis, vaccines and treatment of this disease. Special attention has been given to novel as well as prospective emerging therapies that include bacteriophage and cytokine therapy, avian egg yolk antibodies and herbal therapy. Various vaccines, including advances in recombinant and DNA vaccines and their modes of eliciting immune response, are also discussed. Due focus has also been given regarding appropriate prevention and control strategies to be adapted for better management of this zoonotic disease.

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Section: Diagnosismentioning

confidence: 99%

Listeriosis in animals, its public health significance (food-borne zoonosis) and advances in diagnosis and control: a comprehensive review

Dhama

Karthik

Tiwari

et al. 2015

Veterinary Quarterly

133

108

View full text Add to dashboard Cite

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“…L. monocytogenes need to be grown in a rich culture medium and BHI is the most commonly used non-selective media for cultivation of Listeria species (Jones and D'Orazio 2005). Later, L. monocytogenes was spread on Oxford agar (Listeria selective agar, Oxoid, with Listeria selective supplement) at 37 C for 48h to check for purity.…”

Section: Challenge Test and Microbiological Analysismentioning

confidence: 99%

Inhibition ofListeria monocytogenesby a formulation of selected dairy starter cultures and probiotics in anin vitromodel

Iulietto

Sechi

Cella

et al. 2018

Italian Journal of Animal Science

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Three strains of lactic acid bacteria (LAB) and a commercial probiotic were selected to evaluate their in vitro activity towards Listeria monocytogenes. The strains Lactococcus lactis ssp. lactis, strain 340, L. lactis ssp. lactis, strain 16; Lactobacillus casei ssp. casei, strain 208 and Enterococcus faecium UBEF-41 were inoculated into skim milk and brain heart infusion broth (BHI) to get an initial Lactococcus: Lactobacillus: E. faecium UBEF-41 ratio of 2:1:1 and a concentration of approximately 7 log cfu mL À1 until challenge vs. pathogen. L. monocytogenes ATCC 7644 was also inoculated in same media to get approximately 4 log cfu mL À1 . Growth curves in skim milk and BHI at 4, 10 and 30 C, respectively were studied for: (i) LAB formulation; (ii) L. monocytogenes and (iii) LAB vs. L. monocytogenes. When challenged with LAB, at 30 C in milk, L. monocytogenes was not detectable after day-3 and in BHI it decreased below log cfu mL À1 after day-5. At 10 and 4 C, in both media, L. monocytogenes counts were always significantly lower (p < .001) than the counts of L. monocytogenes alone from day-2 for milk at 4 C and BHI at 10 C and from day-7 for BHI at 4C and milk at 10 C. In conclusion, the proposed formulation was able to limit L. monocytogenes in vitro growth, even at refrigeration temperature. ARTICLE HISTORY

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“…Intestinally passaged Lm were grown to early stationary phase in Brain Heart Infusion (BHI) broth (Difco) shaking at 37° C (for i.v. infection) or standing at 30° C (for foodborne infection) and then aliquots were prepared and frozen at −80° C until use as described previously (23). …”

Section: Methodsmentioning

confidence: 99%

Type I IFN Does Not Promote Susceptibility to Foodborne Listeria monocytogenes

Pitts

Myers-Morales

D’Orazio

2016

The Journal of Immunology

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Type I IFN (IFNα/β) is thought to enhance growth of the foodborne intracellular pathogen Listeria monocytogenes (Lm) by promoting mechanisms that dampen innate immunity to infection. However, the type I IFN response has been studied primarily using methods that bypass the stomach and, therefore, fail to replicate the natural course of Lm infection. In this study, we compared i.v. and foodborne transmission of Lm in mice lacking the common type I IFN receptor (IFNAR1−/−). Contrary to what was observed using i.v. infection, IFNAR1−/− and wild type mice had similar bacterial burdens in the liver and spleen following foodborne infection. Splenocytes from wild type mice infected intravenously produced significantly more IFNβ than those infected by the foodborne route. Consequently, the immunosuppressive effects of type I IFN signaling, which included T cell death, increased IL-10 secretion, and repression of neutrophil recruitment to the spleen, were all observed following i.v., but not foodborne transmission of Lm. Type I IFN was also previously shown to cause a loss of responsiveness to IFNγ through down-regulation of the receptor IFNGR1 on macrophages and dendritic cells. However, we detected a decrease in surface expression of IFNGR1 even in the absence of IFNα/β signaling, suggesting that in vivo, this infection-induced phenotype is not type I IFN-dependent. These results highlight the importance of using the natural route of infection for studies of host-pathogen interactions and suggest that the detrimental effects of IFNα/β signaling on the innate immune response to Lm may be an artifact of the i.v. infection model.

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Listeria monocytogenes: Cultivation and Laboratory Maintenance

Cited by 38 publications

References 28 publications

Listeriosis in animals, its public health significance (food-borne zoonosis) and advances in diagnosis and control: a comprehensive review

Listeriosis in animals, its public health significance (food-borne zoonosis) and advances in diagnosis and control: a comprehensive review

Inhibition ofListeria monocytogenesby a formulation of selected dairy starter cultures and probiotics in anin vitromodel

Type I IFN Does Not Promote Susceptibility to Foodborne Listeria monocytogenes

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