Listeria monocytogenesisolates from food and food environment harbouringtetMandermBresistance genes

Haubert, Louise; Mendonça, Mercelo L; Lopes, Graciela Völz; Cardoso, Marisa Ribeiro de Itapema; Silva, W.P. da

doi:10.1111/lam.12516

Cited by 52 publications

(29 citation statements)

References 39 publications

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“…With respect to the antimicrobial susceptibility assays, results revealed low levels of antibiotic resistance. The exceptions were: i) cefoxitin and cefepime-not surprising as LM is naturally resistant to subclasses of cephems, especially cephalosporin [45]; ii) sulfonamides-this phenotype is of importance as the combination of trimethoprim and sulfonamides is the treatment of choice against listeriosis in penicillin-allergic patients [46]. However, resistance to the combination of trimethoprim and sulfamethoxazole was null in the present study.…”

Section: Discussioncontrasting

confidence: 56%

Listeria monocytogenes in Export-approved Beef from Mato Grosso, Brazil: Prevalence, Molecular Characterization and Resistance to Antibiotics and Disinfectants

et al. 2019

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The Brazilian state of Mato Grosso is the largest producer and exporter of beef in the country, but few studies of relevance have been conducted to evaluate the microbiological safety of its products. This study aimed to estimate the prevalence of Listeria monocytogenes (LM) in export-approved beef from Mato Grosso and to characterize the isolates in terms of molecular properties and antimicrobial resistance. From a total of 50 samples analyzed, Listeria sp. was isolated in 18 (36% prevalence). Listeria monocytogenes was confirmed in 6 (12% prevalence). Among the serotype groups assessed by multiplex PCR, serotype 4 (4b, 4d or 4e) was the most prevalent. Although antibiotic resistance was not an issue, two strains isolated from different plants showed high resistance to sodium hypochlorite. Overall, this scenario causes concern because it puts at risk not only the Brazilian customer, but also the population of countries that import beef from Mato Grosso.

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Section: Discussioncontrasting

confidence: 56%

Listeria monocytogenes in Export-approved Beef from Mato Grosso, Brazil: Prevalence, Molecular Characterization and Resistance to Antibiotics and Disinfectants

et al. 2019

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“…Phenotypically, multiresistant isolates did not show antibiotic resistance genes in a study performed by Muñoz et al (). This fact suggests that other mechanisms may contribute to phenotypic resistance, or other genes that may be involved in the resistance acquisition mechanism (Haubert, Mendonça, & Lopes, ).…”

Section: Discussionmentioning

confidence: 99%

Probiotic potential ofLactobacillus curvatusP99 and viability in fermented oat dairy beverage

Funck

Marques

Cruxen

et al. 2019

J Food Process Preserv

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The aim of this study was to evaluate the probiotic potential and the viability in fermented oat dairy beverage of Lactobacillus curvatus isolate P99. Probiotic potential was assessed through the survival in simulated gastrointestinal tract conditions, autoaggregation, coaggregation, and hydrophobicity capacity and antimicrobial activity. The safety aspects of the isolate were evaluated for application in food, and these were determined by phenotypic and genotypic tests. Isolate P99 was able to survive in simulated gastrointestinal tract conditions and showed hydrophobicity, autoaggregation, and coaggregation with Listeria monocytogenes. Moreover, the isolate and its cell free supernatant showed antimicrobial activity against Gram‐positive and Gram‐negative microorganisms. L. curvatus P99 showed in vitro probiotic potential, fulfilled safety aspects, and remained viable for 35 days in fermented oat dairy beverage stored at 4°C. Moreover, probiotic fermented oat dairy beverage demonstrated high acceptability. Therefore, isolate P99 can be considered a promising microorganism for the manufacture of probiotic foods. Practical applications Probiotics are microorganisms capable of providing health benefits to the host. However, isolates with probiotic potential from foods, when used as adjuvant cultures in foods, promote desirable sensory attributes and ensure microbiologically safe food. In this study, we found that Lactobacillus curvatus P99 presented probiotic potential and remains viable for 35 days when used in the production of fermented oat dairy beverage. Therefore, isolate P99 can be considered a promising microorganism for the manufacture of probiotic foods.

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“…All isolates showed resistance to oxacillin while only 0.5% were resistant to tetracycline. Haubert, Mendonc, Lopes, de Itapema Cardoso, & da Silva () studied the antibiotic resistance of 50 L. monocytogenes strains isolated from foods and food environment in Brazil, between 2001 and 2010. They found that all isolates were resistant to nalidixic acid and cefoxitin.…”

Section: Prevalence Of Antibiotic Resistance Among Food Isolates Of Lmentioning

confidence: 99%

“…Similarly, the genes ermB , tetM , and dfrD , were detected in L. monocytogenes strains isolated from food and environmental samples in France during 1996 to 2006; but the tetS , tetK , and tetL genes were not detected (Granier et al., ). The antibiotic resistance genes tetM and ermB were also identified in L. monocytogenes isolated from fresh mixed sausage and chicken slaughterhouse, respectively (Haubert et al., ). Recently, Lim, Yap, and Thong () found that two L. monocytogenes isolates from fried fish and salad carried five genes including tetA , lmrB , mecC , msrA , and fosX that confer resistance to tetracycline, lincomycin, beta‐lactam, erythromycin, and fosfomycin, respectively.…”

Section: Mechanisms Of Antibiotic Resistance In L Monocytogenesmentioning

confidence: 99%

Emergence of Antibiotic Resistance in Listeria monocytogenes Isolated from Food Products: A Comprehensive Review

Olaimat

Al‐Holy

Shahbaz

et al. 2018

Comp Rev Food Sci Food Safe

188

120

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Listeria monocytogenes is an opportunistic pathogen that has been involved in several deadly illness outbreaks. Future outbreaks may be more difficult to manage because of the emergence of antibiotic resistance among L. monocytogenes strains isolated from food products. The present review summarizes the available evidence on the emergence of antibiotic resistance among L. monocytogenes strains isolated from food products and the possible ways this resistance has developed. Furthermore, the resistance of food L. monocytogenes isolates to antibiotics currently used in the treatment of human listeriosis such as penicillin, ampicillin, tetracycline, and gentamicin, has been documented. Acquisition of movable genetic elements is considered the major mechanism of antibiotic resistance development in L. monocytogenes. Efflux pumps have also been linked with resistance of L. monocytogenes to some antibiotics including fluoroquinolones. Some L. monocytogenes strains isolated from food products are intrinsically resistant to several antibiotics. However, factors in food processing chains and environments (from farm to table) including extensive or sub-inhibitory antibiotics use, horizontal gene transfer, exposure to environmental stresses, biofilm formation, and presence of persister cells play crucial roles in the development of antibiotic resistance by L. monocytogenes.

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Listeria monocytogenesisolates from food and food environment harbouringtetMandermBresistance genes

Cited by 52 publications

References 39 publications

Listeria monocytogenes in Export-approved Beef from Mato Grosso, Brazil: Prevalence, Molecular Characterization and Resistance to Antibiotics and Disinfectants

Listeria monocytogenes in Export-approved Beef from Mato Grosso, Brazil: Prevalence, Molecular Characterization and Resistance to Antibiotics and Disinfectants

Probiotic potential ofLactobacillus curvatusP99 and viability in fermented oat dairy beverage

Emergence of Antibiotic Resistance in Listeria monocytogenes Isolated from Food Products: A Comprehensive Review

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