<i>Lr72</i> Confers Resistance to Leaf Rust in Durum Wheat Cultivar Atil C2000

Herrera-Foessel, Sybil A.; Espino, Julio Huerta; Calvo-Salazar, Violeta; Lan, Caixia; Singh, Ravi P.

doi:10.1094/pdis-07-13-0741-re

Cited by 48 publications

(38 citation statements)

References 21 publications

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“…However, races of the leaf rust pathogen, virulent to widely grown durum cultivars in several production areas, are increasingly impacting durum production (Singh et al, 2004; Goyeau et al, 2006; Huerta‐Espino et al, 2009). A Pt race, BBG/BN, in northwestern Mexico that appeared in 2001, with virulence to Lr72 , overcame the resistance of widely adapted durum wheat cultivars from the CIMMYT breeding program that had been effective in Mexico for >25 yr (Singh et al, 2004; Herrera‐Foessel et al, 2014). Increased susceptibility of durum wheat to leaf rust has also been observed in the Mediterranean basin (Martinez et al, 2005; Goyeau et al, 2012), the Middle East (Ordoñez and Kolmer, 2007a), and Chile (Singh et al, 2004).…”

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confidence: 99%

Genome‐Wide Association Mapping of Leaf Rust Response in a Durum Wheat Worldwide Germplasm Collection

Aoun¹,

Breiland²,

Turner³

et al. 2016

The Plant Genome

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Leaf rust (caused by Puccinia triticina Erikss. [Pt]) is increasingly impacting durum wheat (Triticum turgidum L. var. durum) production with the recent appearance of races with virulence to widely grown cultivars in many durum producing areas worldwide. A highly virulent P. triticina race on durum wheat was recently detected in Kansas. This race may spread to the northern Great Plains, where most of the US durum wheat is produced. The objective of this study was to identify sources of resistance to several races from the United States and Mexico at seedling stage in the greenhouse and at adult stage in field experiments. Genome-wide association study (GWAS) was used to identify single-nucleotide polymorphism (SNP) markers associated with leaf rust response in a worldwide durum wheat collection of 496 accessions. Thirteen accessions were resistant across all experiments. Association mapping revealed 88 significant SNPs associated with leaf rust response. Of these, 33 SNPs were located on chromosomes 2A and 2B, and 55 SNPs were distributed across all other chromosomes except for 1B and 7B. Twenty markers were associated with leaf rust response at seedling stage, while 68 markers were associated with leaf rust response at adult plant stage. The current study identified a total of 14 previously uncharacterized loci associated with leaf rust response in durum wheat. The discovery of these loci through association mapping (AM) is a significant step in identifying useful sources of resistance that can be used to broaden the relatively narrow leaf rust resistance spectrum in durum wheat germplasm. Abbreviations: AM, association mapping; ARS, Agricultural Research Service; CDL, Cereal Disease Laboratory; GWAS, genome-wide association study; IT, infection type; LD, linkage disequilibrium; MAF, minor allele frequency; MR, moderately resistant; MS, moderately susceptible; MSD, mean squared difference; NSGC, National Small Grain Collection; PC, principal component; PCA, principal component analysis; pFDR, positive false discovery rate; Pt, Puccinia triticina Erikss.; QTL, quantitative trait loci; R, resistant; S, susceptible; SNP, single-nucleotide polymorphism; SSR, simple-sequence repeat. Core Ideas• Thirteen durum wheat accessions showed resistance to all Puccinia triticina races tested• GWAS revealed 88 SNPs (37 loci) associated with leaf rust response in durum wheat• Associations were identified on all chromosomes except 1B and 7B• GWAS revealed 14 previously uncharacterized loci for leaf rust resistance

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mentioning

confidence: 99%

Genome‐Wide Association Mapping of Leaf Rust Response in a Durum Wheat Worldwide Germplasm Collection

Aoun¹,

Breiland²,

Turner³

et al. 2016

The Plant Genome

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“…ʽAtil C2000' (Herrera‐Foessel et al. ). Trinakria showed seedling resistance to both pathotypes 12‐2 and 104‐2 (Gireesh et al.…”

Section: Discussionmentioning

confidence: 99%

“…Assuming that the genes for resistance to pathotypes 12-2 and 104-2 were the same, at least eight dominant genes were identified. Seventy-two leaf rust resistance genes have so far been catalogued (McIntosh et al 2013, Herrera-Foessel et al 2014 Trinakria showed seedling resistance to both pathotypes 12-2 and 104-2 (Gireesh et al 2014), but 'Wollaroi' and lines carrying Lr61 or Lr72 have not been tested with Indian pathotypes; therefore, their identities relative to the currently identified genes are unknown. It seems that a combination of Lr14a and Lr72 provides resistance in India (Loladze et al 2014), because there is no published report of combined virulence for these genes (Loladze, pers.…”

Section: Leaf Rust Resistancementioning

confidence: 99%

Sources of resistance to Indian pathotypes of Puccinia graminis tritici and P. triticina in durum wheat

et al. 2015

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Stem rust and leaf rust are important diseases affecting durum wheat production in India. Inheritance and extent of diversity in resistance were studied in five durum genotypes, viz. ʽB 662', ʽED 2398-A', ʽHG 110', ʽIWP 5019' and ʽLine 1172ʼ using Pgt pathotypes 40A and 117-6, and Pt pathotypes 12-2 and 104-2. F 2 and F 3 analyses showed that resistance was conferred by one or two genes in each line. In all, four genes for resistance to 40A, and eight each for pathotypes 117-6 and 12-2 were identified among the five genotypes, and three for resistance to 104-2 among B 662, ED 2398-A and IWP 5019 were indicated by tests of allelism. Although the gene identities are not known, at least some of them should be different from Sr2, Sr7b, Sr8a, Sr8b, Sr9e, Sr9g, Sr11, Sr12, Sr13, Sr14, Sr17, Sr23 and Sr28; and from Lr3, Lr14a, Lr23 and Lr27 + Lr31. These genotypes enrich the diversity of resistance to stem rust and leaf rust for durum wheat improvement.Key words: genetic diversity -Lr genes -resistance durability -Sr genes -Triticum turgidum ssp. durum India produces more than 90 million tonnes of wheat from an area of more than 25 million hectares, to which the contribution of durum wheat is about 5%. However, durum wheat has a special niche in the Indian wheat economy for at least two reasons: firstly, Indian durum is typically purchased by private traders at a premium price, mainly for processing high-value products; secondly, durum is preferred over bread wheat for several local food preparations. Durum and cultivated emmer are mainly grown in the central and southern parts, where leaf rust and stem rust are the major disease problems affecting wheat. Broadening the resistance base through the utilization of genetically diverse resistance sources is necessary to enhance the durability of resistance to meet ongoing threats from the continuously evolving rust pathogens. Relatively, little work has been done on the inheritance of rust resistance in durum, compared to bread wheat. However, studies conducted in different parts of the world indicate that durum generally shows resistance to Puccinia triticina (Pt) pathotypes virulent to bread wheat and vice versa

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“…Selection responses in MAS breeding programmes are higher as molecular markers can be used for selection even at the seedling stage, with high precision and in a non‐target environment (Collard & Mackill, ). Recently, several genes and QTLs governing rust resistances have been reported in wheat (Buerstmayr et al ., ; Herrera‐Foessel et al ., , ). Since then, single nucleotide polymorphism (SNP) markers were developed for genetic mapping of the SrCad gene conferring resistance to Ug99 stem rust and diagnostic SNP markers were also produced for the wheat leaf rust resistance gene Lr16 in wheat (Kassa et al ., , ).…”

Section: Modern Molecular Breeding Toolsmentioning

confidence: 99%

Molecular breeding technologies and strategies for rust resistance in wheat (Triticum aestivum) for sustained food security

et al. 2017

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Wheat is an important cereal food crop providing key nutrients to humankind. Rusts are the most destructive pathogens of cereal crops, with the exception of rice, across the world and resistant cultivars have been widely employed to reduce the yield losses caused by them. The modern intensive monoculture of cultivars and changing climatic conditions has created congenial conditions for the emergence of new virulent races such as Ug99, which is a great concern for world food security. Conventional breeding efforts have not been effective in quickly developing new varieties with durable and broad‐spectrum resistance against the rapidly evolving rust pathogen races. However, in the last two decades, biotechnological methods such as marker‐assisted selection (MAS) and transgenic technology have provided novel strategies for enhancing resistance levels and durability in crop plants in a short span of time. Nevertheless, broad application of transgenics in agriculture is hindered by biosafety apprehensions. In recent years, improved versions of biotechnological breeding methods such as genomic selection, genome editing technologies, cisgenesis and intragenesis, RNA‐dependent DNA methylation (RdDM), agroinfiltration and reverse breeding are gaining popularity. These technologies provide a tremendous capability to manipulate crop plants more precisely than before and accelerate crop improvement efforts for sustained food production as well as overcoming safety concerns associated with food crops.

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Lr72 Confers Resistance to Leaf Rust in Durum Wheat Cultivar Atil C2000

Abstract: Herrera-Foessel, S. A., Huerta-Espino, J., Calvo-

Cited by 48 publications

References 21 publications

Genome‐Wide Association Mapping of Leaf Rust Response in a Durum Wheat Worldwide Germplasm Collection

Genome‐Wide Association Mapping of Leaf Rust Response in a Durum Wheat Worldwide Germplasm Collection

Sources of resistance to Indian pathotypes of Puccinia graminis tritici and P. triticina in durum wheat

Molecular breeding technologies and strategies for rust resistance in wheat (Triticum aestivum) for sustained food security

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