Lumbricus rubellus Protein Fraction DLBS1033N Increases Nerve Growth Factor Expression via Tyrosine Kinase Activation

Abstract

Nerve injury occurred due to mechanical, thermal, chemical, or ischemic factors. Nerve regeneration is needed for recovery. Schwann cell proliferation and differentiation are important factors in the nerve regeneration process. Schwann cells release neurotrophins in the nerve regeneration process. In this study, Lumbricus rubellus protein fraction DLBS1033N was used as therapeutic protein candidate for nerve regeneration treatments. DLBS1033N treatments promoted the growth and survival of Schwann cell in free serum and free serum plus minimum O 2 conditions. Real-time PCR and ELISA methods revealed that DLBS1033N induced NGF expressions. The growth and survival of Schwann cells were related to NGF expressions in a specific inhibitor TrkA study. Furthermore, in real-time PCR study, DLBS1033N was able to activate phosphatidylinositol-3-kinase (PI3K) pathway. This study showed that L. rubellus protein fraction DLS1033N can promote the growth and survival of Schwann cells by inducing NGF expressions. Cells growth and survival activities are likely achieved via PI3K pathway.

Enhancement Peripheral Regeneration as a Target of Potential Diabetic Neuropathy Treatment from Lumbricus rubellus Fraction DLBS1033N: the role of cell viability and migration

“…DLBS1033N was confirmed to promote RSC96 growth and survival in the hypoxia-ischemic model through PI3k/Akt signaling activation. Moreover, NGF expression, an essential neurotrophic factor in the regeneration process, was significantly increased by DLBS1033N (Karsono et al, 2018).…”

“…DLBS1033N was prepared as mentioned in the previous experiment (Karsono et al, 2018). The total protein content of DLBS1033N was estimated using Bradford Assay.…”

“…Thus, 50 mM glucose concentration was deemed appropriate for future experiments. The protection effect of DLB1033N at concentrations 25, 50, and 100 μg/mL (Karsono et al, 2018) was determined with the presence of 50 mM glucose.…”

“…DLBS1033N mechanism was confirmed to promote cell viability in high-glucose conditions and has potential to induce cell migration. Radical scavenging by phenolic compound (Aldarraji, 2013) and induction of NGF secretion (Karsono et al 2018) contribute to DLBS1033N potential therapy of DPN.…”

“…A previous study confirmed DLBS1033N activity as a therapeutic candidate for nerve regeneration treatments by promoting Schwann cell line RSC96 growth and survival. This mechanism of action involves the induction NGF expression through PI3K signaling pathway interaction (Karsono et al, 2018). Due to the depletion of NGF expression in DPN, it is critical to carry out a study investigating the effect of DLBS1033N linking the aspect of diabetic degeneration with induction of NGF expression.…”