<i>Lycium barbarum</i> polysaccharide‐glycoprotein promotes osteogenesis in hPDLSCs via ERK activation

Lai, Shuang; Liu, Cong; Fan, Liyuan; Deng, Li; Yang, Yiling; Liu, Chang; Zhu, Yushu; Li, Xinlun; Li, Xiao; Mu, Yandong

doi:10.1111/odi.14409

Cited by 6 publications

(3 citation statements)

References 47 publications

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“…The study also showed a reduction in alveolar bone resorption and confirmed the positive effect of L. barbarum for managing periodontitis. 65 Hence based on our results and existing evidence, it can be stated that goji berry has a promising role inmanaging periodontitis. However, one should also note that the present study evaluated the role of goji berry for only a short recall time for one month and prolonged exposure to chlorhexidine mouthwash was avoided to prevent side effects.…”

Section: Discussionsupporting

confidence: 52%

Efficacy of Lycium barbarum (Goji berry) mouthwash for managing periodontitis: a randomized clinical trial

Sanghavi,

Shettigar,

Chopra

et al. 2023

F1000Res

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Background Removal of the microbial deposits (plaque and calculus) by performing effective scaling and root planing (SRP) is the fundamental step for managing periodontal disease (gingivitis and periodontitis). Various adjuncts in the form of mouthwash, gels, and toothpaste are also being used as adjuncts to SRP for managing periodontitis. Recently, Lycium barbarum (L. barbarum), commonly known as goji berry or wolfberry, has gained popularity for managing chronic inflammatory and infectious diseases. However, its efficacy in managing periodontitis has never been explored. Hence the present study aims to evaluate the efficacy of goji berry mouthwash compared to chlorhexidine mouthwash for managing periodontitis. Methods 60 adult participants were divided randomly using computer-generated random sequences into two groups (case group: L. barbarum mouthwash (Males: 16; Female: 14); control group: 0.2% chlorhexidine gluconate mouthwash (Males: 14; Females: 16)). The changes in the gingival index (Gi), plaque index (Pi), bleeding on probing (BOP), clinical attachment loss (CAL), probing pocket depth (PPD), microbial load, and antioxidant levels (protein thiol) in saliva were noted at the baseline, at 15 days and one month. Results A significant reduction in the mean PPD, Pi, and Gi was seen from baseline to one month in both the control (P-value=0.006, 0.027, and 0.036 respectively) and test groups (P-value=0.035, 0.000, and 0.000 respectively). CAL was reduced significantly only in the control group. However, the antioxidant levels (protein thiol) in saliva were significantly increased only in the test group. Conclusion Goji berry mouthwash along with SRP reduced the Gi, Pi, BOP, and PPD in patients with periodontitis. However, no statistically significant difference was noted between the use of goji berry and chlorhexidine mouthwash. Goji berry mouthwash was more effective than chlorhexidine in increasing the antioxidant levels in saliva.

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Section: Discussionsupporting

confidence: 52%

Efficacy of Lycium barbarum (Goji berry) mouthwash for managing periodontitis: a randomized clinical trial

Sanghavi,

Shettigar,

Chopra

et al. 2023

F1000Res

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“…Numerous studies have employed hPDLSCs in both in vivo and in vitro repair and regeneration experiments, demonstrating their effectiveness in promoting osteogenesis (24)(25)(26). However, the complexity of material construction has hindered their widespread adoption.…”

Section: Discussionmentioning

confidence: 99%

Naringenin modulates the NO‑cGMP‑PKG signaling pathway by binding to AKT to enhance osteogenic differentiation in hPDLSCs

Li,

Xiong,

Lan

et al. 2024

Int J Mol Med

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Naringenin (NAR) is a prominent flavanone that has been recognized for its capacity to promote the osteogenic differentiation of human periodontal ligament stem cells (hPDLSCs). The present study aimed to explore how NAR promotes the osteogenic differentiation of hPDLSCs and to assess its efficacy in repairing alveolar bone defects. For this purpose, a protein-protein interaction network of NAR action was established by mRNA sequencing and network pharmacological analysis. Gene and protein expression levels were evaluated by reverse transcription-quantitative and western blotting. Alizarin red and alkaline phosphatase staining were also employed to observe the osteogenic capacity of hPDLSCs, and immunofluorescence was used to examine the co-localization of NAR molecular probes and AKT in cells. The repair of mandibular defects was assessed by micro-computed tomography (micro-CT), Masson staining and immunofluorescence. Additionally, computer simulation docking software was utilized to determine the binding affinity of NAR to the target protein, AKT. The results demonstrated that activation of the nitric oxide (NO)-cyclic guanosine monophosphate (cGMP)-protein kinase G (PKG) signaling pathway could promote the osteogenic differentiation of hPDLSCs. Inhibition of AKT, endothelial nitric oxide synthase and soluble guanylate cyclase individually attenuated the ability of NAR to promote the osteogenic differentiation of hPDLSCs. Micro-CT and Masson staining revealed that the NAR gavage group exhibited more new bone formation at the defect site. Immunofluorescence assays confirmed the upregulated expression of Runt-related transcription factor 2 and osteopontin in the NAR gavage group. In conclusion, the results of the present study suggested that NAR promotes the osteogenic differentiation of hPDLSCs by activating the NO-cGMP-PKG signaling pathway through its binding to AKT.

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“…To our knowledge, there has been no data in the literature comparing salivary Thy‐1 in periodontitis and obesity. Thy‐1, also known as CD90, is expressed on fibroblasts, rodent thymocytes, and T cells (Rege & Hagood, 2006), and various types of MSCs including bone marrow, periodontal ligament, adipose tissue, lung, liver, placenta, spleen (Calloni et al, 2013; Lai et al, 2022; Ying & Zhang, 2023), and inflamed tissue (Liao et al, 2011). Thy‐1 is evidenced in parotid, labial, and minor salivary glands (Andreadis et al, 2014; Togarrati et al, 2018; Wang et al, 2017; Yi et al, 2016).…”

Section: Discussionmentioning

confidence: 99%

Evaluation of salivary Thy‐1 in health, periodontitis, and obesity

et al. 2023

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ObjectivesThy‐1 (CD90) is a glycosylphosphatidyl‐anchored protein belonging to the immunoglobulin family and is known to control mesenchymal stromal cells differentiation into osteoblasts or adipocytes. The study aimed to investigate the salivary levels of Thy‐1 in health, periodontitis, obesity, and any potential association.Materials and MethodsSeventy‐one participants were divided into four groups: healthy (H), subjects with periodontitis (P), obese individuals (O), and obese individuals having periodontitis (PO). Unstimulated whole saliva was collected from participants who were evaluated for periodontal parameters. The levels of Thy‐1 were measured with a commercially available ELISA kit. The data were statistically analyzed.ResultsA significant difference in salivary Thy‐1 levels among different groups was observed. Periodontitis patients had the maximum, and obese individuals had the minimum Thy‐1 levels. Significant differences between H and P, H and PO, P and O, and O and PO were observed. Overall correlations between Thy‐1 and periodontal parameters and a positive correlation with pocket depth in group PO were noted.ConclusionThy‐1 could be detected in the saliva of all study participants. It is implied that a local inflammatory condition like periodontitis elevates the salivary levels of Thy‐1 with, and without obesity.

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Lycium barbarum polysaccharide‐glycoprotein promotes osteogenesis in hPDLSCs via ERK activation

Cited by 6 publications

References 47 publications

Efficacy of Lycium barbarum (Goji berry) mouthwash for managing periodontitis: a randomized clinical trial

Efficacy of Lycium barbarum (Goji berry) mouthwash for managing periodontitis: a randomized clinical trial

Naringenin modulates the NO‑cGMP‑PKG signaling pathway by binding to AKT to enhance osteogenic differentiation in hPDLSCs

Evaluation of salivary Thy‐1 in health, periodontitis, and obesity

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