<i>Lymantria dispar</i> sex pheromone is a behavioral antagonist to pheromonal attraction of male <i>Lymantria mathura</i>

Gries, Regine; Schaefer, Paul W.; Nakamura, Kenichiro

doi:10.4039/n08-069

Cited by 5 publications

(4 citation statements)

References 8 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…But these four chemicals had different binding affinity with PBPs, and PBP genes did not map to a quantitative trait locus for response to pheromone 37 . And therefore we can not classify them as agonists or antagonists of pheromone 38 , 39 , like (+)-D being a behavioral antagonists to pheromonal attraction of male L. mathura 40 . However, the different affinity of PBPs to chemicals showed that PBPs can recognize and decode the chemicals, and these chemicals did influence the behavior, but such result need to be confirmed in field experiments.…”

Section: Discussionmentioning

confidence: 99%

Structural and Functional Difference of Pheromone Binding Proteins in Discriminating Chemicals in the Gypsy Moth, Lymantria Dispar

Ma²,

Yi-xia³

et al. 2012

Int. J. Biol. Sci.

View full text Add to dashboard Cite

Pheromone-binding proteins (PBPs) of the gypsy moth, Lymantria dispar L., play an important role in olfaction. Here structures of PBPs were first built by Homology Modeling, and each model of PBPs had seven α-helices and a large hydrophobic cavity including 25 residues for PBP1 and 30 residues for PBP2. Three potential semiochemicals were first screened by CDOCKER program based on the PBP models and chemical database. These chemicals were Palmitic acid n-butyl ester (Pal), Bis(3,4-epoxycyclohexylmethyl) adipate (Bis), L-trans-epoxysuccinyl-isoleucyl-proline methyl ester propylamide (CA-074). The analysis of chemicals docking the proteins showed one hydrogen bond was established between the residues Lys94 and (+)-Disparlure ((+)-D), and л-л interactions were present between Phe36 of PBP1 and (+)-D. The Lys94 of PBP1 formed two and three hydrogen bonds with Bis and CA-074, respectively. There was no residue of PBP2 interacting with these four chemicals except Bis forming one hydrogen bond with Lys121. After simulating the conformational changes of LdisPBPs at pH7.3 and 5.5 by constant pH molecular dynamics simulation in implicit solvent, the N-terminal sequences of PBPs was unfolded, only having five α-helices, and PBP2 had larger binding pocket at 7.3 than PBP1. To investigate the changes of α-helices at different pH, far-UV and near-UV circular dichroism showed PBPs consist of α-helices, and the tertiary structures of PBP1 and PBP2 were influenced at pH7.3 and 5.5. The fluorescence binding assay indicated that PBP1 and PBP2 have similarly binding affinity to (+)-D at pH 5.5 and 7.3, respectively. At pH 5.5, the dissociation constant of the complex between PBP1 and 2-decyl-1-oxaspiro [2.2] pentane (OXP1) was 0.68±0.01μM, for (+)-D was 5.32±0.11μM, while PBP2 with OXP1 and (+)-D were 1.88±0.02μM and 5.54±0.04μM, respectively. Three chemicals screened had higher affinity to PBP1 than (+)-D except Pal at pH5.5, and had lower affinity than (+)-D at pH7.3. To PBP2, these chemicals had lower affinity than the sex pheromone except Bis at pH 5.5 and pH 7.3. Only PBP1 had higher affinity with Sal than the sex pheromone at pH 5.5. Therefore, the structures of PBP1 and PBP2 had different changes at pH5.5 and 7.3, showing different affinity to chemicals. This study helps understanding the role of PBPs as well as in developing more efficient chemicals for pest control.

show abstract

Section: Discussionmentioning

confidence: 99%

Structural and Functional Difference of Pheromone Binding Proteins in Discriminating Chemicals in the Gypsy Moth, Lymantria Dispar

Ma²,

Yi-xia³

et al. 2012

Int. J. Biol. Sci.

View full text Add to dashboard Cite

show abstract

“…That the L. mathura pheromone components (+)and (-)-mathuralure, singly (Fig. 2) and in combination (Gries et al 2009), had no antagonistic effect on L. d. japonica is not understood because (+)-disparlure, in turn, is a behavioural antagonist to pheromonal communication by L. mathura (Gries et al 2009).…”

Section: Resultsmentioning

confidence: 99%

“…For efficient pheromone-based detection surveys of exotic species of Lymantria Hübner, lures for several species should be deployed. There is evidence, however, that components of the pheromone/volatile blend from one species can be antagonistic to that of another (Grant et al 1996;Gries et al 1996Gries et al , 2009. Thus, for optimal attractiveness, synthetic pheromone lures used in detection surveys require sufficient spacing.…”

Section: Introductionmentioning

confidence: 99%

Spacing of traps baited with species-specific Lymantria pheromones to prevent interference by antagonistic components

et al. 2009

Self Cite

View full text Add to dashboard Cite

In pheromone-based surveys for detecting multiple species of exotic lymantriine moths (Lepidoptera: Noctuidae: Lymantriinae), spacing between traps baited with species-specific pheromone lures must be sufficient to prevent antagonistic effects of heterospecific pheromone on lure attractiveness. Conducting field experiments with the Japanese gypsy moth, Lymantria dispar japonica Motschulsky, in northern Honshu, Japan, we first determined which congeneric pheromone components have strong antagonistic effects on attraction of male moths to the conspecific pheromone (7R,8S)-cis-7,8-epoxy-2-methyloctadecane ((+)-disparlure). Since the most antagonistic compounds were pheromone/volatile components from the sympatric nun moth, L. monacha (L.), we then conducted experiments with paired traps baited with either a L. dispar (L.) pheromone lure ((+)-disparlure (50 µg)) or L. monacha pheromone lure (a mixture of (7R,8S)-cis-7,8-epoxyoctadecane ((+)-monachalure (50 µg)), (7Z)-2-methyloctadecene (5 µg), and (+)-disparlure (50 µg)). As spacing between paired traps increased (0, 0.5, 2, 7.5, 15, or 30 m), the antagonistic effect of the L. monacha lure on the attractiveness of the L. dispar lure decreased and finally disappeared. For pheromone-based detection surveys of multiple species of exotic lymantriine moths in North America to be effective, trap spacing of 15 m is recommended.

show abstract

“…Our electrophysiological results alongside with the studies mentioned above on other species of tineids/sesiids suggest that the E 3 Z 13‐18:OH may be a putative pheromone antagonist. Closely related moth species, as well as phylogenetically distant species with completely different pheromone components can be antagonized by pheromone components from other lepidopterans living in the same habitat (Gries et al, 2009; Mozūraitis et al, 2006; Wang, Shen, et al, 2022). Pheromone antagonists have the great potential as an alternative material in integrated pest management strategies (Renou & Guerrero, 2000).…”

Section: Discussionmentioning

confidence: 99%

Stored product pest Nemapogon variatella (Clemens): Identification of sex pheromone, putative antagonist and mitotype characterization

Radžiutė,

Orlovskytė,

Būdienė

et al. 2023

J Applied Entomology

View full text Add to dashboard Cite

Nemapogon variatella (Clemens) (Lepidoptera: Tineidae) is a worldwide widespread stored product pest that damages grain, dried fruits and mushrooms. The most powerful and environmentally friendly tool for insect pest monitoring and control is pheromone application. In our study, we revealed for the first time that the females of N. variatella release (3Z,13Z)‐octadecadien‐1‐ol – a single compound that activates olfactory receptors in the antenna of conspecific males. These data allow to assign this compound, which has been known as an attractant for males only, to the category of sex pheromones of N. variatella moths. Males' olfactory system is sensitive not only to the sex pheromone compound but also to (3E,13Z)‐octadecadien‐1‐ol as well. (3E,13Z)‐octadecadien‐1‐ol is attributed to putative pheromone antagonists. During the analysis of genetic diversity, six mitotypes of N. variatella CO1‐5′ were detected and preliminary named Mit1–Mit6. The obtained high haplotype diversity (Hd = 0.796) and low nucleotide diversity (Pi = 0.007) suggest that this species may have undergone a recent population expansion.

show abstract

Lymantria dispar sex pheromone is a behavioral antagonist to pheromonal attraction of male Lymantria mathura

Cited by 5 publications

References 8 publications

Structural and Functional Difference of Pheromone Binding Proteins in Discriminating Chemicals in the Gypsy Moth, Lymantria Dispar

Structural and Functional Difference of Pheromone Binding Proteins in Discriminating Chemicals in the Gypsy Moth, Lymantria Dispar

Spacing of traps baited with species-specific Lymantria pheromones to prevent interference by antagonistic components

Stored product pest Nemapogon variatella (Clemens): Identification of sex pheromone, putative antagonist and mitotype characterization

Contact Info

Product

Resources

About