Mr‐values of mature subunits I and III of beef heart cytochrome c oxidase in relationship to nucleotide sequences of their genes

Darley‐Usmar, Victor; Fuller, Stephen D.

doi:10.1016/0014-5793(81)80968-4

Cited by 15 publications

(2 citation statements)

References 11 publications

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“…The simplest explanation for the discrepancy between the estimated and derived molecular weights is that molecular weight estimations of highly hydrophobic proteins based on electrophoretic mobility in sodium dodecyl sulphate-polyacrylamide gels are frequently unreliable (35,36) and it is interesting to note that the difference between the estimated molecular weights (44 kd and 51 kd) of the two photosystem 11 reaction-centre proteins is almost the same as that between their derived molecular weights (51816 and 56246) (this paper and ref. 17).…”

Section: Discussionmentioning

confidence: 80%

Structure of the spinach chloroplast genes for the D2 and 44 kd reaction-centre proteins of pbotosystem II and for tRNA^Ser(UGA)

1984

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We have determined the sequence of the spinach (Spinacia oleracea) chloroplast genes for the photosystem II proteins, D2 and the 44 kd reaction-centre, chlorophyll a-binding protein, and for tRNASer (UGA). The 3' end of the D2 gene overlaps the first 50 bp of the 5' end of the gene for the 44 kd protein. Northern RNA hybridization analysis indicates the two genes are cotranscribed into a single 3.5 kb RNA. The predicted molecular weight of the 353-residue D2 protein is 39536 and that of the 473-residue 44 kd protein is 51816. Both proteins are hydrophobic containing at least five possible membrane-spanning domains. D2 shows significant homology to the 32 kd herbicide-binding protein (Zurawski et al., (1982) Proc. Natl. Acad. Sci. USA 79, 7699-7703), and parts of the 44 kd protein show obvious similarities to parts of the 51 kd reaction-centre, chlorophyll a-binding protein of photosystem II (Morris and Herrmann (1984) Nucleic Acids Res. 12, 2837-2850). The gene for tRNASer (UGA) which is on the opposite strand to and transcribed towards the photosystem II genes is 72% homologous with the corresponding Escherichia coli tRNASer.

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Section: Discussionmentioning

confidence: 80%

Structure of the spinach chloroplast genes for the D2 and 44 kd reaction-centre proteins of pbotosystem II and for tRNA^Ser(UGA)

1984

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“…wt. of hydrophobic, membrane proteins which are known to migrate anomalously in various gel systems (Darley-Usmar and Fuller, 1981;Williams et al, 1983;Youvan et al, 1984), and DNA sequence analysis shows the presence of two ATGs which may serve as initiation codons. We have indicated (Figure 3) the sequence for the largest, 38.95-kd protein coded for by psbA, as was given for spinach and N. debneyi (Zurawski et al, 1982).…”

Section: Intronsmentioning

confidence: 99%

Chlamydomonas reinhardii gene for the 32 000 mol. wt. protein of photosystem II contains four large introns and is located entirely within the chloroplast inverted repeat

1984

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The chloroplast psbA gene from the green unicellular alga Chlamydomonas reinhardii has been localized, cloned and sequenced. This gene codes for the rapidly‐labeled 32‐kd protein of photosystem II, also identified as as herbicide‐binding protein. Unlike psbA in higher plants which is found in the large single copy region of the chloroplast genome and is uninterrupted, psbA in C. reinhardii is located entirely within the inverted repeat, hence present in two identical copies per circular chloroplast genome, and contains four large introns. These introns range from 1.1 to 1.8 kb in size and fall into the category of Group I introns. Two of the introns contain open reading frames which are in‐frame with the preceding exon sequences. We present the nucleotide sequence for the C. reinhardii psbA 5′‐and 3′ ‐flanking sequences, the coding region contained in five exons and the deduced amino acid sequence. The algal gene codes for a protein of 352 amino acid residues which is 95% homologous, excluding the last eight amino acid residues, with the higher plant protein.

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Nucleotide sequence of the clustered genes for the 44 kd chlorophyll a apoprotein and the ?32 kd?-like protein of the photosystem II reaction center in the spinach plastid chromosome

et al. 1984

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A 2,900 base pair DNA segment of the spinach plastid chromosome which encodes the genes for the 44 kd chlorophyll a apoprotein and a "32 kd"-like protein of the photosystem II reaction center has been subjected to sequence and Northern blot analysis. The genes are located almost centrally in the large single-copy segment of the chromosome adjacent to the two genes for the P700 chlorophyll a apoproteins of the photosystem I reaction center. The DNA sequence reveals two uninterrupted protein-coding regions of 473 (44 kd chlorophyll a apoprotein) and 353 triplets ("32 kd"-like protein). The latter gene is strikingly similar to the gene for the herbicide-binding "32 kd" protein which maps some 30 kbp distant on the plastid chromosome. The two genes overlap by 50 base pairs but are read in different phases. They may be contranscribed and the RNA modified to give several discrete species ranging in size from 1.6 to 4.6 kb. A presumptive promoter site was only identified for the "32 kd"-like protein, while potential ribosome binding and transcription termination sites are found preceding and following both genes, respectively. The polypeptides possess a high content of hydrophobic amino acids, most of which appear to be clustered in transmembrane spans. The molecular weights of 51,785 (44 kd chlorophyll a apoprotein) and 39,465 ("32 kd"-like protein) derived from the deduced amino acid sequences are higher than the experimentally determined protein sizes. Amino acid codon usage for both genes is highly selective. Comparison of the chlorophyll a apoproteins of spinach reveals regions of sequence homology.

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M_r‐values of mature subunits I and III of beef heart cytochrome c oxidase in relationship to nucleotide sequences of their genes

Cited by 15 publications

References 11 publications

Structure of the spinach chloroplast genes for the D2 and 44 kd reaction-centre proteins of pbotosystem II and for tRNA^Ser(UGA)

Structure of the spinach chloroplast genes for the D2 and 44 kd reaction-centre proteins of pbotosystem II and for tRNA^Ser(UGA)

Chlamydomonas reinhardii gene for the 32 000 mol. wt. protein of photosystem II contains four large introns and is located entirely within the chloroplast inverted repeat

Nucleotide sequence of the clustered genes for the 44 kd chlorophyll a apoprotein and the ?32 kd?-like protein of the photosystem II reaction center in the spinach plastid chromosome

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Mr‐values of mature subunits I and III of beef heart cytochrome c oxidase in relationship to nucleotide sequences of their genes

Cited by 15 publications

References 11 publications

Structure of the spinach chloroplast genes for the D2 and 44 kd reaction-centre proteins of pbotosystem II and for tRNASer(UGA)

Structure of the spinach chloroplast genes for the D2 and 44 kd reaction-centre proteins of pbotosystem II and for tRNASer(UGA)

Chlamydomonas reinhardii gene for the 32 000 mol. wt. protein of photosystem II contains four large introns and is located entirely within the chloroplast inverted repeat

Nucleotide sequence of the clustered genes for the 44 kd chlorophyll a apoprotein and the ?32 kd?-like protein of the photosystem II reaction center in the spinach plastid chromosome

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M_r‐values of mature subunits I and III of beef heart cytochrome c oxidase in relationship to nucleotide sequences of their genes

Structure of the spinach chloroplast genes for the D2 and 44 kd reaction-centre proteins of pbotosystem II and for tRNA^Ser(UGA)

Structure of the spinach chloroplast genes for the D2 and 44 kd reaction-centre proteins of pbotosystem II and for tRNA^Ser(UGA)