<i>M. tuberculosis</i>‐Complex Specific T‐Cell Stimulation and DTH Reactions Induced With a Peptide from the 38‐kDa Protein

Vordermeier, H. M.; Harris, David P.; Mehrotra, P.K.; Román, Eulogia; Elsaghier, A.; Moreno, Carlos; Iványi, J

doi:10.1111/j.1365-3083.1992.tb02979.x

Cited by 30 publications

(19 citation statements)

References 37 publications

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“…As a surrogate marker for some of the key events involved in the expression of this type of protective immunity, the DTH reaction in the skin is often investigated (27). The absence of E-and P-selectin ligands on Th1 and Tc1 lymphocytes was previously demonstrated to annul trafficking of these T cells to inflamed cutaneous sites, as demonstrated by the loss of cutaneous hypersensitivity against dinitrofluorobenzene (14).…”

Section: Discussionmentioning

confidence: 99%

Selectin Ligand-Independent Priming and Maintenance of T Cell Immunity during Airborne Tuberculosis

Schreiber

Ehlers

Aly

et al. 2006

The Journal of Immunology

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Immunity to Mycobacterium tuberculosis infection is critically dependent on the timely priming of T effector lymphocytes and their efficient recruitment to the site of mycobacterial implantation in the lung. E-, P-, and L-selectin counterreceptors control lymphocyte homing to lymph nodes and leukocyte trafficking to peripheral sites of acute inflammation, their adhesive function depending on fucosylation by fucosyltransferases (FucT) IV and VII. To address the relative importance of differentially glycosylated selectin counterreceptors for priming of T cell effector functions in a model of mycobacteria-induced granulomatous pulmonary inflammation, we used aerosol-borne M. tuberculosis to infect FucT-IV−/−, FucT-VII−/−, FucT-IV−/−/FucT-VII−/−, or wild-type control mice. In lymph nodes, infected FucT-IV−/−/FucT-VII−/− and, to a lesser extent, FucT-VII−/− mice had severely reduced numbers of T cells and reduced Ag-specific effector responses. By contrast, recruitment of activated T cells into the lungs was similar in all four groups of mice during infection and expression of T cell, and macrophage effector functions were only delayed in lungs of FucT-IV−/−/FucT-VII−/− mice. Importantly, lungs from all groups expressed CXCL13, CCL21, and CCL19 and displayed organized follicular neolymphoid structures after infection with M. tuberculosis, which suggests that the lung served as a selectin ligand-independent priming site for immune responses to mycobacterial infection. All FucT-deficient strains were fully capable of restricting M. tuberculosis growth in infected organs until at least 150 days postinfection. Our observations indicate that leukocyte recruitment functions dictated by FucT-IV and FucT-VII-dependent selectin ligand activities are not critical for inducing or maintaining T cell effector responses at levels necessary to control pulmonary tuberculosis.

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Section: Discussionmentioning

confidence: 99%

Selectin Ligand-Independent Priming and Maintenance of T Cell Immunity during Airborne Tuberculosis

Schreiber

Ehlers

Aly

et al. 2006

The Journal of Immunology

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“…2 In this study, we transduced the murine melanoma cell line B16-B78 with a Mycobacterium tuberculosis (strain H37Rv) gene encoding a 38-kDa protein (Ag38), 9 which is one of the most immunogenic of the Mycobacterium tuberculosis antigens. 10 Transduced cells were injected into mice and the immune response against parental B16-B78 melanoma cells was evaluated.…”

Section: Introductionmentioning

confidence: 99%

Anti-tumor immunity induced by murine melanoma cells transduced with the Mycobacterium tuberculosis gene encoding the 38-kDa antigen

et al. 1998

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“…This indicates that the recombinant 27-kDa antigen is well recognized by the cellular immune response in mice during an experimental BGC infection. It might be of interest to test in the future whether this antigen can be used as a substitute of PPD to detect TB or contact patients or animals, as has been proposed for the 38-kDa and DPPD antigens (12,40).…”

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confidence: 99%

TheMycobacterium tuberculosisRecombinant 27-Kilodalton Lipoprotein Induces a Strong Th1-Type Immune Response Deleterious to Protection

et al. 2003

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Th1 immune response is essential in the protection against mycobacterial intracellular pathogens. Lipoproteins trigger both humoral and cellular immune responses and may be candidate protective antigens. We studied in BALB/c mice the immunogenicity and the protection offered by the recombinant 27-kDa Mycobacterium tuberculosis lipoprotein and the corresponding DNA vaccine. Immunization with the 27-kDa antigen resulted in high titers of immunoglobulin G1 (IgG1) and IgG2a with a typical Th1 profile and a strong delayed hypersensitivity response. A strong proliferation response was observed in splenocytes, and significant nitric oxide production and gamma interferon secretion but not interleukin 10 secretion were measured. Based on these criteria, the 27-kDa antigen induced a typical Th1-type immune response thought to be necessary for protection. Surprisingly, in 27-kDa-vaccinated mice (protein or DNA vaccines) challenged by M. tuberculosis H37Rv or BCG strains, there was a significant increase in the numbers of CFU in the spleen compared to that for control groups. Furthermore, the protection provided by BCG or other mycobacterial antigens was completely abolished once the 27-kDa antigen was added to the vaccine preparations. This study indicates that the 27-kDa antigen has an adverse effect on the protection afforded by recognized vaccines. We are currently studying how the 27-kDa antigen modulates the mouse immune response.

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M. tuberculosis‐Complex Specific T‐Cell Stimulation and DTH Reactions Induced With a Peptide from the 38‐kDa Protein

Cited by 30 publications

References 37 publications

Selectin Ligand-Independent Priming and Maintenance of T Cell Immunity during Airborne Tuberculosis

Selectin Ligand-Independent Priming and Maintenance of T Cell Immunity during Airborne Tuberculosis

Anti-tumor immunity induced by murine melanoma cells transduced with the Mycobacterium tuberculosis gene encoding the 38-kDa antigen

TheMycobacterium tuberculosisRecombinant 27-Kilodalton Lipoprotein Induces a Strong Th1-Type Immune Response Deleterious to Protection

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