Malus hupehensis leaves extract attenuates obesity, inflammation, and dyslipidemia by modulating lipid metabolism and oxidative stress in high‐fat diet‐induced obese mice

Abstract

Malus hupehensis leaves (MHL) are used to make traditional Chinese tea. In this study, MHL extract was shown to improve metabolic disorders and inflammatory response in high-fat diet-induced obese mice. MHL extract could reduce body weight, and significantly alleviate liver damage and fat accumulation. MHL extract caused a decrease in the levels of ALT, AST, AKP, TC, TG, LDL-C, and an increase in the level of HDL-C. It also caused a decrease in inflammatory cytokines, including TNF-α, IFN-γ, IL-1β, IL-6, and an increase in the anti-inflammatory cytokine IL-10 and IL-4. MHL extract could upregulate mRNA expression of PPAR-α, LPL, CPT1, CYP7A1, SOD1, SOD2, CAT, GSH1, and GSH-Px and downregulate that of PPAR-γ and C/EBP-α in the liver of obese mice. In conclusion, our work represents the first study demonstrating that MHL extract possesses an anti-obesity effect and alleviates obesity-related symptoms, including dyslipidemia, chronic low-grade inflammatory, and liver damage. Practical applications The research may contribute to the development and application of MHL as functional foods or dietary supplement to fight against obesity.

Structural analysis and anti‐obesity effect of Polygonatum cyrtonema polysaccharide against obesity induced by high‐fat diet in mice

“…FAS also activates CPT1, an essential enzyme during fatty acid β‐oxidation and is associated with diabetes and obesity. The CPT1 gene is a downstream PPAR‐γ target, and PPAR‐γ regulates its expression level to control lipid metabolism and lipid deposition (Lundsgaard et al ., 2018; Wu et al ., 2020). In our study, PCP treatment significantly suppressed the HFD‐induced mice model, which increases after the mRNA expression of PPARγ, C/EBPα and SREBP‐1c, indicating that the PCP effects in reducing body weight were regulated through lipogenesis.…”

Anti‐obesity effect of Auricularia heimuer fruiting body alcohol extraction on obese mice and crucial metabolite pathway analysis by liquid chromatography‐tandem mass spectrometry

“…The mixture was whirled for 1 min and then centrifuged at 15,000 g , at 4°C for 10 min. The collected supernatant was used for liquid chromatography‐tandem mass spectrometry (LC‐MS/MS) analysis (Wu, Guo, et al., 2020; Wu, Sun, et al., 2020; Xie et al., 2020).…”

“…It is a high‐risk factor for a number of severe diseases including diabetes, arteriosclerosis, high blood pressure, circulatory diseases, and respiratory diseases (Kim, Koo, et al., 2020; Kim, Kim, et al., 2020; Terao et al., 2020). Among the various causes of obesity, the imbalance between energy storage and consumption is crucial (Gan et al., 2020; Wu, Guo, et al., 2020; Wu, Sun, et al., 2020). Many epidemiologic studies have reported that excessive nutrition and lack of exercise result in the accumulation of fat and obesity (Yang et al., 2020).…”

“…It not only tastes delicious but also has high medicinal value (Wang et al., 2016). The compounds contained in A. heimuer's fruiting body with biologic activity include flavonoids, polyphenols, and polysaccharides (Huang et al., 2020; Wu, Guo, et al., 2020; Wu, Sun, et al., 2020). A. heimuer has antitumor, cholesterol‐lowering, blood lipid‐lowering, antioxidant, antimicrobial, and anti‐inflammatory activities (Sun et al., 2019, 2020).…”

Preventive Effect of Flavonoid Extract from the Peel of Gonggan (Citrus reticulata Blanco Var. Gonggan) on CCl4-Induced Acute Liver Injury in Mice

“…After 10 μL test solution was injected, the chromatographic peak area was obtained at detection wavelength of 285 nm. 22 The compounds in GPFE were analyzed according the chromatographic peak area of standard substance.…”