The sugarcane aphid, Melanaphis sacchari, is a key pest that affects sorghum in Mexico. During 2014 to 2016, in South of Tamaulipas sites and Bajio region in Guanajuato, the populations of this aphid were infected by different species of Hypocrealean fungi. Based in the morphometric identification and molecular characterization, the species associated with sugarcane aphid in South of Tamaulipas were Lecanicillium longisporum, Beauveria bassiana and Isaria javanica. In this region, the higher infection levels were caused by L. longisporum, mortality range from 30.0% to 50.0%. The presence of I. javanica and B. bassiana represented less than 26.0% and 10.0%, respectively. In Guanajuato, the species found corresponded to L. longisporum and B. bassiana. The infection levels of both species in sugarcane aphid populations in Guanajuato sites were less than 1.00%. The natural occurrence of entomopathogenic fungi on sugarcane aphid populations was associated with climactic factors such as temperature and relative humidity and development of infections was possibly affected by abiotic factors such as crop phenological stage and applications of chemical insecticides realized by farmers for control of this aphid. Further studies on the ecology and physiology of these fungi and trials to determine virulence and persistence in M. sacchari populations are needed. This is the first report on natural presence of L. longisporum, B. bassiana and I. javanica causing disease on Melanaphis sacchari in Guanajuato and Tamaulipas, Mexico. Microbiology J. Zambrano-Gutiérrez et al.
Sampling of Aphid PopulationsWithin each location, five sampling points were established (5 × 5 m at each point). There were four points in the corners and one in the middle of each plot. At each point, one sample of 10 sorghum leaves was randomly selected and the number of fungal infected and healthy aphids was recorded. Due to the difficulty of identifying fungal pathogens in the field, infected aphids were collected in plastic containers with an airtight lid (15 cm wide by 7 cm deep). These containers were labeled and then kept at 10˚C for 72 hours until processing. Growth stage of the crop was recorded on each sampling occasion using the growth stages for sorghum proposed by Vanderlip and Reeves [21]. 40Advances in Microbiology J. Zambrano-Gutiérrez et al.
Weather DataThe local temperature, precipitation, and relative humidity (RH) data before sampling were obtained from the local weather station of the National Institute of Forestry, Agriculture and Livestock (INIFAP) ( Table 4).
Isolation of Entomopathogenic Fungi Infecting the Sugarcane AphidThe EPF Hypocreales were isolated using the rain conidia descending method proposed by Papierok and Hajek [22] for Entomopthoralean fungi with some modifications. The mycosed aphids were adhered individually with a double-sided adhesive tape to the lid of a 60-mm-diameter Petri dish with 10 mL of Papa Dextrose Agar (PDA: BD Bioxon, Becton Dickinson of Mexico S. A. de C. V., Cuautitlán Izcalli, state of Mexico). The d...