The first list of Aetalionidae and Membracidae species for western Acre is presented, including the Alto do Juruá regions of Serra do Divisor National Park and the Campus Floresta of the Federal University of Acre. In total, 94 species of treehoppers were collected, of which Centrotinae (Membracidae) is recorded for the first time for Brazil (Abelus maculatus Schimidt), another 16 species are new Brazilian records, and 69 species (Aetalionidae and Membracidae) are new records for Acre. Data on type locality, geographic distribution and images of all species are presented. The males of Lophyraspis fenestrata Sakakibara & Creão-Duarte and Erechtia sanguinolenta (Fairmaire) are known for the first time. The species diversity was compared to four other treehopper surveys in the Amazon region, with similarity indices of about 43.6% for Colombian Amazon (at least 650 km away), 27.6% for Panguana Biological Research Station, Peru (250 km), 20.2% for Adolpho Ducke Forest Reserve, Brazil (1,600 km) and 8.5% for Villa Carmen Biological Station/Los Amigos Biological Station, Peru (600/700 km). The estimated richness of Serra do Divisor National Park was just over 70%, suggesting that local diversity could be significantly higher than that presented in this study.