<i>MicroRNA‐224</i> is upregulated in HepG2 cells and involved in cellular migration and invasion

Li, Qiong; Wang, Ge; Shan, Jinlu; Yang, Zunhua; Wang, Hongzhong; Jin, Feng; Zhen, Ji-Jun; Chen, Chuan; Zhang, Zhimin; Wen, Xinyu; Luo, Xi-Zhong; Wang, Dong

doi:10.1111/j.1440-1746.2009.05971.x

Cited by 77 publications

(84 citation statements)

References 30 publications

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“…Simultaneously, miR-224 also promotes HCC cell proliferation, although its potential target gene is currently unknown. More recently, miR-224 has been confirmed to be involved in the malignant phenotype of HepG2 cells, and is reported to be a significant factor in the regulation of the migration and invasion of HepG2 cells (21); however, the authors did not propose a potential target gene that may serve as an intermediary between miR-224 and metastasis in HCC cells.…”

Section: Introductionmentioning

confidence: 76%

miR-224 functions as an onco-miRNA in hepatocellular carcinoma cells by activating AKT signaling

Ma¹,

Tao²,

Gao³

et al. 2012

Oncology Letters

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Abstract. microRNAs (miRNAs) are a class of small non-coding RNAs that post-transcriptionally regulate gene expression. Increasing evidence has shown that the deregulation of miRNAs is linked to cancer. The overexpression of miR-224 has been reported in several human cancers. The aim of the present study was to investigate the function of miR-224 in the pathogenetic process of hepatocellular carcinoma (HCC), and the precise mechanism underlying its function. Both gain-of-function and loss-of function assays were conducted through transfection with miR-224 mimics and miR-224 inhibitors in the HepG2 liver carcinoma cell line. The data revealed that miR-224 exerts a significant role in promoting cell proliferation, migration and invasion. Western blot analysis showed that the phosphorylation levels of AKT positively correlated with endogenous levels of miR-224. In addition, results from a dual luciferase reporter assay showed that the expression of the serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A β isoform (PPP2R1B) is inhibited by miR-224; thus, it appears that PPP2R1B is a candidate target of miR-224 in HCC. These data suggest that miR-224 plays a significant role in HCC, possibly through the activation of the AKT signaling pathway by targeting PPP2R1B.

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Section: Introductionmentioning

confidence: 76%

miR-224 functions as an onco-miRNA in hepatocellular carcinoma cells by activating AKT signaling

Ma¹,

Tao²,

Gao³

et al. 2012

Oncology Letters

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show abstract

“…miR‐224 is a well‐known miRNA that plays important roles in the development of multiple tumours 28, 29, 30, 31, 32. For example, Liao et al .…”

Section: Discussionmentioning

confidence: 99%

Retracted: MicroRNA‑224 promotes the sensitivity of osteosarcoma cells to cisplatin by targeting Rac1

Geng

Fang

et al. 2016

J Cellular Molecular Medi

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Osteosarcoma is the most common primary bone tumour in children and adolescents. Accumulating evidence has shown that microRNAs (miRNAs) participate in the development of almost all types of cancer. Here, we investigated the role of miR‐224 in the development and progression of osteosarcoma. We demonstrated that miR‐224 was down‐regulated in osteosarcoma cell lines and tissues. Lower miR‐224 levels were correlated with shorter survivalin osteosarcoma patients. Furthermore, overexpression of miR‐224 suppressed osteosarcoma cell proliferation, migration and invasion and contributed to the increased sensitivity of MG‐63 cells to cisplatin. We identified Rac1 as a direct target gene of miR‐224 in osteosarcoma. Rac1 expression was up‐regulated in the osteosarcoma cell lines and tissues, and there was an inverse correlation between Rac1 and miR‐224 expression in osteosarcoma tissues. Furthermore, rescuing Rac1 expression decreased the sensitivity of miR‐224‐overexpressing MG‐63 cells to cisplatin. We also demonstrated that ectopic expression of Rac1 promoted the proliferation, migration and invasion of miR‐224‐overexpressing MG‐63 cells. These data suggest that miR‐224 plays a tumour suppressor role in the development of osteosarcoma and is related to the sensitivity of osteosarcoma to cisplatin.

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“…Indeed, during hepatocellular carcinogenesis it inhibits apoptosis by blocking apoptosis inhibitor-5 (API-5) . But it also stimulates some targets such as PAK4 and MMP9 increasing migration and tumor progression in HepG2 cells (Li et al, 2010b). Serum levels seem to be higher in patients with hepatocellular carcinoma (Li et al, 2011a).…”

Section: Mir-224mentioning

confidence: 98%

HepatomiRNoma: The proposal of a new network of targets for diagnosis, prognosis and therapy in hepatocellular carcinoma

Bronte

Fanale

et al. 2016

Critical Reviews in Oncology/Hematology

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MicroRNA‐224 is upregulated in HepG2 cells and involved in cellular migration and invasion

Abstract: Overexpression of miR-224 was involved in the malignant phenotype of HepG2 cells, and it may be an important factor in regulating the migration and invasion of HepG2 cells.

Cited by 77 publications

References 30 publications

miR-224 functions as an onco-miRNA in hepatocellular carcinoma cells by activating AKT signaling

miR-224 functions as an onco-miRNA in hepatocellular carcinoma cells by activating AKT signaling

Retracted: MicroRNA‑224 promotes the sensitivity of osteosarcoma cells to cisplatin by targeting Rac1

HepatomiRNoma: The proposal of a new network of targets for diagnosis, prognosis and therapy in hepatocellular carcinoma

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