MIG1‐dependent and MIG1‐independent regulation of GAL gene expression in Saccharomyces cerevisiae: role of Imp2p

Alberti, Adriana; Lodi, Tiziana; Ferrero, Iliana; Donnini, Claudia

doi:10.1002/yea.1025

Cited by 7 publications

(3 citation statements)

References 32 publications

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“…No such consensus could be identified in the promoter sequence of IMA3 and IMA4, which makes possible that not-identified consensus variants of Mig1p binding site allow glucose repression. Alternatively, a MIG1-independent glucose regulation may apply to these genes and explain their modest glucose repression, as was already described for the GAL and MAL systems (41,42). Finally, three of the genes (IMA2, IMA3, and IMA4) harbor an STRE motif for the binding of Msn2/Msn4 proteins (43) together with a TATA box at Ϫ125 before the start codon, whose physiological relevance is discussed below.…”

Section: The Expression Of the Imax Genes Is Regulated By Carbon Sourmentioning

confidence: 91%

Characterization of a New Multigene Family Encoding Isomaltases in the Yeast Saccharomyces cerevisiae, the IMA Family

Teste

François

Parrou

2010

Journal of Biological Chemistry

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It has been known for a long time that the yeast Saccharomyces cerevisiae can assimilate ␣-methylglucopyranoside and isomaltose. We here report the identification of 5 genes (YGR287c, YIL172c, YJL216c, YJL221c and YOL157c), which, similar to the SUCx, MALx, or HXTx multigene families, are located in the subtelomeric regions of different chromosomes. They share high nucleotide sequence identities between themselves (66 -100%) and with the MALx2 genes (63-74%). Comparison of their amino acid sequences underlined a substitution of threonine by valine in region II, one of the four highly conserved regions of the ␣-glucosidase family. This change was previously shown to be sufficient to discriminate ␣-1,4-to ␣-1,6-glucosidase activity in YGR287c (Yamamoto, K., Nakayama, A., Yamamoto, Y., and Tabata, S. (2004) Eur. J. Biochem. 271, 3414 -3420). We showed that each of these five genes encodes a protein with ␣-glucosidase activity on isomaltose, and we therefore renamed these genes IMA1 to IMA5 for IsoMAltase. Our results also illustrated that sequence polymorphisms among this family led to interesting variability of gene expression patterns and of catalytic efficiencies on different substrates, which altogether should account for the absence of functional redundancy for growth on isomaltose. Indeed, deletion studies revealed that IMA1/YGR287c encodes the major isomaltase and that growth on isomaltose required the presence of AGT1, which encodes an ␣-glucoside transporter. Expressions of IMA1 and IMA5/ YJL216c were strongly induced by maltose, isomaltose, and ␣-methylglucopyranoside, in accordance with their regulation by the Malx3p-transcription system. The physiological relevance of this IMAx multigene family in S. cerevisiae is discussed.Gene duplication, one of the main driving forces in genome evolution, generates paralogous genes that can acquire specificities by sequence divergence. These redundant genes are defined as multigene families, the most often located within subtelomere sequences. Very recently, Brown and coworkers demonstrated that the extraordinary instability of these regions supports rapid adaptation to novel niches.

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Section: The Expression Of the Imax Genes Is Regulated By Carbon Sourmentioning

confidence: 91%

Characterization of a New Multigene Family Encoding Isomaltases in the Yeast Saccharomyces cerevisiae, the IMA Family

Teste

François

Parrou

2010

Journal of Biological Chemistry

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“…Gal6p exhibits sequence‐independent binding to nucleic acids and its cytosolic localization suggests a role in the regulation of GAL mRNA levels [105,106]. Imp2p is another new example of a transcriptional factor with a Mig1 and Nrg1p negative repressors‐dependent and Gal6p‐independent positive effect on glucose derepression of GAL genes [107,108].…”

Section: Switching On and Off The Regulonmentioning

confidence: 99%

A comparative analysis of the genetic switch between not-so-distant cousins: versus

Rubio‐Texeira

2005

FEMS Yeast Research

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Despite their close phylogenetic relationship, Kluyveromyces lactis and Saccharomyces cerevisiae have adapted their carbon utilization systems to different environments. Although they share identities in the arrangement, sequence and functionality of their GAL gene set, both yeasts have evolved important differences in the GAL genetic switch in accordance to their relative preference for the utilization of galactose as a carbon source. This review provides a comparative overview of the GAL-specific regulatory network in S. cerevisiae and K. lactis, discusses the latest models proposed to explain the transduction of the galactose signal, and describes some of the particularities that both microorganisms display in their regulatory response to different carbon sources. Emphasis is placed on the potential for improved strategies in biotechnological applications using yeasts.

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“…It has been demonstrated that low glucose concentrations activate SNF1 kinase, which result in the phosphorylation of the transcriptional repressor Mig1, causing its translocation to the cytoplasm and derepression of target genes (Alberti et al, 2003). SNF1 can also directly affect the transcription machinery through the interaction with the Srb/mediator complex of RNA polymerase II and histone phosphorylation (Lo et al, 2001).…”

Section: Sugar Signaling Pathwaysmentioning

confidence: 99%

Signaling pathways in a Citrus EST database

et al. 2007

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Citrus spp. are economically important crops, which in Brazil are grown mainly in the State of São Paulo. Citrus cultures are attacked by several pathogens, causing severe yield losses. In order to better understand this culture, the Millenium Project (IAC Cordeirópolis) was launched in order to sequence Citrus ESTs (expressed sequence tags) from different tissues, including leaf, bark, fruit, root and flower. Plants were submitted to biotic and abiotic stresses and investigated under different development stages (adult vs. juvenile). Several cDNA libraries were constructed and the sequences obtained formed the Citrus ESTs database with almost 200,000 sequences. Searches were performed in the Citrus database to investigate the presence of different signaling pathway components. Several of the genes involved in the signaling of sugar, calcium, cytokinin, plant hormones, inositol phosphate, MAPKinase and COP9 were found in the citrus genome and are discussed in this paper. The results obtained may indicate that similar mechanisms described in other plants, such as Arabidopsis, occur in citrus. Further experimental studies must be conducted in order to understand the different signaling pathways present.

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MIG1‐dependent and MIG1‐independent regulation of GAL gene expression in Saccharomyces cerevisiae: role of Imp2p

Cited by 7 publications

References 32 publications

Characterization of a New Multigene Family Encoding Isomaltases in the Yeast Saccharomyces cerevisiae, the IMA Family

Characterization of a New Multigene Family Encoding Isomaltases in the Yeast Saccharomyces cerevisiae, the IMA Family

A comparative analysis of the genetic switch between not-so-distant cousins: versus

Signaling pathways in a Citrus EST database

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