MiR-532-3pinhibited the methylation ofSOCS2to suppress the progression of PC by targetingDNMT3A

Wang, Kaiqiong; Gong, Dongwei; Qiao, Xin; Zheng, Jiaxuan

doi:10.26508/lsa.202201703

Cited by 3 publications

(2 citation statements)

References 36 publications

(38 reference statements)

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“…MiR-760 проявляла себя как онкосупрессор. Изменения экспрессии микроРНК miR-1976, miR-144-5p, miR-532-3p, miR-98-5p также в большинстве своем связаны со злокачественными новообразованиями, такими как рак яичника [68,69], рак легкого [70][71][72][73], колоректальный рак [74][75][76][77], рак поджелудочной железы [78,79].…”

Section: Discussionunclassified

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Plasma miRNA expression in patients with genetically confirmed multiple endocrine neoplasia type 1 syndrome and its phenocopies

Trukhina,

Mamedova,

Nikitin

et al. 2024

Probl Endokrinol (Mosk)

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BACKGROUND: MEN-1 is a rare autosomal dominant disease caused by mutations in MEN1 gene encoding the menin protein. This syndrome is characterized by the occurrence of parathyroid tumors, gastroenteropancreatic neuroendocrine tumors, pituitary adenomas, as well as other endocrine and non-endocrine tumors. If a patient with the MEN-1 phenotype carry no mutations in the MEN1 gene, the condition considers a phenocopy of syndrome (phMEN1). The possible cause of this changes could be changes in epigenetic regulation, particularly in microRNA expression that might affect menin signaling pathways.AIM: to identify differently expressed circulating miRNAs in plasma in patients with genetically confirmed MEN-1 syndrome, its phenocopies and healthy controls.MATERIALS AND METHODS: single-center, case-control study was conducted. We assessed plasma microRNA expression in patients with genetically confirmed MEN-1 (gMEN1), phMEN1 and healthy controls. Morning plasma samples were collected from fasting patients and stored at –80°C. Total RNA isolation was performed using miRNeasy Mini Kit with QIAcube. The libraries were prepared by the QIAseq miRNA Library Kit following the manufacturer. Circulating miRNA sequencing was done on Illumina NextSeq 500 (Illumina). Subsequent data processing was performed using the DESeq2 bioinformatics algorithm.RESULTS: we enrolled 21 consecutive patients with gMEN1 and 11 patients with phMEN1, along with 12 gender matched controls. Median age of gMEN1 was 38,0 [34,0; 41,0]; in phMEN1 — 59,0 [51,0; 60,0]; control — 59,5 [51,5; 62,5]. The gMEN1 group differed in age (p<0.01) but not gender (р=0.739) or BMI (р=0.116) compared to phMEN1 and controls group, the last two groups did not differ by these parameters (p>0.05). 25 microRNA were differently expressed in groups gMEN1 and phMEN1 (21 upregulated microRNAs, 4 — downregulated). Comparison of samples from the phMEN-1 group and relatively healthy controls revealed 10 differently expressed microRNAs: 5 — upregulated; 5 — downregulated. In the gMEN-1 and control groups, 26 differently expressed microRNAs were found: 24 — upregulated; 2 — downregulated. The miRNAs most differing in expression among the groups were selected for further validation by RT-qPCR (in the groups of gMEN1 vs phMEN1 — miR-3613-5p, miR-335-5p, miR-32-5p, miR-425-3p, miR-25-5p, miR-576-5p, miR-215-5p, miR-30a-3p, miR-141-3p, miR-760, miR-501-3p; gMEN1 vs control — miR-1976, miR-144-5p miR-532-3p, miR-375; as well as in phMEN1 vs control — miR-944, miR-191-5p, miR-98-5p).CONCLUSION: In a pilot study, we detected microRNAs that may be expressed differently between patients with gMEN-1 and phMEN-1. The results need to be validated using different measurement method with larger sample size.

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“…Cr яичников Онкосупрессор [68] Немелкоклеточный Cr легких (↓) [70] Колоректальный Cr (↓) [76] Cr ПЖ (↓) [79] miR-98-5p…”

Section: Discussionunclassified

Plasma miRNA expression in patients with genetically confirmed multiple endocrine neoplasia type 1 syndrome and its phenocopies

Trukhina,

Mamedova,

Nikitin

et al. 2024

Probl Endokrinol (Mosk)

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Screening and molecular mechanism research on bile microRNAs associated with chemotherapy efficacy in perihilar cholangiocarcinoma

Fu,

Du,

Dai

et al. 2024

iScience

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Dysregulated expression of the suppressors of cytokine signaling (SOCS) contributes to the development of prostate cancer

Jafarzadeh,

Zandvakili,

Jafarzadeh

et al. 2024

Pathology - Research and Practice

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MiR-532-3pinhibited the methylation ofSOCS2to suppress the progression of PC by targetingDNMT3A

Cited by 3 publications

References 36 publications

Plasma miRNA expression in patients with genetically confirmed multiple endocrine neoplasia type 1 syndrome and its phenocopies

Plasma miRNA expression in patients with genetically confirmed multiple endocrine neoplasia type 1 syndrome and its phenocopies

Screening and molecular mechanism research on bile microRNAs associated with chemotherapy efficacy in perihilar cholangiocarcinoma

Dysregulated expression of the suppressors of cytokine signaling (SOCS) contributes to the development of prostate cancer

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