<i>MtSUPERMAN</i> plays a key role in compound inflorescence and flower development in <i>Medicago truncatula</i>

Rodas, Ana Lucía; Roque, Edelín; Hamza, Rim; Gómez‐Mena, Concepción; Minguet, Eugenio G.; Wen, Jiangqi; Mysore, Kirankumar S.; Beltrán, José Pío; Cañas, Luis A.

doi:10.1111/tpj.15075

Cited by 22 publications

(32 citation statements)

References 87 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…truncatula orthologue, recently described, restricts the proliferation of floral organs as well, but also regulates I2 meristem activity. mtsup mutant plants produced an increased number of abnormal flowers in their I2s, whose stubs were converted into terminal flowers (Rodas et al ., 2020). Therefore, although it is not its only role, MtSUP , as CaRAX1/2a , restricts I2 meristem activity, suggesting that both genes might cooperate in this function.…”

Section: Discussionmentioning

confidence: 99%

The SINGLE FLOWER (SFL) gene encodes a MYB transcription factor that regulates the number of flowers produced by the inflorescence of chickpea

et al. 2022

View full text Add to dashboard Cite

Summary Legumes usually have compound inflorescences, where flowers/pods develop from secondary inflorescences (I2), formed laterally at the primary inflorescence (I1). Number of flowers per I2, characteristic of each legume species, has important ecological and evolutionary relevance as it determines diversity in inflorescence architecture; moreover, it is also agronomically important for its potential impact on yield. Nevertheless, the genetic network controlling the number of flowers per I2 is virtually unknown. Chickpea (Cicer arietinum) typically produces one flower per I2 but single flower (sfl) mutants produce two (double‐pod phenotype). We isolated the SFL gene by mapping the sfl‐d mutation and identifying and characterising a second mutant allele. We analysed the effect of sfl on chickpea inflorescence ontogeny with scanning electron microscopy and studied the expression of SFL and meristem identity genes by RNA in situ hybridisation. We show that SFL corresponds to CaRAX1/2a, which codes a MYB transcription factor specifically expressed in the I2 meristem. Our findings reveal SFL as a central factor controlling chickpea inflorescence architecture, acting in the I2 meristem to regulate the length of the period for which it remains active, and therefore determining the number of floral meristems that it can produce.

show abstract

Section: Discussionmentioning

confidence: 99%

The SINGLE FLOWER (SFL) gene encodes a MYB transcription factor that regulates the number of flowers produced by the inflorescence of chickpea

et al. 2022

View full text Add to dashboard Cite

show abstract

“…It has been recently described that MtSUPERMAN ( Mtsup ), its Medicago truncatula orthologue, also regulates proliferation of floral organs but in addition regulates I2 meristem activity. Thus, mtsup plants produce an increased number of abnormal flowers in their I2s, which rather than forming a stub, get converted into a terminal flower (Rodas et al, 2020). Although it is not its only function, MtSUP, similarly to CaRAX1/2a, contributes to restrict I2 meristem activity, suggesting that both proteins might cooperate in that function.…”

Section: Discussionmentioning

confidence: 99%

TheSINGLE FLOWER (SFL)gene encodes a MYB transcription factor that regulates the number of flowers produced by the inflorescence of chickpea

Caballo

Berbel

Ortega

et al. 2020

Preprint

View full text Add to dashboard Cite

SUMMARYresearch conducted & rationaleLegume species usually have compound inflorescences, where flowers appear in secondary inflorescences (I2), at lateral positions of the primary inflorescence (I1), in contrast to simple inflorescences, as in Arabidopsis, where flowers are formed in the primary inflorescence stem. The number of flowers per I2, characteristic of each legume species, determines inflorescence diversity, and the number of pods produced, which can affect yield. Gene Regulatory Network that controls the activity of I2 meristems, and therefore the number of flowers per secondary inflorescence is mostly unknown, as well as how specific are factors controlling this trait and whether they share this function in other meristems.methodsChickpea produces one flower per I2 but single flower (sfl) mutants produce two (double-pod phenotype). By mapping the sfl-d mutation and identification and analysis of a second mutant allele we have isolated SFL. We used scanning electron microscopy to study the effect of sfl mutations on inflorescence ontogeny and in situ hybridization to study the expression of SFL and of meristem identity genes in the developing chickpea inflorescence.key resultWe show that the SFL gene corresponds to CaRAX1/2a, encoding a MYB transcription factor. Our results show that CaRAX1/2a / SFL is specifically expressed in the I2 meristem, possibly activated by CaVEGETATIVE1.main conclusion & key points for discussionOur findings reveal that SFL plays a central role in the control of chickpea inflorescence architecture, specifically acting in the I2 meristem to control the time length for which it is active, and therefore determining the number of floral meristems that it can produce.

show abstract

“…CRISPR/Cas9 gene-editing tool has also been used in studies focused on flowering or secondary metabolite production. Rodas et al (2021) edited the M. truncatula SUPERMAN ( MtSUP ) gene with CRISPR/Cas 9 and determined the impairment of MtSUP function with observing defects in floral development and inflorescence architecture in mtsup mutant allel carrying plants. Confalonieri et al (2021) also used the CRISPR/Cas9 gene-editing tool to knock out the two cytochrome P 450 genes ( CYP93E2 and CYP72A61 ) that are responsible for soyasapogenol B production in Medicago spp.…”

Section: Applications In Legumesmentioning

confidence: 99%

Gene-Editing Technologies and Applications in Legumes: Progress, Evolution, and Future Prospects

et al. 2022

View full text Add to dashboard Cite

Legumes are rich in protein and phytochemicals and have provided a healthy diet for human beings for thousands of years. In recognition of the important role they play in human nutrition and agricultural production, the researchers have made great efforts to gain new genetic traits in legumes such as yield, stress tolerance, and nutritional quality. In recent years, the significant increase in genomic resources for legume plants has prepared the groundwork for applying cutting-edge breeding technologies, such as transgenic technologies, genome editing, and genomic selection for crop improvement. In addition to the different genome editing technologies including the CRISPR/Cas9-based genome editing system, this review article discusses the recent advances in plant-specific gene-editing methods, as well as problems and potential benefits associated with the improvement of legume crops with important agronomic properties. The genome editing technologies have been effectively used in different legume plants including model legumes like alfalfa and lotus, as well as crops like soybean, cowpea, and chickpea. We also discussed gene-editing methods used in legumes and the improvements of agronomic traits in model and recalcitrant legumes. Despite the immense opportunities genome editing can offer to the breeding of legumes, governmental regulatory restrictions present a major concern. In this context, the comparison of the regulatory framework of genome editing strategies in the European Union and the United States of America was also discussed. Gene-editing technologies have opened up new possibilities for the improvement of significant agronomic traits in legume breeding.

show abstract

MtSUPERMAN plays a key role in compound inflorescence and flower development in Medicago truncatula

Cited by 22 publications

References 87 publications

The SINGLE FLOWER (SFL) gene encodes a MYB transcription factor that regulates the number of flowers produced by the inflorescence of chickpea

The SINGLE FLOWER (SFL) gene encodes a MYB transcription factor that regulates the number of flowers produced by the inflorescence of chickpea

TheSINGLE FLOWER (SFL)gene encodes a MYB transcription factor that regulates the number of flowers produced by the inflorescence of chickpea

Gene-Editing Technologies and Applications in Legumes: Progress, Evolution, and Future Prospects

Contact Info

Product

Resources

About