<i>mucG, mucH,</i>
            and
            <i>mucI</i>
            Modulate Production of Mutanocyclin and Reutericyclins in Streptococcus mutans B04Sm5

Baker, Jonathon L.; Tang, Xiaoyu; LaBonte, Sandra; Uranga, Carla; Edlund, Anna

doi:10.1128/jb.00042-22

Cited by 6 publications

(4 citation statements)

References 41 publications

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“…To our knowledge, these molecules have remained unknown. Limosilactobacillus reuteri species are well known for other antimicrobials such as reutericyclin and reutericin (Gänzle, 2004), though these are not unique to L. reuteri species (Baker et al., 2022). These antimicrobials are especially active against gram‐positive bacteria, pertaining to the significant reductions in the streptococci in the probiotic‐alone conditions, but curiously the Actinomyces species were not as affected.…”

Section: Discussionmentioning

confidence: 99%

Glycerol strengthens probiotic effect of Limosilactobacillus reuteri in oral biofilms: A synergistic synbiotic approach

Holm

Verspecht

Carvalho

et al. 2022

Molecular Oral Microbiology

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Both in vitro and in vivo studies have shown that the probiotic Limosilactobacillus reuteri can improve oral health. Limosilactobacillus reuteri species are known to produce the antimicrobial “reuterin” from glycerol. In order to further increase its antimicrobial activity, this study evaluated the effect of the combined use of glycerol and Limosilactobacillus reuteri (ATCC PTA 5289) in view of using a synergistic synbiotic over a probiotic. An antagonistic agar growth and a multispecies biofilm model showed that the antimicrobial potential of the probiotic was significantly enhanced against periodontal pathobionts and anaerobic commensals when supplemented with glycerol. Synbiotic biofilms also showed a significant reduction in inflammatory expression of human oral keratinocytes (HOK‐18A), but only when the keratinocytes were preincubated with the probiotic. Probiotic preincubation of keratinocytes or probiotic and synbiotic treatment of biofilms alone was insufficient to significantly reduce inflammatory expression. Overall, this study shows that combining glycerol with the probiotic L. reuteri into a synergistic synbiotic can greatly improve the effectiveness of the latter.

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Section: Discussionmentioning

confidence: 99%

Glycerol strengthens probiotic effect of Limosilactobacillus reuteri in oral biofilms: A synergistic synbiotic approach

Holm

Verspecht

Carvalho

et al. 2022

Molecular Oral Microbiology

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show abstract

“…The oral cavity is a diverse ecological environment that is constantly under a variety of environmental stresses ( 38 ). Therefore, oral bacteria, especially S. mutans , are adept at adaptive horizontal gene transfer making for rich accessory genomes ( 18 , 39 – 42 ). In this study, molecular biology approaches were applied to determine if the presence of multiple S. mutans strains are directly related to changes in measurable caries virulence traits.…”

Section: Discussionmentioning

confidence: 99%

Intraspecies interactions of Streptococcus mutans impact biofilm architecture and virulence determinants in childhood dental caries

Momeni,

Cao,

Xie

et al. 2024

mSphere

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Early childhood dental caries (ECC) is the most common chronic disease among children, especially among low socioeconomic populations. Streptococcus mutans is most frequently associated with initiation of ECC. Although many studies report children with multiple S. mutans strains (i.e., genotypes) have greater odds of developing ECC, studies investigating intraspecies interactions in dental caries are lacking. This study investigates the impact of intraspecies interactions on cariogenic and fitness traits of clinical S. mutans isolates using in vitro and in vivo approaches. Association analysis evaluated if presence of multiple S. mutans genotypes within the first year of colonization was associated with caries. Initially, clinical S. mutans isolates from 10 children were evaluated. S. mutans strains (G09 and G18, most prevalent) isolated from one child were used for subsequent analysis. Biofilm analysis was performed for single and mixed cultures to assess cariogenic traits, including biofilm biomass, intra-polysaccharide, pH, and glucan. Confocal laser scanning microscopy (CLSM) and time-lapse imaging were used to evaluate spatial and temporal biofilm dynamics, respectively. A Drosophila model was used to assess colonization in vivo . Results showed the mean biofilm pH was significantly lower in co-cultured biofilms versus monoculture. Doubling of S. mutans biofilms was observed by CLSM and in vivo colonization in Drosophila for co-cultured S. mutans . Individual strains occupied specific domains in co-culture and G09 contributed most to increased co-culture biofilm thickness and colonization in Drosophila . Biofilm formation and acid production displayed distinct signatures in time-lapsed experiments. This study illuminates that intraspecies interactions of S. mutans significantly impacts biofilm acidity, architecture, and colonization. IMPORTANCE This study sheds light on the complex dynamics of a key contributor to early childhood dental caries (ECC) by exploring intraspecies interactions of different S. mutans strains and their impact on cariogenic traits. Utilizing clinical isolates from children with ECC, the research highlights significant differences in biofilm architecture and acid production in mixed versus single genotype cultures. The findings reveal that co-cultured S. mutans strains exhibit increased cell density and acidity, with individual strains occupying distinct domains. These insights, enhanced by use of time-lapsed confocal laser scanning microscopy and a Drosophila model, offer a deeper understanding of ECC pathogenesis and potential avenues for targeted interventions.

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“…(31) Therefore, oral bacteria, especially S. mutans are particularly adept at adaptive horizontal gene transfer making for rich accessory genomes. (16,(32)(33)(34)(35) In this study molecular biology approaches were applied to determine if the presence of multiple S. mutans are directly related to changes in measurable caries virulence traits.…”

Section: Discussionmentioning

confidence: 99%

Intraspecies interactions ofStreptococcus mutansimpact biofilm architecture and virulence determinants in childhood dental caries

Momeni,

Cao,

Xie

et al. 2023

Preprint

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Early childhood dental caries (ECC) is the most common chronic disease among children with a heavy disease burden among low socioeconomic populations.Streptococcus mutansis most frequently associated with initiation of ECC. Many studies report children with multipleS. mutansstrains (i.e., genotypes) having greater odds of developing ECC, studies investigating intraspecies interactions in dental caries are lacking. In this study, the impact of intraspecies interactions on cariogenic and fitness traits of clinicalS. mutansisolates are investigated usingin-vitroandin-vivoapproaches.Initially clinicalS. mutansisolates of 10 children from a longitudinal epidemiological study were evaluated.S. mutansstrains (G09 and G18, most prevalent) isolated from one child were used for subsequent analysis. Association analysis was used to determine if presence of multipleS. mutansgenotypes within the first-year of colonization was associated with caries. Biofilm analysis was performed for single and mixed cultures to assess cariogenic traits, including biofilm biomass, intra-polysaccharide, pH, and glucan. Confocal Laser Scanning Microscopy (CLSM) and time-lapse imaging were used to evaluate spatial and temporal biofilm dynamics, respectively. ADrosophilamodel was used to assess colonizationin-vivo.Mean biofilm pH was significantly lower in co-cultured biofilms as compared with monoculture biofilms. Doubling ofS. mutans in-vitrobiofilms was observed by CLSM andin-vivocolonization inDrosophilafor co-culturedS. mutans. Individual strains occupied specific domains in co-culture and G09 contributed most to increased co-culture biofilm thickness and colonization inDrosophila. Biofilm formation and acid production displayed distinct signatures in time-lapsed experiments.IMPORTANCEThis study sheds light on the complex dynamics of a key contributor to early childhood dental caries (ECC) by exploring intraspecies interactions of differentS. mutansstrains and their impact on cariogenic traits. Utilizing clinical isolates from children with ECC, the research highlights significant differences in biofilm architecture and acid production in mixed versus single genotype cultures. The findings reveal that co-culturedS. mutansstrains exhibit increased cell density and acidity, with individual strains occupying distinct domains. These insights, enhanced by use of time-lapsed Confocal Laser Scanning Microscopy and a Drosophila model, offer a deeper understanding of ECC pathogenesis and potential avenues for targeted interventions.

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mucG, mucH, and mucI Modulate Production of Mutanocyclin and Reutericyclins in Streptococcus mutans B04Sm5

Cited by 6 publications

References 41 publications

Glycerol strengthens probiotic effect of Limosilactobacillus reuteri in oral biofilms: A synergistic synbiotic approach

Glycerol strengthens probiotic effect of Limosilactobacillus reuteri in oral biofilms: A synergistic synbiotic approach

Intraspecies interactions of Streptococcus mutans impact biofilm architecture and virulence determinants in childhood dental caries

Intraspecies interactions ofStreptococcus mutansimpact biofilm architecture and virulence determinants in childhood dental caries

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