MUN (MERISTEM UNSTRUCTURED), encoding a SPC24 homolog of NDC80 kinetochore complex, affects development through cell division in Arabidopsis thaliana

Shin, Jinwoo; Jeong, Gu-Min; Park, Jong‐Yoon; Kim, Hoyeun; Lee, Ilha

doi:10.1111/tpj.13823

Cited by 24 publications

(36 citation statements)

References 69 publications

(79 reference statements)

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“…When null alleles are not available, alternative methods can be used to determine whether gene function is required for embryo development. One promising approach involves TALEN and/or CRISPR-Cas9-targeted gene inactivation (Moussu et al, 2017;Shin et al, 2018). This strategy should become even more widespread in the future.…”

Section: Reviewmentioning

confidence: 99%

Genome‐wide identification of EMBRYO‐DEFECTIVE (EMB) genes required for growth and development in Arabidopsis

Meinke

2019

New Phytologist

143

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Summary With the emergence of high‐throughput methods in plant biology, the importance of long‐term projects characterized by incremental advances involving multiple laboratories can sometimes be overlooked. Here, I highlight my 40‐year effort to isolate and characterize the most common class of mutants encountered in Arabidopsis (Arabidopsis thaliana): those defective in embryo development. I present an updated dataset of 510 EMBRYO‐DEFECTIVE (EMB) genes identified throughout the Arabidopsis community; include important details on 2200 emb mutants and 241 pigment‐defective embryo (pde) mutants analyzed in my laboratory; provide curated datasets with key features and publication links for each EMB gene identified; revisit past estimates of 500–1000 total EMB genes in Arabidopsis; document 83 double mutant combinations reported to disrupt embryo development; emphasize the importance of following established nomenclature guidelines and acknowledging allele history in research publications; and consider how best to extend community‐based curation and screening efforts to approach saturation for this diverse class of mutants in the future. Continued advances in identifying EMB genes and characterizing their loss‐of‐function mutant alleles are needed to understand genotype‐to‐phenotype relationships in Arabidopsis on a broad scale, and to document the contributions of large numbers of essential genes to plant growth and development.

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Section: Reviewmentioning

confidence: 99%

Genome‐wide identification of EMBRYO‐DEFECTIVE (EMB) genes required for growth and development in Arabidopsis

Meinke

2019

New Phytologist

143

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“…In addition, the green signals of SjNuf2p emerged at the primary constriction of a few chromosomes as an opposite pair ( Supplementary Figure 7). SjNuf2p was proposed to be bound with microtubules at the kelp centromeres, resembling those in yeasts (Osborne et al, 1994;Nabetani et al, 2001;Wigge and Kilmartin, 2001;Asakawa et al, 2005), humans (DeLuca et al, 2002Liu et al, 2007), and Arabidopsis (Ramahi, 2012;Shin et al, 2018). This architecture of the kinetochore microtubule interface was inferred to be a little resistant to the effect of depolymerization by colchicine (Morejohn and Fosket, 1991) from the red immunofluorescence signals of microtubules on the chromosomes after treatment with colchicine ( Figure 5).…”

Section: Discussionmentioning

confidence: 80%

“…A lot of publications uncover the varied centromeric DNA in different organisms, but the kinetochore proteins assembled at the centromeres are evolutionarily conserved (Richards and Dawet, 1998;Jiang et al, 2003;Przewloka and Glover, 2009;Comai et al, 2017). Thus, the attachment of Nuf2p, one component of the Ndc80 complex, with microtubules for the segregation of chromosomes in mitosis (Osborne et al, 1994;Nabetani et al, 2001;DeLuca et al, 2002;Ciferri et al, 2008;Sundin et al, 2011;Shin et al, 2018) drew our attention.…”

Section: Discussionmentioning

confidence: 99%

“…To date, a few reports were focused on Nuf2 mainly in budding yeast S. cerevisiae (Osborne et al, 1994;Wigge and Kilmartin, 2001), fission yeast Schizosaccharomyces pombe (Nabetani et al, 2001;Asakawa et al, 2005), and humans H. sapiens (DeLuca et al, 2002;Liu et al, 2007). Nuf2 is rarely investigated in plants and has only been reported in Arabidopsis thaliana (Ramahi, 2012;Shin et al, 2018). By fusion expression of green fluorescent protein in Arabidopsis, Shin et al (2018) observed that this gene product Nuf2p (GenBank accession No.…”

Section: Introductionmentioning

confidence: 99%

“…Nuf2 is rarely investigated in plants and has only been reported in Arabidopsis thaliana (Ramahi, 2012;Shin et al, 2018). By fusion expression of green fluorescent protein in Arabidopsis, Shin et al (2018) observed that this gene product Nuf2p (GenBank accession No. AEE33765) was co-localized with the Ndc80 complex at the outer kinetochore for the binding to the acidic tails of the αand β-tubulin dimers in microtubules.…”

Section: Introductionmentioning

confidence: 99%

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Immunocytochemical Localization of the Kinetochore Protein Nuf2p on the Gametophyte Chromosomes of a Cultivar of Saccharina (Phaeophyta)

Dong

Liu

et al. 2020

Front. Mar. Sci.

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Nuclear filament-containing protein 2 (Nuf2p), a centromere-associated protein, is an indispensable component of Ndc80 complex, which is important for stable microtubulekinetochore attachment, chromosome pairing, and spindle assembly in mitosis. Based upon contig sequences coding for Nuf2p from a pyrosequencing transcriptome of Saccharina japonica, primers were designed for molecular cloning of this kinetochore protein. The open reading frame (ORF) of this gene SjNuf2 was 1,452 bp long, encoding a putative protein consisting of 483 amino acids. Multi-sequence alignment showed SjNuf2p possessed several conserved domains of Nuf2, Spc7, SPT2, and MIT. To illustrate the location of SjNuf2p using immunocytochemistry, its ORF was inserted into a prokaryotic expression vector pET-28a. Then, the recombinant SjNuf2p (rSjNuf2p) was induced to be expressed in Escherichia coli. The rSjNuf2p was verified by Western blotting with the commercial anti-6 × His-tag antibody and then purified using affinity chromatography. The polyclonal antibody of anti-SjNuf2p was raised against the purified rSjNuf2p. Western blotting with this prepared antibody showed the presence of SjNuf2p in the gametophytes of a Saccharina cultivar. SjNuf2p and α/β-tubulin were subsequently co-localized to the paraffin sections of kelp chromosomes with their corresponding antibodies, thus illustrating that SjNuf2p could reside at the kelp centromeres. This study laid a solid foundation for kelp centromere and karyotype analyses.

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Integrative transcriptomics reveals genotypic impact on sugar beet storability

Madritsch

Bomers

Posekany³

et al. 2020

Plant Mol Biol

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Key message An integrative comparative transcriptomic approach on six sugar beet varieties showing different amount of sucrose loss during storage revealed genotype-specific main driver genes and pathways characterizing storability. Abstract Sugar beet is next to sugar cane one of the most important sugar crops accounting for about 15% of the sucrose produced worldwide. Since its processing is increasingly centralized, storage of beet roots over an extended time has become necessary. Sucrose loss during storage is a major concern for the sugar industry because the accumulation of invert sugar and byproducts severely affect sucrose manufacturing. This loss is mainly due to ongoing respiration, but changes in cell wall composition and pathogen infestation also contribute. While some varieties can cope better during storage, the underlying molecular mechanisms are currently undiscovered. We applied integrative transcriptomics on six varieties exhibiting different levels of sucrose loss during storage. Already prior to storage, well storable varieties were characterized by a higher number of parenchyma cells, a smaller cell area, and a thinner periderm. Supporting these findings, transcriptomics identified changes in genes involved in cell wall modifications. After 13 weeks of storage, over 900 differentially expressed genes were detected between well and badly storable varieties, mainly in the category of defense response but also in carbohydrate metabolism and the phenylpropanoid pathway. These findings were confirmed by gene co-expression network analysis where hub genes were identified as main drivers of invert sugar accumulation and sucrose loss. Our data provide insight into transcriptional changes in sugar beet roots during storage resulting in the characterization of key pathways and hub genes that might be further used as markers to improve pathogen resistance and storage properties.

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MUN (MERISTEM UNSTRUCTURED), encoding a SPC24 homolog of NDC80 kinetochore complex, affects development through cell division in Arabidopsis thaliana

Cited by 24 publications

References 69 publications

Genome‐wide identification of EMBRYO‐DEFECTIVE (EMB) genes required for growth and development in Arabidopsis

Genome‐wide identification of EMBRYO‐DEFECTIVE (EMB) genes required for growth and development in Arabidopsis

Immunocytochemical Localization of the Kinetochore Protein Nuf2p on the Gametophyte Chromosomes of a Cultivar of Saccharina (Phaeophyta)

Integrative transcriptomics reveals genotypic impact on sugar beet storability

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