Musa balbisiana Fruit Rich in Polyphenols Attenuates Isoproterenol-Induced Cardiac Hypertrophy in Rats via Inhibition of Inflammation and Oxidative Stress

Abstract

Musa balbisiana Colla (Family: Musaceae), commonly known as banana and native to India and other parts of Asia, is very rich in nutritional value and has strong antioxidant potential. In the present study, we have developed Musa balbisiana (MB) fruit pulp powder and evaluated its cardioprotective effect in cardiac hypertrophy, which is often associated with inflammation and oxidative stress. An ultra-high-pressure liquid chromatography-mass spectrometer (UPLC-MS/MS) has been used for the detection and systematic characterization of the phenolic compounds present in Musa balbisiana fruit pulp. The cardioprotective effect of MB was evaluated in a rat model of isoproterenol- (ISO-) induced cardiac hypertrophy by subcutaneous administration of isoproterenol (5 mg/kg-1/day-1), delivered through an alzet minipump for 14 days. Oral administration of MB fruit pulp powder (200 mg/kg/day) significantly (p<0.001) decreased heart weight/tail length ratio and cardiac hypertrophy markers like ANP, BNP, β-MHC, and collagen-1 gene expression. MB also attenuated ISO-induced cardiac inflammation and oxidative stress. The in vivo data were further confirmed in vitro in H9c2 cells where the antihypertrophic and anti-inflammatory effect of the aqueous extract of MB was observed in the presence of ISO and lipopolysaccharide (LPS), respectively. This study strongly suggests that supplementation of dried Musa balbisiana fruit powder can be useful for the prevention of cardiac hypertrophy via the inhibition of inflammation and oxidative stress.

Protective Effects Induced by a Hydroalcoholic Allium sativum Extract in Isolated Mouse Heart

“…The inhibitory effects induced by the garlic hydroalcoholic extract on 8-iso-PGF 2α levels could be related to its phenolic acid and flavonoid content, with particular regard to the levels of protocatechuic acid and gallic acid [ 41 , 51 , 52 , 53 , 54 ]. In this context, polyphenol compounds were found to be able to exert cardioprotective effects through the inhibition of oxidative stress and inflammation [ 55 , 56 , 57 , 58 , 59 ]. In particular, it has been demonstrated that protocatechuic acid, one of the main secondary metabolites of garlic, exhibits antioxidant and anti-inflammatory effects [ 60 ].…”

Oxidative stress in cardiac hypertrophy: From molecular mechanisms to novel therapeutic targets

“…In this regard, several naturally occurring compounds have been shown to alleviate the hypertrophic phenotype through suppression of oxidative stress. For instance, the pulp powder extracted from Musa balbisiana (banana) [90], the lowbush blueberry extract (Vaccinium angustifolium) [91], the aqueous extract of the Terminalia arjuna bark [92] and pomegranate juice [93] have been associated with reduction in heart weight, fibrosis, inflammation and oxidative stress. In addition to supressing cardiac hypertrophy, these compounds have been reported to exert beneficial effects across several pathologies suggestive of pleiotropic function.…”

Protective Effects of Caesalpinia sappan Linn. and Its Bioactive Compounds on Cardiovascular Organs

“…It also did not indicate the type of the extract used. An elevation in oxidative stress and inflammation has been reported in isoproterenol-induced myocardial injury ( Kumari et al, 2020 ; Younis et al, 2020 ). Therefore, the extract most likely elicits protective effects through its antioxidant and anti-inflammatory properties, as previously reported ( Tewtrakul et al, 2015 ; Suwan et al, 2018 ).…”