<i>Mycobacterium</i> mimicking metastatic melanoma

Teh, Ru-Wen; Feeney, Kynan; Francis, Roslyn J.; Phillips, Martin; Millward, Michael

doi:10.1111/imj.12209

Cited by 4 publications

(1 citation statement)

References 14 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The traditional detection techniques ( Kromer et al., 2019 ) mostly include histopathology, skin tissue culture, and PCR-bases methods ( Abdalla et al., 2009 ). Histopathologic analyses are nonspecific usually manifesting in the form of granulomatous inflammatory cell infiltrates that are difficult to differentiate from syphilis, granulomatous rosacea, skin tumor ( Teh et al., 2013 ), cutaneous leishmaniasis ( Vanlier et al., 2021 ) and deep dermatophytosis. Thus, histopathologic diagnosis is generally combined with Ziehl-Neelsen staining, particularly for lesions that have a high bacterial load.…”

Section: Discussionmentioning

confidence: 99%

DNA microarray chip assay in new use: early diagnostic value in cutaneous mycobacterial infection

Wang

Gao

et al. 2023

Front. Cell. Infect. Microbiol.

View full text Add to dashboard Cite

IntroductionThe clinical practicability of DNA microarray chip in detecting the presence of mycobacterial species/isolates directly in the skin tissues has not been evaluated, nor the efficacy of DNA microarray chip as a novel diagnostic tool for the early diagnosis of cutaneous mycobacterial infections is known.MethodsThe present study analyzed the incidence of cutaneous mycobacterial infections in Shanghai and explored the efficacy of a novel DNA microarray chip assay for the clinical diagnosis of the disease from skin tissue specimens compared to traditional detection methods. A total of 60 participants fulfilling the defined diagnostic criteria and confirmed positive for cutaneous mycobacterial infections from 2019 to 2021 were enrolled in the study. Subsequent to recording the participants’ medical history and clinical characteristics, the skin tissue specimens were collected for analyses. The specimens underwent histopathological analyses, skin tissue culture, and DNA microarray chip assay.ResultsIncreased incidence of cutaneous mycobacterial infection was detected from 2019 to 2021. The most common infecting pathogen was M. marinum followed by M. abscessus. The sensitivity, specificity and accuracy of the skin tissue culture method were 70%, 100% and 76.62%, respectively, while that of the DNA microarray chip assay were 91.67%, 100% and 93.51%, respectively. The sensitivity and accuracy of the DNA microarray chip assay were significantly higher than those of the skin tissue culture method. The positive likelihood and diagnostic odds ratio were >10 and >1, respectively for both the methods. The negative likelihood ratio was significantly higher (30% vs 8.33%) and the Youden’s index was significantly lower (70.00% vs 91.67%) in the skin culture method compared to that of the DNA microarray chip assay. There was a significant association of false negative results with a history of antibiotic use in the skin tissue culture method.DiscussionGiven the increasing incidence of cutaneous mycobacterial infections, early diagnosis remains a prime clinical focus. The DNA microarray chip assay provides a simple, rapid, high-throughput, and reliable method for the diagnosis of cutaneous mycobacterial infections with potential for clinical application.

show abstract

Section: Discussionmentioning

confidence: 99%

DNA microarray chip assay in new use: early diagnostic value in cutaneous mycobacterial infection

Wang

Gao

et al. 2023

Front. Cell. Infect. Microbiol.

View full text Add to dashboard Cite

show abstract

Lésions pseudotumorales induites par des agents pathogènes. Introduction

Hofman

Tauziède‐Espariat

2019

Annales de Pathologie

View full text Add to dashboard Cite

What, where and why: exploring fluorodeoxyglucose-PET's ability to localise and differentiate infection from cancer

Douglas

Lau

Thursky

et al. 2017

Current Opinion in Infectious Diseases

View full text Add to dashboard Cite

Patients with cancer are vulnerable to infection and fever, and the causes of these are frequently unclear using conventional diagnostic methods leading to high morbidity and mortality, length of stay and costs of care. FDG-PET/CT, with its unique complementary functional and anatomical information as well as its whole-body imaging capability, has demonstrated use in detecting occult infection in immunocompromised patients, including invasive fungal and occult bacterial infections, as well as defining extent of infection. By demonstrating disease resolution following treatment and allowing earlier cessation of therapy, FDG-PET acts as a key tool for antimicrobial and antifungal stewardship. Limitations include at times poor differentiation between infection, malignancy and sterile inflammation, however, exciting new technologies specific to infectious pathogens may help alleviate that issue. Further prospective randomised research is needed to explore these benefits in a nonbiased fashion.

show abstract

Mycobacterium mimicking metastatic melanoma

Cited by 4 publications

References 14 publications

DNA microarray chip assay in new use: early diagnostic value in cutaneous mycobacterial infection

DNA microarray chip assay in new use: early diagnostic value in cutaneous mycobacterial infection

Lésions pseudotumorales induites par des agents pathogènes. Introduction

What, where and why: exploring fluorodeoxyglucose-PET's ability to localise and differentiate infection from cancer

Contact Info

Product

Resources

About