<i>Mycobacterium tuberculosis</i>
            Complex and Mycobacterial Heat Shock Proteins in Lymph Node Tissue from Patients with Pulmonary Sarcoidosis

Dubaniewicz, Anna; Dubaniewicz-Wybieralska, Mirosława; Sternau, Adam; Zwolska, Zofia; Iżycka-Świeszewska, Ewa; Augustynowicz–Kopeć, Ewa; Skokowski, Jarosław; Singh, Mahavir; Zimnoch, L

doi:10.1128/jcm.01433-06

Cited by 86 publications

(72 citation statements)

References 12 publications

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“…This antigen increases expression of costimulatory molecules, suggesting that MTB-hsp70 positivity could lead to a break in immune tolerance and subsequent autoimmune disease (89). Consistent with these positive serology observations, mycobacterial hsp16, hsp65, and hsp70 have been detected in sarcoidal tissue by immunostaining (77).…”

Section: Immunologic and Antigen Isolationsupporting

confidence: 68%

“…Drake and coworkers found MTB rRNA in 48%, MTB rpoB (RNA polymerase) DNA in 24%, and the MTB-specific insertion sequence IS6110 in 0% of 25 patients with sarcoidosis, but none of these sequences was found in 25 control subjects (76). Other authors have also reported low levels or the absence of the MTB IS6110 sequence in sarcoidosis samples (77,78). Whether this finding reflects the differential sensitivity of these tests or the presence of a MTB-related organism that lacks IS6110 is unknown.…”

Section: Nucleic Acid Identificationmentioning

confidence: 94%

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Evidence for Mycobacteria in Sarcoidosis

Brownell

Ramírez‐Valle

Sanchez

et al. 2011

Am J Respir Cell Mol Biol

108

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Section: Immunologic and Antigen Isolationsupporting

confidence: 68%

Section: Nucleic Acid Identificationmentioning

confidence: 94%

Evidence for Mycobacteria in Sarcoidosis

Brownell

Ramírez‐Valle

Sanchez

et al. 2011

Am J Respir Cell Mol Biol

108

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“…Song and colleagues (3) used matrix-assisted laser desorption/ionization time of flight mass spectrometry to demonstrate the presence of mycobacterial catalase-peroxidase (katG) proteins in sarcoidosis granulomas. Mycobacterial heat-shock proteins were also evident in Polish sarcoidosis granulomas (4). Major histocompatibility complex (MHC) Class II alleles are associated with protection or susceptibility in patients with sarcoidosis (5,6).…”

mentioning

confidence: 97%

Development of a Sarcoidosis Murine Lung Granuloma Model UsingMycobacteriumSuperoxide Dismutase A Peptide

Swaisgood¹,

Oswald-Richter²,

Moeller³

et al. 2011

Am J Respir Cell Mol Biol

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Sarcoidosis is characterized by noncaseating granulomas containing CD4 1 T cells with a Th1 immunophenotype. Although the causative antigens remain unknown, independent studies noted molecular and immunologic evidence of mycobacterial virulence factors in sarcoidosis specimens. A major limiting factor in discovering new insights into the pathogenesis of sarcoidosis is the lack of an animal model. Using a distinct superoxide dismutase A peptide (sodA) associated with sarcoidosis granulomas, we developed a pulmonary model of sarcoidosis granulomatous inflammation. Mice were sensitized by a subcutaneous injection of sodA, incorporated in incomplete Freund's adjuvant (IFA). Control subjects consisted of mice with no sensitization (ConNS), sensitized with IFA only (ConIFA), or with Schistosoma mansoni eggs. Fourteen days later, sensitized mice were challenged by tail-vein injection of naked beads, covalently coupled to sodA peptides or to schistosome egg antigens (SEA). Histologic analysis revealed hilar lymphadenopathy and noncaseating granulomas in the lungs of sodA-treated or SEA-treated mice. Flow cytometry of bronchoalveolar lavage (BAL) demonstrated CD4 1 T-cell responses against sodA peptide in the sodA-sensitized mice only. Cytometric bead analysis revealed significant differences in IL-2 and IFN-g secretion in the BAL fluid of sodA-treated mice, compared with mice that received SEA or naked beads (P 5 0.008, Wilcoxon rank sum test). ConNS and ConIFA mice demonstrated no significant formation of granuloma, and no Th1 immunophenotype. The use of microbial peptides distinct for sarcoidosis reveals a histologic and immunologic profile in the murine model that correlates well with those profiles noted in human sarcoidosis, providing the framework to investigate the molecular basis for the progression or resolution of sarcoidosis.

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“…Previous studies have reported humoral responses to mycobacterial antigens among sarcoidosis subjects (4,15) as well as the detection of mycobacterial nucleic acids and proteins in sarcoidosis granulomas (3,5,15). We performed enzyme-linked immunospot (ELISPOT) assays to assess for cellular recognition of two Mycobacterium sp.…”

mentioning

confidence: 99%

Cellular Recognition of Mycobacterium tuberculosis ESAT-6 and KatG Peptides in Systemic Sarcoidosis

et al. 2007

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Sarcoidosis is an enigmatic disease with a pathology similar to that of tuberculosis. We detected Th-1 immune responses to Mycobacterium tuberculosis ESAT-6 and KatG peptides from peripheral blood mononuclear cells from 15/26 sarcoidosis, 1/24 purified-protein-derivative-negative (PPD؊) (P < 0.0001, Fisher's exact test), and 7/8 PPD-positive (PPD؉) subjects (P ‫؍‬ 0.21). This finding provides immunologic links between mycobacteria and systemic sarcoidosis.

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Mycobacterium tuberculosis Complex and Mycobacterial Heat Shock Proteins in Lymph Node Tissue from Patients with Pulmonary Sarcoidosis

Abstract: We demonstrated that not whole Mycobacterium tuberculosis but its particular antigens, hsp70 Mtb , hsp65 Mtb , and hsp16 Mtb , are present in lymph node tissues of patients with sarcoidosis (SA). hsp16 Mtb occurs in the early stage of SA, whereas hsp70 Mtb… Show more

Cited by 86 publications

References 12 publications

Evidence for Mycobacteria in Sarcoidosis

Evidence for Mycobacteria in Sarcoidosis

Development of a Sarcoidosis Murine Lung Granuloma Model UsingMycobacteriumSuperoxide Dismutase A Peptide

Cellular Recognition of Mycobacterium tuberculosis ESAT-6 and KatG Peptides in Systemic Sarcoidosis

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