<i>Mycobacterium ulcerans</i>in Mosquitoes Captured during Outbreak of Buruli Ulcer, Southeastern Australia

Johnson, Paul D R; Azuolas, Joseph; Lavender, Caroline J.; Wishart, Elwyn; Stinear, Timothy P.; Hayman, John; Brown, Lynne; Jenkin, Grant A.; Fyfe, Janet

doi:10.3201/eid1311.061369

Cited by 219 publications

(268 citation statements)

References 35 publications

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“…Within disease-endemic countries, BU has generally been reported from remote rural areas and is characterized by its patchy and focal distribution. [2][3][4][5][6][7][8][9][10] There is a strong epidemiologic association between BU and wetlands, particularly those with slow-moving or stagnant water. 2,3 Indirect evidence, including the recent report of the first cultivation and characterization of M. ulcerans from an aquatic insect (a water strider, Gerridae), supports the hypothesis that M. ulcerans is an environmental pathogen transmitted to humans.…”

Section: Introductionmentioning

confidence: 99%

Persistence of Mycobacterium ulcerans Disease (Buruli Ulcer) in the Historical Focus of Kasongo Territory, the Democratic Republic of Congo

Suykerbuyk¹,

Wambacq²,

Phanzu³

et al. 2009

The American Journal of Tropical Medicine and Hygiene

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Abstract. Fifty years after the last report of Mycobacterium ulcerans infections (Buruli ulcer [BU]) in Kasongo Territory, Maniema Province, Democratic Republic of Congo (DRC), we conducted a small-scale cross-sectional survey to assess if this historical BU focus was still active and if so to explore the disease epidemiology. Seventy-five active and inactive BU cases were identified on clinical grounds of which two of 28 BU active cases were laboratory confirmed. We used a modified BU02 form to reconstruct the local disease dynamics and we believe that the horrific conflict in eastern DRC and exceptional flooding were the most likely causes of the re-emergence of the disease. There is a need in the DRC to decentralize and integrate surveillance and control activities at local level to increase the effectiveness of patient management.

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Section: Introductionmentioning

confidence: 99%

Persistence of Mycobacterium ulcerans Disease (Buruli Ulcer) in the Historical Focus of Kasongo Territory, the Democratic Republic of Congo

Suykerbuyk¹,

Wambacq²,

Phanzu³

et al. 2009

The American Journal of Tropical Medicine and Hygiene

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“…Whereas human-to-human transmission plays a crucial role for the spread of resistant MTBC and M. leprae strains, according to current knowledge, M. ulcerans is acquired from the environment. 1,[28][29][30] With only a few reported cases of infections contracted from humans, the risk of transmitting resistant strains among populations afflicted with BUD may be considered minimal. 31,32 The rate of drug resistance detected in our study among clinical M. ulcerans isolates obtained between 2004 and 2007 was low (0.9%).…”

Section: Discussionmentioning

confidence: 99%

A Genotypic Approach for Detection, Identification, and Characterization of Drug Resistance in Mycobacterium ulcerans in Clinical Samples and Isolates from Ghana

Beißner

Awua-Boateng

Thompson

et al. 2010

The American Society of Tropical Medicine and Hygiene

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Abstract. Standardized antimycobacterial therapy is considered the treatment of choice for Buruli ulcer disease. To assess the prevalence of drug resistance among clinical Mycobacterium ulcerans isolates in Ghana, we conducted a sequence-based approach to detect mutations associated with drug resistance. We subjected clinical samples to direct DNA sequencing of rpoB and rpsL genes and compared culture and whole-genome extracts regarding the efficiency of sequence analysis; 99.1% ( rpoB ) and 100% ( rpsL ) of the patients harbored M. ulcerans wild type. In one isolate (0.9%), a point mutation of the rpoB gene at codon Ser522 leading to an amino acid change was detected. Culture extracts yielded a significantly higher sequencing efficiency than whole-genome extracts. Our data suggest a low level of drug resistance in Ghana. However, mutations associated with drug resistance do occur and require monitoring. Improved techniques are necessary to enhance the efficiency of sequence analysis of whole-genome extracts.

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“…12 It was also reported when salt marsh mosquitoes trapped in multiple locations near the area of a BU outbreak were tested; the presence of M. ulcerans in pools of mosquito DNA with three separate PCR targets, including IS2404, were confirmed. 13 Mucocutaneous leishmaniasis, exclusively observed in South America, is caused by L. braziliensis and transmitted by sandfly bites. Our case of coinfection suggests a possible common vector, although secondary infection of the sandfly bite with contaminated water while bathing cannot be ruled out.…”

Section: Discussionmentioning

confidence: 99%

A Cutaneous Ulcer Resulting from Mycobacterium ulcerans—Leishmania braziliensis Coinfection in South America

Mougin¹,

Avenel-Audran²,

Hasseine³

et al. 2011

The American Society of Tropical Medicine and Hygiene

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Abstract. Buruli ulcer is a tropical skin disease caused by Mycobacterium ulcerans . Its mode of transmission is not yet clearly understood. We report here a cutaneous ulcer in a European traveler in South America resulting from a coinfection detected specifically for Mycobacterium ulcerans and Leishmania braziliensis DNA with real-time polymerase chain reaction. This observation of a unique cutaneous ulcer raises the issue about possible modes of transmission of those two pathogens by the same vector.

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Mycobacterium ulceransin Mosquitoes Captured during Outbreak of Buruli Ulcer, Southeastern Australia

Abstract: Mosquitoes positive for M. ulcerans were linked to outbreaks of Buruli ulcer in humans.

Cited by 219 publications

References 35 publications

Persistence of Mycobacterium ulcerans Disease (Buruli Ulcer) in the Historical Focus of Kasongo Territory, the Democratic Republic of Congo

Persistence of Mycobacterium ulcerans Disease (Buruli Ulcer) in the Historical Focus of Kasongo Territory, the Democratic Republic of Congo

A Genotypic Approach for Detection, Identification, and Characterization of Drug Resistance in Mycobacterium ulcerans in Clinical Samples and Isolates from Ghana

A Cutaneous Ulcer Resulting from Mycobacterium ulcerans—Leishmania braziliensis Coinfection in South America

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