<i>Mycoplasma genitalium</i>presence, resistance and epidemiology in Greenland

Gesink, Dionne; Mulvad, Gert; Montgomery-Andersen, Ruth; Poppel, Upaluk; Montgomery-Andersen, Stephan; Binzer, Aka; Vernich, Lee; Frosst, Gillian; Stenz, Flemming; Rink, Elizabeth; Olsen, Ove Rosing; Koch, Anders; Jensen, Jørgen Skov

doi:10.3402/ijch.v71i0.18203

Cited by 90 publications

(85 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The population used in the present study was selected from Inuulluataarneq (the Greenland Sexual Health Project), which included 314 residents (196 women and 118 men) from Nuuk and Sisimiut, Greenland, between July 2008 and November 2009. Participants were recruited from the general population via the population registry, by direct contact via telephone calls, and by advertising in local media as previously described (14). The initial objective of this study was to determine the epidemiology of sexually transmitted infections (STIs) (14) and BV in Greenland (10).…”

Section: Methodsmentioning

confidence: 99%

“…Participants were recruited from the general population via the population registry, by direct contact via telephone calls, and by advertising in local media as previously described (14). The initial objective of this study was to determine the epidemiology of sexually transmitted infections (STIs) (14) and BV in Greenland (10). Each participant completed an interviewer-administered sexual health survey in either Greenlandic or Danish and provided a self-obtained vaginal smear, a vaginal swab sample collected with a flocked swab in UTM (Copan, Brescia, Italy), and an FVU sample collected in GeneLock transport medium (Sierra Molecular Corp., Sonora, CA) as previously described (14).…”

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Bacterial Vaginosis Diagnosed by Analysis of First-Void-Urine Specimens

et al. 2014

Self Cite

View full text Add to dashboard Cite

e Bacterial vaginosis (BV) is traditionally diagnosed using vaginal samples. The aim of this study was to investigate whether BV can be diagnosed from first-void urine (FVU). Self-collected vaginal smears, vaginal swabs, and FVU were obtained from 176 women. BV was diagnosed by Nugent's criteria. The FVU and vaginal swabs were analyzed by quantitative PCRs (qPCRs) for selected vaginal bacteria (Atopobium vaginae, Prevotella spp., Gardnerella vaginalis, bacterial vaginosis-associated bacterium 2, Eggerthella-like bacterium, "Leptotrichia amnionii," Megasphaera type 1), and all had an area under the receiver operating characteristic (ROC) curve of >85%, suggesting good prediction of BV according to the Nugent score. All seven bacteria in FVU were significantly associated with BV in univariate analysis. An accurate diagnosis of BV from urine was obtained in this population by a combination of qPCRs for Megasphaera type 1 and Prevotella spp. The same two bacteria remained significantly associated with BV in a multivariate model after adjusting for the other five species. There was no statistically significant difference between the sensitivities and specificities of BV diagnosis by molecular methods performed on swabs and FVU samples. A linear regression analysis showed good agreement between bacterial loads from swabs and FVU, but Prevotella spp. could be detected in high numbers in a few FVU samples without being present in swabs. This method will allow diagnosis of BV in studies where only urine has been collected and where detection of BV is considered relevant. Bacterial vaginosis (BV) is an alteration of the normal Lactobacillus sp.-dominated vaginal flora toward a more diverse bacterial flora with overgrowth of facultative and strict anaerobic microorganisms. The BV flora includes anaerobes such as Gardnerella vaginalis, Prevotella spp., Peptostreptococcus spp., Mobiluncus spp., and several uncultured species, e.g., bacterial vaginosis-associated bacteria (BVAB) 1, 2, 3, and TM7, Megasphaera spp., and Eggerthella-like uncultured bacteria (1, 2).The classical and most commonly used algorithms for diagnosing BV are represented by clinical and microscopic classification criteria. The former were established by Amsel et al. (3) and consist of a set of four characteristics, including a homogenous, white vaginal discharge, a fishy odor of the vaginal discharge before or after addition of 10% potassium hydroxide, pH of the vaginal fluid elevated above 4.5, and the presence of clue cells (squamous epithelial cells covered with adherent bacteria). At least three of the four criteria must be present to diagnose BV. Different microscopy scores to diagnose BV have been established, some of them based on Gram-stained vaginal smears (4-6) and others based on wet-smear microscopy (7,8). Among these, the criteria published by Nugent et al. (5) are the most widely used and are considered the gold standard for diagnosing BV.Several studies on the diagnosis of BV using PCR have been published recently (9-13), but all these have...

show abstract

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Bacterial Vaginosis Diagnosed by Analysis of First-Void-Urine Specimens

et al. 2014

Self Cite

View full text Add to dashboard Cite

show abstract

“…Macrolide-resistant M. genitalium isolates have been detected in patients from Australia, Sweden, Norway, and the United States, and mutations in domain V of the 23S rRNA gene have been detected in specimens from Greenland, Japan, France, and an increasing number of other countries (8,9,(12)(13)(14). The resistance mutations consist mainly of adenine-to-guanine mutations in the 23S rRNA gene at position 2058 or 2059 (Escherichia coli numbering) (9,12,15,16).…”

mentioning

confidence: 99%

Direct Detection of Macrolide Resistance in Mycoplasma genitalium Isolates from Clinical Specimens from France by Use of Real-Time PCR and Melting Curve Analysis

et al. 2014

View full text Add to dashboard Cite

“…The second component of Inuulluataarneq involved parent/guardian education regarding sexual health communication with their youth. Prior to the development, design, implementation and evaluation of Inuulluataarneq, we conducted a two-year exploratory study in Greenland (2007Greenland ( -2009, in which Kalaallit youth reported a lack of communication with parents or guardians about topics related to sex, such as STIs (Gesink et al, 2010;Gesink et al, 2012). The results from this study were used to develop Inuulluataarneq's parent/guardian component (Rink et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

“Today we are not good at talking about these things”: A mixed methods study of Inuit parent/guardian-youth sexual health communication in Greenland

Rink¹,

Montgomery-Andersen²,

Anastario³

2014

IJIH

Self Cite

View full text Add to dashboard Cite

Some of the highest rates of sexually transmitted infections (STIs) are reported in the Arctic. For example, the Inuit youth of Greenland have the highest STI rates in the circumpolar region. In this study, we used a mixed method approach to examine parent/guardian-youth communication about STIs and other sexual health topics. The quantitative component of the study involved the parents/guardians completing a brief questionnaire while the qualitative component involved their participation in focus groups. Parents/guardians reported that, although they found little difficulty communicating with youth in general, this was not the case for sexual topics. Similarly, parents/guardians reported a lack of communication about topics such as masturbation, why people engage in sex, and what it feels like to have sex. In general parents/guardians stated that it is uncomfortable for them to talk with their youth about sexual matters. At the same time, they are concerned about the high rates of unwanted pregnancy in their communities, and they would like to see more collaborative partnerships aimed at increasing sexual health education for youth. Future STI prevention efforts in Greenland would benefit from involving Greenlandic youth and their families in the design of community-based sexual health education programs that increase communication skills in families about topics related to sex. Community based efforts that involve families and empower them to educate their youth around STI risk-prevention behaviours is a necessary area of future development in Greenland.

show abstract

Mycoplasma genitaliumpresence, resistance and epidemiology in Greenland

Cited by 90 publications

References 34 publications

Bacterial Vaginosis Diagnosed by Analysis of First-Void-Urine Specimens

Bacterial Vaginosis Diagnosed by Analysis of First-Void-Urine Specimens

Direct Detection of Macrolide Resistance in Mycoplasma genitalium Isolates from Clinical Specimens from France by Use of Real-Time PCR and Melting Curve Analysis

“Today we are not good at talking about these things”: A mixed methods study of Inuit parent/guardian-youth sexual health communication in Greenland

Contact Info

Product

Resources

About