Mycosphaerella suberosaandM. intermediasp. nov. onEucalyptusin New Zealand

Dick, M. A.; Dobbie, K.

doi:10.1080/0028825x.2001.9512737

Cited by 20 publications

(13 citation statements)

References 13 publications

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“…The high degree of interspecific ITS sequence similarity and lack of alternative well-characterized genomic loci preclude differentiation of each currently recognized species in these groups. Additional studies based on collections from many hosts and geographic regions are needed to confirm that these species are distinct taxa and not merely subpopulations of a single species (22). The ecological niche occupied or pathogenicity factors are secondary taxonomic characters for Mycosphaerella spp.…”

Section: Discussionmentioning

confidence: 99%

“…The most damaging are reported to be M. nubilosa and M. cryptica (10)(11)(12)14,25,(59)(60)(61)(62), which cause the most severe leaf disease, resulting in significant crown damage of E. globulus, E. nitens, and their hybrids (30,39), the most widely grown temperate plantation species. Crown damage in E. globulus in Australia has been reported to range from 10% leaf necrosis to complete defoliation and tree death in locations strongly conducive to disease development (22,23,50,52). MLD has restricted the use of commercially desirable eucalypt species in Australia, New Zealand, and South Africa (28).…”

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confidence: 99%

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Development of Nested Polymerase Chain Reaction Detection ofMycosphaerellaspp. and Its Application to the Study of Leaf Disease inEucalyptusPlantations

Glen¹,

Smith²,

Langrell³

et al. 2007

Phytopathology®

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Glen, M., Smith, A. H., Langrell, S. R. H., and Mohammed, C. L. 2007. Development of nested polymerase chain reaction detection of Mycosphaerella spp. and its application to the study of leaf disease in Eucalyptus plantations. Phytopathology 97:132-144.Mycosphaerella leaf disease (MLD) is a serious disease of two of the major eucalypt species grown in temperate regions worldwide, Eucalyptus globulus and E. nitens. More than 30 species of Mycosphaerella have been reported on eucalypts worldwide. Accurate, rapid, and early discrimination of Mycosphaerella spp. causing crown damage to E. globulus and E. nitens will assist the development of sustainable management strategies. This study describes the development, and incorporation in a nested polymerase chain reaction (PCR) approach, of specific primers for the detection and identification of Mycosphaerella spp. commonly reported from leaf lesions of E. globulus and E. nitens in Australia. Primer design was assisted by sequence alignment and phylogenetic analysis of 165 nonredundant sequences from the nuclear ribosomal DNA internal transcribed spacer regions of Mycosphaerella and related species. Phylogenetic analysis revealed very high sequence similarity for two taxon groups, Mycosphaerella grandis and M. parva, and M. vespa, M. ambiphylla, and M. molleriana, and primers were designed to differentiate each of the two groups. Three other species, M. cryptica, M. nubilosa, and M. tasmaniensis, were distinct and distinguished by species-specific primers. In double-blind trials, the detection test accurately and rapidly identified Mycosphaerella spp. in cultures and discriminated against other pathogens that co-occur in or on Eucalyptus leaves, thereby verifying its reliability. The detection test has an internal amplification control in the first-round PCR with fungal-specific primers to raise confidence in test results, particularly to highlight negative results due to PCR inhibition. When applied to DNA extracted from leaf or stem samples either as multiple or single lesions, it detected and identified up to five Mycosphaerella spp. or taxon groups in both positively identified and in young (putative) MLD lesions. The samples were 20 mm 2 or larger in surface area and were collected while undertaking disease rating assessments in an experimental investigation of Eucalyptus plantations and regrowth forest. Using nested PCR detection, Mycosphaerella spp. were positively identified in 2 days, 1 to 5 months earlier than by classical methods, demonstrating the potential application of this detection test to the early discrimination of MLD components in ecological, epidemiological, and genetic investigations.

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Section: Discussionmentioning

confidence: 99%

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confidence: 99%

Development of Nested Polymerase Chain Reaction Detection ofMycosphaerellaspp. and Its Application to the Study of Leaf Disease inEucalyptusPlantations

Glen¹,

Smith²,

Langrell³

et al. 2007

Phytopathology®

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“…This includes the 27 species recognised in Crous (1998) and the newly described M. vespa and M. intermedia (Dick & Dobbie 2001).…”

Section: Discussionmentioning

confidence: 99%

Mycosphaerella species associated with Eucalyptus in south-western Australia: new species, new records and a key

Maxwell

Dell

Neumeister-Kemp

et al. 2003

Mycological Research

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“…Leaf spots can be brown (T. ambiphylla) (Maxwell et al 2003), dark brown and corky with a yellow-red margin (T. suberosa) (Dick and Dobbie 2001), yellow to brown (T. nubilosa) (Crous et al 1989a;Crous 1998), grey to pale brown (M. tasmaniensis, P. eucalyptorum) (Crous et al 1989a;1998), pale brown to red-brown [T. molleriana (as M.…”

Section: Comparison Of Mycosphaerella and Teratosphaeria Diseasesmentioning

confidence: 99%

Mycosphaerella and Teratosphaeria diseases of Eucalyptus; easily confused and with serious consequences

et al. 2011

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The Mycosphaerella complex accommodates thousands of taxa. Many of these species are economically important plant pathogens, notably on native and commercially propagated Eucalyptus species where they cause a wide range of disease symptoms including leaf spot, leaf blotch, shoot blight and stem cankers. Some of these diseases represent major impediments to sustainable Eucalyptus forestry in several countries where infection by Mycosphaerella and Teratosphaeria species can result in reduction of wood volume and in severe cases tree death.Extensive research has been conducted on these disease complexes over the past 40 years. The incorporation of DNA-based molecular techniques has made it possible to define and to better understand the differences between the Mycosphaerella and Teratosphaeria species occurring on Eucalyptus. These studies have also enabled refinement of anamorph and teleomorph generic concepts for the genera and thus facilitated the more accurate identification of species. They have also promoted a more lucid understanding of the biology, life cycles, population biology and epidemiology of the most important pathogens in the group.

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Mycosphaerella suberosaandM. intermediasp. nov. onEucalyptusin New Zealand

Cited by 20 publications

References 13 publications

Development of Nested Polymerase Chain Reaction Detection ofMycosphaerellaspp. and Its Application to the Study of Leaf Disease inEucalyptusPlantations

Development of Nested Polymerase Chain Reaction Detection ofMycosphaerellaspp. and Its Application to the Study of Leaf Disease inEucalyptusPlantations

Mycosphaerella species associated with Eucalyptus in south-western Australia: new species, new records and a key

Mycosphaerella and Teratosphaeria diseases of Eucalyptus; easily confused and with serious consequences

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