<i>N</i>-acetylcysteine infusion alters blood redox status but not time to fatigue during intense exercise in humans

Medved, Ivan; Brown, Malcolm J.; Bjorksten, Andrew R.; Leppik, James A; Sostaric, Simon; McKenna, Michael J.

doi:10.1152/japplphysiol.00884.2002

Cited by 100 publications

(154 citation statements)

References 52 publications

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“…GSH, GSSG, Cys, CySS, iodoacetic acid (IAA), [1,[2][3][4][5][6][7][8][9][10][11][12][13] C]-IAA, acetonitrile, HPLC grade water, formic acid (98%) and ammonium bicarbonate were purchased from Sigma Chemical (St. Louis, MO). IAA was recrystallized twice in petroleum ether.…”

Section: Chemicalsmentioning

confidence: 99%

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A rapid LC-FTMS method for the analysis of cysteine, cystine and cysteine/cystine steady-state redox potential in human plasma

Johnson

Strobel

Reed

et al. 2008

Clinica Chimica Acta

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Background-The steady-state redox potential of the cysteine/cystine couple in human plasma provides a measure of oxidative stress, yet available assays are limited by either specificity or speed of assay.Method-The present study evaluated the use of LC-FTMS for identification based on accurate mass combined with quantification by stable isotopic dilution to rapidly determine cysteine and cystine concentration and cysteine/cystine steady-state redox potential in human plasma.Results-A simple extraction procedure followed by a rapid LC separation eluted cysteine in 4 min and cystine in 1.5 min with simultaneous measurement of glutathione (GSH) and glutathione disulfide (GSSG). A study of five young (mean age = 25.7) subjects and 5 older (mean age = 67.8 y) subjects showed an increased oxidation with age.Conclusions-Analysis by LC-FTMS is suitable for high-throughput analysis of plasma cysteine, cystine and cysteine/cystine steady-state redox potential as clinical measures of oxidative stress.

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Section: Chemicalsmentioning

confidence: 99%

“…The predominant low molecular weight thiol/disulfide system in plasma consists of cysteine (Cys) and cystine (CySS). The steady-state redox potential of this couple has been previously used to measure oxidative stress [1,[6][7][8].…”

Section: Introductionmentioning

confidence: 99%

A rapid LC-FTMS method for the analysis of cysteine, cystine and cysteine/cystine steady-state redox potential in human plasma

Johnson

Strobel

Reed

et al. 2008

Clinica Chimica Acta

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“…In study group, there was a statistically very significant rise (p=0.001) in GSH levels in the postoperative period, implying the role of NAC as a GSH precursor. 10,11 Thus in the postoperative period, there was a significant rise in GSH levels in study group, in contrast to the significant decline in control group.…”

Section: Discussionmentioning

confidence: 77%

“…Medved et al, 11 in their study found that NAC infusion did not induce any severe adverse reactions. After a single intravenous dose of NAC, plasma concentration declined in a poly-exponential decay manner with mean terminal half-life T1/2 of 5.6 hours.…”

Section: Discussionmentioning

confidence: 83%

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Effect of N - Acetylcysteine on Oxidative Stress in Patients Undergoing Off Pump Coronary Artery Bypass Grafting

Jalakandan¹,

Bhaskaran²,

Hiremath³

2015

jemds

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OBJECTIVE: Increasingly used Off Pump Coronary Artery Bypass Grafting (OPCABG) has significantly reduced the oxidative stress and decreased the inflammatory response associated with the use of Cardiopulmonary by Pass (CPB). However, OPCABG is associated with significant oxidative stress and its associated complications. This present study is a prospective, randomized, double blind investigating the effects of N-acetylcysteine (NAC), a potent anti-oxidant on oxidative stress in patients undergoing OPCABG. METHODS: Fifty patients undergoing elective OPCABG were randomized into two groups. Group A (n=25), the control group received 200ml of Normal saline immediately following induction, whereas Group B (n=25), the study group received 150mg/kg of NAC in 200ml of Normal saline at the corresponding time. At the end of surgery, all the patients were shifted to intensive care unit (ICU) and were extubated at the earliest possible time. Malondialdehyde (MDA), a marker of free radical injury and Glutathione (GSH) Levels were assayed from the 2 blood samples obtained (First sample immediately following induction and the second immediately after shifting to ICU). RESULTS: Demographic profile, pre-incision clinical and biochemical values were comparable in both the groups. At the end of surgery, MDA levels were significantly raised in control group (p<0.001) whereas its levels were maintained in study group (p<0.569). GSH levels were significantly decreased in control group (p<0.001) whereas its levels were significantly increased in study group (p<0.001). CONCLUSION: These results revealed that OPCABG was associated with significant oxidative response and the administration of N-Acetylcysteine attenuates this stress response by replenishing the Glutathione stores.

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Reactive Oxygen Species: Impact on Skeletal Muscle

Powers

Kavazis

et al. 2011

Comprehensive Physiology

392

384

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It is well established that contracting muscles produce both reactive oxygen and nitrogen species. Although the sources of oxidant production during exercise continue to be debated, growing evidence suggests that mitochondria are not the dominant source. Regardless of the sources of oxidants in contracting muscles, intense and prolonged exercise can result in oxidative damage to both proteins and lipids in the contracting myocytes. Further, oxidants regulate numerous cell signaling pathways and modulate the expression of many genes. This oxidant-mediated change in gene expression involves changes at transcriptional, mRNA stability, and signal transduction levels. Furthermore, numerous products associated with oxidant-modulated genes have been identified and include antioxidant enzymes, stress proteins, and mitochondrial electron transport proteins. Interestingly, low and physiological levels of reactive oxygen species are required for normal force production in skeletal muscle, but high levels of reactive oxygen species result in contractile dysfunction and fatigue. Ongoing research continues to explore the redox-sensitive targets in muscle that are responsible for both redox-regulation of muscle adaptation and oxidant-mediated muscle fatigue.

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N-acetylcysteine infusion alters blood redox status but not time to fatigue during intense exercise in humans

Cited by 100 publications

References 52 publications

A rapid LC-FTMS method for the analysis of cysteine, cystine and cysteine/cystine steady-state redox potential in human plasma

A rapid LC-FTMS method for the analysis of cysteine, cystine and cysteine/cystine steady-state redox potential in human plasma

Effect of N - Acetylcysteine on Oxidative Stress in Patients Undergoing Off Pump Coronary Artery Bypass Grafting

Reactive Oxygen Species: Impact on Skeletal Muscle

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