<i>N</i>‐Glycosylation is essential for the secretion of extracellular superoxide dismutase

Ota, Fumi; Kizuka, Yasuhiko; Kitazume, Shinobu; Adachi, Tetsuo; Taniguchi, Naoyuki

doi:10.1002/1873-3468.12378

Cited by 25 publications

(16 citation statements)

References 45 publications

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“…N ‐Glycosylation of the OST subunit ribophorin (DEIGNISTSNVR) as well as calreticulin (EDELTHVYTLIISANNTFK) and a vacuolar sorting receptor (NITIPAAFIK) is in line with reports from other organism (Denning et al ., ; Sanjay et al ., ; Shen et al ., ). Superoxide dismutase (NQAGGDFNHIIYFSTLKPNGSQPSER) and vanadium‐dependent haloperoxidase (DFNITNQISAR) are both connected to hydrogen peroxide with N ‐glycosylation of the former being described as essential for its secretion (Ota et al ., ). Mannan endo‐1,4‐beta‐mannosidases (WFNQTYDQLR) catalyze the hydrolysis of mannan, a key polymer in hemicelluloses, and were shown to be N ‐glycosylated when expressed in Pichia pastoris (Do et al ., ).…”

Section: Resultsmentioning

confidence: 97%

Identification of methylated GnTI‐dependent N‐glycans in Botryococcus brauni

et al. 2017

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In contrast to mammals and vascular plants, microalgae show a high diversity in the N-glycan structures of complex N-glycoproteins. Although homologues for β1,2-N-acetylglucosaminyltransferase I (GnTI), a key enzyme in the formation of complex N-glycans, have been identified in several algal species, GnTI-dependent N-glycans have not been detected so far. We have performed an N-glycoproteomic analysis of the hydrocarbon oils accumulating green microalgae Botryococcus braunii. Thereby, the analysis of intact N-glycopeptides allowed the determination of N-glycan compositions. Furthermore, insights into the role of N-glycosylation in B. braunii were gained from functional annotation of the identified N-glycoproteins. In total, 517 unique N-glycosylated peptides have been identified, including intact N-glycopeptides that harbored N-acetylhexosamine (HexNAc) at the nonreducing end. Surprisingly, these GnTI-dependent N-glycans were also found to be modified with (di)methylated hexose. The identification of GnTI-dependent N-glycans in combination with N-glycan methylation in B. braunii revealed an uncommon type of N-glycan processing in this microalgae.

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Section: Resultsmentioning

confidence: 97%

Identification of methylated GnTI‐dependent N‐glycans in Botryococcus brauni

et al. 2017

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“…Changes in the glycosylation pattern of Apa lead to a reduced stimulatory T-lymphocyte response, exhibiting the biological role of the glycan moiety 50 . Glycosylation is also required for proper localization of superoxide dismutases (SodB) 51 . The immunogenic glycoproteins MPT64 and Apa are virulence factors involved in Mtb infection of human cells and is a promising candidate for a subunit-based anti-TB vaccine 12,52 .…”

Section: Discussionmentioning

confidence: 99%

Ample glycosylation in membrane and cell envelope proteins may explain the phenotypic diversity and virulence in the Mycobacterium tuberculosis complex

Birhanu

Yimer

Kalayou

et al. 2019

Sci Rep

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Multiple regulatory mechanisms including post-translational modifications (PTMs) confer complexity to the simpler genomes and proteomes of Mycobacterium tuberculosis (Mtb). PTMs such as glycosylation play a significant role in Mtb adaptive processes. The glycoproteomic patterns of clinical isolates of the Mycobacterium tuberculosis complex (MTBC) representing the lineages 3, 4, 5 and 7 were characterized by mass spectrometry. A total of 2944 glycosylation events were discovered in 1325 proteins. This data set represents the highest number of glycosylated proteins identified in Mtb to date. O-glycosylation constituted 83% of the events identified, while 17% of the sites were N-glycosylated. This is the first report on N-linked protein glycosylation in Mtb and in Gram-positive bacteria. Collectively, the bulk of Mtb glycoproteins are involved in cell envelope biosynthesis, fatty acid and lipid metabolism, two-component systems, and pathogen-host interaction that are either surface exposed or located in the cell wall. Quantitative glycoproteomic analysis revealed that 101 sites on 67 proteins involved in Mtb fitness and survival were differentially glycosylated between the four lineages, among which 64% were cell envelope and membrane proteins. The differential glycosylation pattern may contribute to phenotypic variabilities across Mtb lineages. The study identified several clinically important membrane-associated glycolipoproteins that are relevant for diagnostics as well as for drug and vaccine discovery.

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“…Immature EcSOD protein has an N-terminus with targeting signal that instructs its secretion to the extracellular space. Once this targeting signal is cleaved, the mature form gains enhanced protein stability due to a glycosylation of asparagine at position 89 (80).…”

Section: Introductionmentioning

confidence: 99%

Extracellular superoxide dismutase, a molecular transducer of health benefits of exercise

2020

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Extracellular superoxide dismutase (EcSOD) is the only extracellular scavenger of superoxide anion (O 2 .-) with unique binding capacity to cell surface and extracellular matrix through its heparin-binding domain. Enhanced EcSOD activity prevents oxidative stress and damage, which are fundamental in a variety of disease pathologies. In this review we will discuss the findings in humans and animal studies supporting the benefits of EcSOD induced by exercise training in reducing oxidative stress in various tissues. In particularly, we will highlight the importance of skeletal muscle EcSOD, which is induced by endurance exercise and redistributed through the circulation to the peripheral tissues, as a molecular transducer of exercise training to confer protection against oxidative stress and damage in various disease conditions.

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N‐Glycosylation is essential for the secretion of extracellular superoxide dismutase

Cited by 25 publications

References 45 publications

Identification of methylated GnTI‐dependent N‐glycans in Botryococcus brauni

Identification of methylated GnTI‐dependent N‐glycans in Botryococcus brauni

Ample glycosylation in membrane and cell envelope proteins may explain the phenotypic diversity and virulence in the Mycobacterium tuberculosis complex

Extracellular superoxide dismutase, a molecular transducer of health benefits of exercise

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