<i>Narrow Leaf21</i>, encoding ribosomal protein RPS3A, controls leaf development in rice

Uzair, Muhammad; Long, Haixin; Zafar, Syed Adeel; Patil, S. B.; Chun, Yung; Lü, Li; Fang, Jingjing; Zhao, Jinfeng; Peng, Lixiang; Yuan, Shu; Li, Xueyong

doi:10.1093/plphys/kiab075

Cited by 43 publications

(44 citation statements)

References 78 publications

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“…To standardize the expression data, the wheat gene RLI (RNase L inhibitor-like protein) was used as an internal control [38]. The 2-ΔΔCt technique was used to assess gene expression [39,40]. Three biological replicates were used for each gene.…”

Section: Quantitative Rt-pcr Assaymentioning

confidence: 99%

Genome-wide investigation and functional analysis of RNA editing sites in wheat

Rasool

Ishtiaq²,

Uzair³

et al. 2022

PLoS ONE

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Wheat is an important cereal and half of the world population consumed it. Wheat faces environmental stresses and different techniques (CRISPR, gene silencing, GWAS, etc.) were used to enhance its production but RNA editing (RESs) is not fully explored in wheat. RNA editing has a special role in controlling environmental stresses. The genome-wide identification and functional characterization of RESs in different types of wheat genotypes was done. We employed six wheat genotypes by RNA-seq analyses to achieve RESs. The findings revealed that RNA editing events occurred on all chromosomes equally. RNA editing sites were distributed randomly and 10–12 types of RESs were detected in wheat genotypes. Higher number of RESs were detected in drought-tolerant genotypes. A-to-I RNA editing (2952, 2977, 1916, 2576, 3422, and 3459) sites were also identified in six wheat genotypes. Most of the genes were found to be engaged in molecular processes after a Gene Ontology analysis. PPR (pentatricopeptide repeat), OZ1 (organelle zinc-finger), and MORF/RIP gene expression levels in wheat were also examined. Normal growth conditions diverge gene expression of these three different gene families, implying that normal growth conditions for various genotypes can modify RNA editing events and have an impact on gene expression levels. While the expression of PPR genes was not change. We used Variant Effect Predictor (VEP) to annotate RNA editing sites, and Local White had the highest RESs in the CDS region of the protein. These findings will be useful for prediction of RESs in other crops and will be helpful in drought tolerance development in wheat.

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Section: Quantitative Rt-pcr Assaymentioning

confidence: 99%

Genome-wide investigation and functional analysis of RNA editing sites in wheat

Rasool

Ishtiaq²,

Uzair³

et al. 2022

PLoS ONE

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“…However, annual yield gain in crop productivity is prolonged to meet the future demands for plant-based products for the projected global population in 2050 ( Priyamvada et al, 2011 , Tilman et al, 2011 ). Globally, different kinds of stresses are the main threats for production of crops ( Hussain et al, 2011 , Iqbal et al, 2018 , Naveed et al, 2020 , Uzair et al, 2021 ). Plants are immobile, for their growth and for survival they need nutrients and water from the soil.…”

Section: Introductionmentioning

confidence: 99%

The characterization of wheat genotypes for salinity tolerance using morpho-physiological indices under hydroponic conditions

Uzair

Ali

Fiaz

et al. 2022

Saudi Journal of Biological Sciences

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“…Hence, for further RT-PCR experiment reference gene TaActin was used to normalize the relative expression data. Relative expression values were evaluated by using the 2ΔΔCT method [ 37 ].…”

Section: Methodsmentioning

confidence: 99%

Role of Wheat Phosphorus Starvation Tolerance 1 Genes in Phosphorus Acquisition and Root Architecture

Abbas¹,

Naeem²,

Rubab

et al. 2022

Genes

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The wheat plant requires elevated phosphorus levels for its normal growth and yield, but continuously depleting non-renewable phosphorus reserves in the soil is one of the biggest challenges in agricultural production worldwide. The Phosphorus Starvation Tolerance 1 (PSTOL1) gene has been reported to play a key role in efficient P uptake, deeper rooting, and high yield in rice. However, the function of the PSTOL1 gene in wheat is still unclear. In this study, a total of 22 PSTOL1 orthologs were identified in the wheat genome, and found that wheat PSTOL1 orthologs are unevenly distributed on chromosomes, and these genes were under strong purifying selection. Under different phosphorus regimes, wheat PSTOL1 genes showed differential expression patterns in different tissues. These results strengthen the classification of Pakistan-13 as a P-efficient cultivar and Shafaq-06 as a P-inefficient cultivar. Phenotypic characterization demonstrated that Pakistan-13 wheat cultivar has significantly increased P uptake, root length, root volume, and root surface area compared to Shafaq-06. Some wheat PSTOL1 orthologs are co-localized with phosphorus starvation’s related quantitative trait loci (QTLs), suggesting their potential role in phosphorus use efficiency. Altogether, these results highlight the role of the wheat PSTOL1 genes in wheat P uptake, root architecture, and efficient plant growth. This comprehensive study will be helpful for devising sustainable strategies for wheat crop production and adaptation to phosphorus insufficiency.

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Narrow Leaf21, encoding ribosomal protein RPS3A, controls leaf development in rice

Cited by 43 publications

References 78 publications

Genome-wide investigation and functional analysis of RNA editing sites in wheat

Genome-wide investigation and functional analysis of RNA editing sites in wheat

The characterization of wheat genotypes for salinity tolerance using morpho-physiological indices under hydroponic conditions

Role of Wheat Phosphorus Starvation Tolerance 1 Genes in Phosphorus Acquisition and Root Architecture

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