<i>Neisseria gonorrhoeae</i>
            II. Colonial Variation and Pathogenicity During 35 Months In Vitro

Kellogg, Douglas S.; Cohen, Irun R.; Norins, Leslie C.; Schroeter, Arnold L.; Reising, Gilbert

doi:10.1128/jb.96.3.596-605.1968

Cited by 388 publications

(204 citation statements)

References 12 publications

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“…To provide a view of differentially expressed genes, labelled cDNA from cells infected with piliated or non-piliated N. gonorrhoeae or with N. meningitidis was used to probe microarrays comprising 375 genes belonging to the following families: adhesion molecules, angiogenic factors, cell-surface proteins, chemokines and their receptors, cytokines and their receptors, binding proteins, epidermal and fibroblast growth factors, ephrins and ephrin receptors, integrins, interleukins and their receptors, neurotrophic factors, genes involved in nitric oxide metabolism, proteases, orphan receptors and members of the transforming growth factor-b (TGF-b) and TNF-a superfamilies. Pili are essential for adherence of N. gonorrhoeae to target cells and only piliated gonococci are capable of producing gonorrhoea in human male volunteers (Kellogg et al, 1968;Swanson et al, 1987). Non-piliated gonococci adhere at low levels to epithelial cell lines and are do not cause inflammatory responses in the urogenital tracts of human male volunteers (Cohen and Cannon, 1999).…”

Section: Discussionsupporting

confidence: 79%

“…Neisseria meningitidis serogroup C strain FAM20 has been described previously (Dyer et al, 1987). Neisseria strains were grown on Difco GCB agar containing Kellogg's supplement (Kellogg et al, 1968) at 37∞C, 5% CO 2 . , an epithelial-like human cell line from cervical carcinoma, was maintained in RPMI 1640 supplemented with 10% inactivated fetal bovine serum (FBS) and 2 mM Lglutamine at 37∞C, 5% CO 2 .…”

Section: Bacterial Strainsmentioning

confidence: 99%

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Epithelial cell responses induced upon adherence of pathogenic Neisseria

Plant¹,

Asp

Lövkvist³

et al. 2004

Cellular Microbiology

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SummaryNeisseria meningitidis and Neisseria gonorrhoeae colonize human mucosal surfaces and cause sepsis/ meningitis and gonorrhoea respectively. The first step in the infection process is pilus-mediated adhesion of the bacteria to epithelial cells, followed by host cell invasion. Adhesion of pathogenic Neisseria elicits multiple responses in host cells, including cellular signalling events, cytokine production and modulation of the eukaryotic cell surface. We used microarrays to assess the respective involvement of 375 human cytokine and adhesion related genes during adhesion of piliated and non-piliated N. gonorrhoeae , and piliated encapsulated N. meningitidis to the epithelial cell line ME-180. We identified 29 differentially regulated genes not previously reported to respond to neisserial infections, many of which encode membrane proteins. Selected genes were further analysed by semiquantitative RT-PCR, and protein expression was examined by flow cytometry. We found that N. gonorrhoeae elicited a different inflammatory response than N. meningitidis and we also demonstrated that early adhesion events are responsible for the induction of specific genes. Our data create a new platform for elucidating the interaction between pathogenic Neisseria and target cells.

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Section: Discussionsupporting

confidence: 79%

Section: Bacterial Strainsmentioning

confidence: 99%

Epithelial cell responses induced upon adherence of pathogenic Neisseria

Plant¹,

Asp

Lövkvist³

et al. 2004

Cellular Microbiology

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“…Non-pathogenic N. lactamica were obtained from Pharmacia and its properties have been described previously (Jonsson et al, 1991). The gonococcal strains were grown on GCB agar (Becton Dickinson) supplemented with Kellogg's complement (Kellogg et al, 1968) at 37∞C in 5% CO 2 and passaged every 18-20 h. Appropriate antibiotics were added when necessary. E. coli strain BL21 was grown on LB agar plates as described previously (Studier and Moffatt, 1986).…”

Section: Bacterial Strains and Growth Conditionsmentioning

confidence: 99%

Neisseria gonorrhoeae downregulates expression of the human antimicrobial peptide LL-37

Bergman

Johansson

Asp

et al. 2005

Cellular Microbiology

101

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SummaryNeisseria gonorrhoeae is a human pathogen causing the sexually transmitted disease gonorrhoeae. The bacteria preferentially attach to and invade epithelial cells of the genital tract. As these cells previously have been shown to express the human cathelicidin LL-37, we wanted to investigate the role of LL-37 during N. gonorrhoeae infection. The cervical epithelial cell line ME180 was utilized and the expression of LL-37 was confirmed on both peptide and transcriptional levels. Moreover, LL-37 exhibited potent in vitro activity against N. gonorrhoeae. Interestingly, the transcript and peptide levels of LL-37 were downregulated during infection, according to quantitative real-time polymerase chain reaction (PCR) and immunocyto-chemistry. The downregulation was most prominent with pathogenic strains of Neisseria , while non-pathogenic strains such as Neisseria lactamica and Escherichia coli only exhibited moderate effects. Heat-killed N. gonorrhoeae had no impact on the downregulation, emphasizing the importance of live bacteria. The results in this study suggest that pathogenic Neisseria may gain a survival advantage in the female genital tract by downregulating LL-37 expression.

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“…Tfp of pathogenic Neisseria is a macromolecular structure that mediates twitching motility (Wolfgang et al, 1998) and DNA uptake (Sparling, 1966). Early studies strongly suggest that Tfp is important for Ngo infection of humans (Kellogg et al, 1963;1968;Swanson et al, 1988). In vitro, Tfp initiates bacterial contact with epithelial cells and stimulates host cell signalling pathways that influence infection outcome (Nassif et al, 1994;Pujol et al, 1999;Helaine et al, 2005;Howie et al, 2005;Kirchner et al, 2005;Lee et al, 2005;Higashi et al, 2007;Exley et al, 2009;Boettcher et al, 2010;Swanson et al, 2011;Lecuyer et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

Sigma factor RpoN (σ⁵⁴) regulates pilE transcription in commensal Neisseria elongata

Rendón

Hockenberry

McManus

et al. 2013

Molecular Microbiology

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Summary Human-adapted Neisseria includes two pathogens, Neisseria gonorrhoeae and Neisseria meningitidis, and at least 13 species of commensals that colonize many of the same niches as the pathogens. The Type IV pilus plays an important role in the biology of pathogenic Neisseria. In these species, Sigma factor RpoD (σ70), Integration Host Factor, and repressors RegF and CrgA regulate transcription of pilE, the gene encoding the pilus structural subunit. The Type IV pilus is also a strictly conserved trait in commensal Neisseria. We present evidence that a different mechanism regulates pilE transcription in commensals. Using Neisseria elongata as a model, we show that Sigma factor RpoN (σ54), Integration Host Factor, and an activator we name Npa regulate pilE transcription. Taken in context with previous reports, our findings indicate pilE regulation switched from an RpoN- to an RpoD-dependent mechanism as pathogenic Neisseria diverged from commensals during evolution. Our findings have implications for the timing of Tfp expression and Tfp-mediated host cell interactions in these two groups of bacteria.

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Neisseria gonorrhoeae II. Colonial Variation and Pathogenicity During 35 Months In Vitro

Cited by 388 publications

References 12 publications

Epithelial cell responses induced upon adherence of pathogenic Neisseria

Epithelial cell responses induced upon adherence of pathogenic Neisseria

Neisseria gonorrhoeae downregulates expression of the human antimicrobial peptide LL-37

Sigma factor RpoN (σ⁵⁴) regulates pilE transcription in commensal Neisseria elongata

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Neisseria gonorrhoeae II. Colonial Variation and Pathogenicity During 35 Months In Vitro

Cited by 388 publications

References 12 publications

Epithelial cell responses induced upon adherence of pathogenic Neisseria

Epithelial cell responses induced upon adherence of pathogenic Neisseria

Neisseria gonorrhoeae downregulates expression of the human antimicrobial peptide LL-37

Sigma factor RpoN (σ54) regulates pilE transcription in commensal Neisseria elongata

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Sigma factor RpoN (σ⁵⁴) regulates pilE transcription in commensal Neisseria elongata