Nerium indicumleaf alleviates iron-induced oxidative stress and hepatic injury in mice

Abstract

Context: Nerium indicum Mill. (Apocynaceae) was reported for its efficient in vitro antioxidant and iron-chelating properties.Objective: This study demonstrates the effect of 70% methanol extract of N. indicum leaf (NIME) towards in vitro DNA protection and ameliorating iron-overload-induced liver damage in mice. Materials and methods: Phytochemical and HPLC analyses were carried out to standardize the extract and the effect of Fe 2+ -mediated pUC18 DNA cessation was studied. Thirty-six Swiss Albino mice were divided into six groups of blank, negative control (iron overload only), and iron-overloaded mice receiving 50, 100, and 200 mg/kg b.w. doses of NIME and desirox (20 mg/ kg b.w.). The biochemical markers of hepatic damage, various liver and serum parameters, and reductive release of ferritin iron were studied. Results and discussion: The presence of different phytocomponents was revealed from phytochemical and HPLC analyses. A substantial supercoiled DNA protection, with [P] 50 of 70.33 ± 0.32 mg, was observed. NIME (200 mg/kg b.w.) significantly normalized the levels of alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase, and bilirubin by 126.27, 125.25, 188.48, and 45.47%, respectively. NIME (200 mg/kg b.w.) was shown to alleviate the reduced levels of superoxide dismutase, catalase, glutathione-S-transferase, and nonenzymatic-reduced glutathione, by 48.95, 35.9, 35.42, and 13.22%, respectively. NIME also lowered raised levels of lipid peroxidation, protein carbonyl, hydroxyproline, and liver iron by 32.28, 64.58, 136.81, and 83.55%, respectively. Conclusion: These findings suggest that the active substances present in NIME may be capable of lessening iron overload-induced toxicity, and possibly be a useful drug for iron-overloaded diseases.

Haloalkane induced hepatic insult in murine model: amelioration by Oleander through antioxidant and anti-inflammatory activities, an in vitro and in vivo study

“…Our previous study showed potent iron chelation ability by the oleander extracts [11]. Recent studies also showed that 70 % hydro-methanolic extract of N. indicum leaf could ameliorate hemochromatosis and fibrosis in liver due to its potent antioxidant and iron chelation activities [13, 14]. Thus, the present study corroborates the previous findings of the antioxidative protection by oleander extracts.…”

“…The extracts also demonstrated free radical scavenging activities against various reactive species associated with hepatotoxicity such as hydroxyl (OH ● ), superoxide (O 2 ●− ), peroxynitrite (ONOO − ), singlet oxygen ( 1 O 2 ), hydrogen peroxide (H 2 O 2 ) and nitric oxide (NO), in addition to potent iron (Fe 2+ ) chelation activities, overload of which may lead to haemochromatic liver [12]. It is interesting to note that, a recent in vivo preliminary study demonstrated that N. indicum leaf extract ameliorates haemochromatic conditions in mouse [13]. Moreover, another recent study showed that N. indicum leaf extract attenuates CCl 4 mediated hepatotoxicity in Swiss albino mouse [14].…”

Long-Term Sodium Ferulate Supplementation Scavenges Oxygen Radicals and Reverses Liver Damage Induced by Iron Overloading

“…Our results indicated that both SF and TAU significantly reduced the aggravations in liver damage associated with iron overload. The effect of SF is explained by its antioxidation, which is consistent with previous studies that reported that antioxidant protects against liver damage in iron-overloaded mice models [20,21,22].…”

Atrial fibrillation in β‐thalassemia patients with a focus on the role of iron‐overload and oxidative stress: A review

“…One-third of the patients experienced improvement in mT2* and no new arrhythmias were detected during the 24 months (Aydinok et al, 2015). for example, silymarin (Moayedi Esfahani et al, 2015), phytic acid (Bhowmik et al, 2017), and 70% methanol extract of Nerium indicum leaf (NIME) (Ghate, Chaudhuri, Panja, & Mandal, 2015). Some of them showed less potency in terms of chelating iron when compared to several standard iron chelators (NIME vs DFX (Ghate et al, 2015)), and may not be suitable for iron chelation monotherapy.…”

“…The release of ferritin iron is essential for chelation. This process is facilitated by reducing agents such as ascorbate (Ghate et al, ). For this reason, some anti‐oxidants may be able to improve the efficacy of iron chelation.…”