<i>Nicotiana tabacum</i>protoplasts secretome can evidence relations among regulatory elements of exocytosis mechanisms

Ul-Rehman, Reiaz; Rinalducci, Sara; Zolla, Lello; Dalessandro, Giuseppe; Sansebastiano, Gian Pietro Di

doi:10.4161/psb.6.8.15750

Cited by 7 publications

(9 citation statements)

References 30 publications

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“…Although studies have described the role of SYP121 and SYP122 in the mechanics of vesicle fusion during exocytosis (Fasshauer et al, 1998;Bock et al, 2001;Bassham and Blatt, 2008;Karnik et al, 2015), the molecular mechanism of the selection of specific SNAREs at the plasma membrane and their contribution to the specificity of cargo proteins are not well known. Rehman et al (2008) and Ul- Rehman et al (2011) showed that SYP121 and SYP122 differ in their interaction with RABA2c (also known as RAB11A), resulting in differential secreted proteomes. Out of the nine identified tobacco proteins used by Ul- Rehman et al (2011) in their similarity assessment, our total data set included five possibly homologous proteins; however, these lacked signal peptides and were not included in our detailed analysis.…”

Section: Discussionmentioning

confidence: 99%

“…Rehman et al (2008) and Ul- Rehman et al (2011) showed that SYP121 and SYP122 differ in their interaction with RABA2c (also known as RAB11A), resulting in differential secreted proteomes. Out of the nine identified tobacco proteins used by Ul- Rehman et al (2011) in their similarity assessment, our total data set included five possibly homologous proteins; however, these lacked signal peptides and were not included in our detailed analysis. By analyzing the dominant sets of conventionally secreted proteins in the two mutants and in the wild type, we demonstrated that SYP121 and SYP122 differ in their contributions to the secretion of specific cargo proteins across the plasma membrane.…”

Section: Discussionmentioning

confidence: 99%

“…By contrast, SYP122 has been associated with cell wall deposition during early development (Assaad et al, 2004), bacterial pathogen-related defense (Nühse et al, 2003), and salicylic acid and jasmonic acid signaling (Assaad et al, 2004;Zhang et al, 2008). Rehman et al (2008) and Ul-Rehman et al (2011) have indicated potential differences between SYP121 and SYP122 based on heterologous associations with Rab GTPases in tobacco (Nicotiana tabacum). These studies show that SYP121 and SYP122 functions are clearly separable physiologically, but they offer few clues to the underlying basis for these differences.…”

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SNAREs SYP121 and SYP122 Mediate the Secretion of Distinct Cargo Subsets

et al. 2018

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A.M.E.J.).SNARE (soluble N-ethylmaleimide-sensitive factor attachment protein receptor) proteins drive vesicle fusion and contribute to homoeostasis, pathogen defense, cell expansion, and growth in plants. In Arabidopsis (Arabidopsis thaliana), two homologous Qa-SNAREs, SYNTAXIN OF PLANTS121 (SYP121) and SYP122, facilitate the majority of secretory traffic to the plasma membrane, and the single mutants are indistinguishable from wild-type plants in the absence of stress, implying a redundancy in their functions. Nonetheless, several studies suggest differences among the secretory cargo of these SNAREs. To address this issue, we conducted an analysis of the proteins secreted by cultured wildtype, syp121, and syp122 mutant Arabidopsis seedlings. Here, we report that a number of cargo proteins were associated differentially with traffic mediated by SYP121 and SYP122. The data also indicated important overlaps between the SNAREs. Therefore, we conclude that the two Qa-SNAREs mediate distinct but complementary secretory pathways during vegetative plant growth.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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SNAREs SYP121 and SYP122 Mediate the Secretion of Distinct Cargo Subsets

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“…Even if only a few proteins were identified, differences in the effects on exocytosis and protein transport were observed in an elegant experiment using transient over-expression of different SNAREs in tobacco protoplasts (Ul-Rehman et al, 2011)…”

Section: Biological Findingsmentioning

confidence: 99%

Plant secretome proteomics

Alexandersson¹,

Ali²,

Resjö³

et al. 2013

Front. Plant Sci.

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The plant secretome refers to the set of proteins secreted out of the plant cell into the surrounding extracellular space commonly referred to as the apoplast. Secreted proteins maintain cell structure and acts in signaling and are crucial for stress responses where they can interact with pathogen effectors and control the extracellular environment. Typically, secreted proteins contain an N-terminal signal peptide and are directed through the endoplasmic reticulum/Golgi pathway. However, in plants many proteins found in the secretome lack such a signature and might follow alternative ways of secretion. This review covers techniques to isolate plant secretomes and how to identify and quantify their constituent proteins. Furthermore, bioinformatical tools to predict secretion signals and define the putative secretome are presented. Findings from proteomic studies and important protein families of plant secretomes, such as proteases and hydrolases, are highlighted.

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“…One dimensional SDS-PAGE followed by LC-MS/MS is the most widely used technology to identify cell secretomes in various cell types of both plants and mammals [29, 30]. Novel proteins secreted by astrocytes were identified from conditioned media of murine astrocyte culture by a proteomics approach [31].…”

Section: Methodologies Available To Study Cell Secretomesmentioning

confidence: 99%

Methodologies to decipher the cell secretome

Mukherjee¹,

Mani²

2013

Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics

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The cell secretome is a collection of proteins consisting of transmembrane proteins (TM) and proteins secreted by cells into the extracellular space. A significant portion (~13-20%) of the human proteome consists of secretory proteins. The secretory proteins play important roles in cell migration, cell signaling and communication. There is a plethora of methodologies available like Serial Analysis of Gene Expression (SAGE), DNA microarrays, antibody arrays and bead-based arrays, mass spectrometry, RNA sequencing and yeast, bacterial and mammalian secretion traps to identify the cell secretomes. There are many advantages and disadvantages in using any of the above methods. This review aims to discuss the methodologies available along with their potential advantages and disadvantages to identify secretory proteins. This review is a part of a Special issue on The Secretome.

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Nicotiana tabacumprotoplasts secretome can evidence relations among regulatory elements of exocytosis mechanisms

Cited by 7 publications

References 30 publications

SNAREs SYP121 and SYP122 Mediate the Secretion of Distinct Cargo Subsets

SNAREs SYP121 and SYP122 Mediate the Secretion of Distinct Cargo Subsets

Plant secretome proteomics

Methodologies to decipher the cell secretome

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