<i>Nosema apis</i> infection in honey bee (<i>Apis mellifera</i>) queens

Webster, Thomas C.; Thacker, Etta M.; Pomper, Kirk W.; Lowe, Jeremiah D.; Hunt, Greg J.

doi:10.1080/00218839.2008.11101423

Cited by 11 publications

(9 citation statements)

References 15 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In Hungary, differentiation between N. ceranae and N. apis spores by PCR analysis reported a low incidence of N. apis in Hungarian apiaries (26). Webster et al (27) noted that PCR analysis was more sensitive than examination for spores by light microscopy in detecting N. apis infection. Different researchers who used the same technique determined N. ceranae for the first time in European honeybee colonies in Canada and the central USA (28), France (20), Spain (29), Jordan (30), and the United States (31).…”

Section: Discussionmentioning

confidence: 99%

Molecular detection of Nosema spp. and black queen-cell virusin honeybees in Van Province, Turkey

Oğuz¹,

Karapınar²,

Dinçer³

et al. 2017

Turk J Vet Anim Sci

View full text Add to dashboard Cite

This study was planned to determine the prevalences of the Nosema spp. and the black queen-cell virus (BQCV) among honeybees (Apis mellifera) raised in the province of Van by PCR and to determine the molecular characteristics of the determined isolates. A total of 260 adult worker bees from 26 colonies at 5 apiary locations belonging to the province of Van in April and May 2015 were collected for this reason. Samples were examined microscopically. In the case of positivity, spore identification was done by multiplex PCR. Reverse transcription/PCR analysis (RT/PCR) was carried out for the BQCV analysis. At the end of the microscopic examination, Nosema spp. spores were detected in 8 out of 26 colonies (32.5%). The result of multiplex-PCR revealed Nosema ceranae positivity in all of the samples, but no Nosema apis was determined. As a result of the RT/PCR tests of the samples BQCV was detected in 23 (88.5%) of the total 26 colonies. This study is the first to investigate Nosema spp. and BQCV with the PCR technique in bees raised in the province of Van.

show abstract

Section: Discussionmentioning

confidence: 99%

Molecular detection of Nosema spp. and black queen-cell virusin honeybees in Van Province, Turkey

Oğuz¹,

Karapınar²,

Dinçer³

et al. 2017

Turk J Vet Anim Sci

View full text Add to dashboard Cite

show abstract

“…An emerging gut parasite, Nosema ceranae, and the more familiar N. apis, are of particular interest because studies have shown that these microsporidians can be transferred to the queen via trophallaxis of contaminated food during shipment in queen mailing cages (Webster et al, 2008;Williams et al, 2008). The absence of N. apis and N. ceranae, as well as the tracheal mite Acarapis woodi, among the sampled queens in the current study is encouraging, particularly in light of previous studies showing very high infection rates (Furgala, 1962;Jay and Dixon, 1984;Camazine et al, 1998), and it suggests that commercial queen producers utilize effective management practices regarding the prevention and spread of these parasites.…”

Section: Discussionmentioning

confidence: 99%

“…While honey bees are hosts to a wide variety of parasites and pathogens (Schmid-Hempel, 1998), only a subset tend to infect queen bees. The more notable parasites of queens are tracheal mites Acarapis woodi (Burgett and Kitprasert, 1992;Camazine et al, 1998;Villa and Danka, 2005), the gut protozoan Nosema apis (Webster et al, 2004(Webster et al, , 2008 and, presumably, N. ceranae (Higes et al, 2006(Higes et al, , 2008. Moreover, queens may be infected with numerous viruses (Chen et al, 2005;Yang and Cox-Foster, 2005), including acute bee paralysis virus (ABPV), chronic bee paralysis virus (CBPV), black queen cell virus (BQCV), deformed wing virus (DWV), Kashmir bee virus (KBV), sacbrood virus (SBV), and Israeli acute paralysis virus (IAPV).…”

Section: Introductionmentioning

confidence: 99%

The physical, insemination, and reproductive quality of honey bee queens (Apis mellifera L.)

et al. 2011

View full text Add to dashboard Cite

-Understanding the reproductive potential ("quality") of queens bees can provide valuable insights into factors that influence colony phenotype. We assayed queens from various commercial sources for various measures of potential queen quality, including their physical characters (such as their degree of parasitism), insemination number (stored sperm counts), and effective paternity frequency (number of drone fathers among their offspring). We found significant variation in the physical, insemination, and mating quality of commercially produced queens, and we detected significant correlations within and among these various measures. Overall, the queens were sufficiently inseminated (3.99 ± 1.504 million sperm) and mated with an appropriate number of drones (effective paternity frequency: 16.0 ± 9.48). Importantly, very few of the queens were parasitized by tracheal mites and none were found with either Nosema species. These findings suggest possible mechanisms for assessing the potential fitness of honey bee queens without the need for destructive sampling.honey bee queens / reproductive potential / insemination / parasitism / effective mating frequency

show abstract

“…As with workers, a range of different doses have been used to study effects of infection on queens individually fed, although again, more spores were provided than were required (Alaux et al 2010c;Webster, 2008). Using individual feeding, queens and worker bees have become infected using similar spore doses (Webster et al, 2004), but the infectious doses for queens have never been established for either of the two microsporidian infections discussed.…”

Section: Study Of Dose Effectsmentioning

confidence: 99%

Standard methods forNosemaresearch

Fries

Chauzat²,

Chen³

et al. 2013

Journal of Apicultural Research

329

302

View full text Add to dashboard Cite

SummaryMethods are described for working with Nosema apis and Nosema ceranae in the field and in the laboratory. For fieldwork, different sampling methods are described to determine colony level infections at a given point in time, but also for following the temporal infection dynamics.Suggestions are made for how to standardise field trials for evaluating treatments and disease impact. The laboratory methods described include different means for determining colony level and individual bee infection levels and methods for species determination, including light microscopy, electron microscopy, and molecular methods (PCR). Suggestions are made for how to standardise cage trials, and different inoculation methods for infecting bees are described, including control methods for spore viability. A cell culture system for in vitro rearing of Nosema spp. is described. Finally, how to conduct different types of experiments are described, including infectious dose, dose effects, course of infection and longevity tests. Métodos estándar para la investigación sobre Nosema ResumenSe describen procedimientos para trabajar con Nosema apis y Nosema ceranae en el campo y en el laboratorio. Para el trabajo de campo, se describen diferentes métodos de muestreo para determinar infecciones al nivel de colonia en un momento determinado, y también para el seguimiento de la dinámica temporal de infección. Se hacen sugerencias para la forma de estandarizar los ensayos de campo para evaluar los tratamientos y el impacto de la enfermedad. Los métodos de laboratorio descritos incluyen diferentes formas de determinar los niveles de infección al nivel de colonia y de abeja individual, y los métodos para la determinación de las especies, incluyendo microscopía óptica, microscopía electrónica y métodos moleculares (PCR). Se hacen sugerencias para estandarizar los ensayos con cajas, y se describen diferentes métodos de inoculación para infectar abejas, incluyendo métodos de control para la viabilidad de las esporas. Se describe un sistema de cultivo celular para la cría in vitro de Nosema spp. Finalmente, se describe cómo llevar a cabo diferentes tipos de experimentos, incluyendo la dosis infecciosa, efectos de la dosis, curso de la infección y las pruebas de longevidad.

show abstract

Nosema apis infection in honey bee (Apis mellifera) queens

Cited by 11 publications

References 15 publications

Molecular detection of Nosema spp. and black queen-cell virusin honeybees in Van Province, Turkey

Molecular detection of Nosema spp. and black queen-cell virusin honeybees in Van Province, Turkey

The physical, insemination, and reproductive quality of honey bee queens (Apis mellifera L.)

Standard methods forNosemaresearch

Contact Info

Product

Resources

About