<i>NR6A1</i>Allelic Frequencies as an Index for both Miniaturizing and Increasing Pig Body Size

Ijiri, Moe; Lai, Yu‐Chang; Kawaguchi, Hiroaki; Fujimoto, Yoshikazu; Miura, Naoki; Matsuo, Tomohide; Tanimoto, Akihide

doi:10.21873/invivo.12244

Cited by 5 publications

(5 citation statements)

References 26 publications

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“…The conclusion in our study is that the function of these variations is complex (positive or negative). Moe et al [ 10 ] found NR6A1 gene heterozygous genotype (TC) of c.575T > C were observed in the middle-sized indigenous pig (LTN = 21), while homozygous (TT) were fixed in the European commercial breed (Large White, LTN = 22), and homozygous CC were fixed in the Micromini pig (LTN = 19). This loci (c.575T > C) was not found in our study, possibly as the donkey, as an ancient species, did not undergo strong artificial selection.…”

Section: Discussionmentioning

confidence: 99%

“…Body length is an important factor affecting livestock slaughter performance [ 7 ], as a longer carcass means longer loins and more meat production, a larger body size means a larger body surface area and more skin yield. A variation in vertebrae number is found in a few domestic animals such as pigs [ 8 , 9 , 10 ], sheep [ 11 , 12 , 13 ], and cattle [ 14 ]. In European commercial pig breeds, a 14% variation in body length can be attributed to vertebrae number variation, for each additional vertebrae, there was an increase in length of about 15 mm [ 15 ].…”

Section: Introductionmentioning

confidence: 99%

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Multi-Thoracolumbar Variations and NR6A1 Gene Polymorphisms Potentially Associated with Body Size and Carcass Traits of Dezhou Donkey

Liu

Gao

Wang

et al. 2022

Animals

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The number of thoracolumbar vertebrae is a quantitative trait positively correlated with the economic traits of livestock. More thoracolumbar vertebrae individuals could genetically be used to improve the livestock population, as more thoracolumbar vertebrae means a longer carcass, which could bring more meat production. Nuclear receptor subfamily 6 group A member 1 (NR6A1) is considered a strong candidate gene for effecting the number of vertebrae in livestock. The purposes of this study are as follows: (a) Analyzing the effect of TLN variation on body size and carcass traits of Dezhou donkey; (b) Studying the distribution of seven single nucleotide variants (SNVs) in NR6A1 gene of Dezhou donkey; (c) Exploring the relationship between latent SNVs and TLN, the body size and carcass traits. We examined the thoracic and lumbar vertebrae number and seven SNVs in NR6A1 gene of 455 Dezhou donkeys, and analyzed the relationships between them. Five types of thoracolumbar combinations (T17L5 (individual with 17 thoracic and five lumbar vertebrae) 2.4%, T18L5 75.8%, T19L5 1.1%, T17L6 11.9%, and T18L6 8.8%) of Dezhou donkeys were detected in this study. For one thoracolumbar vertebra added, the body length of Dezhou donkey increases by 3 cm and the carcass weight increases by 6 kg. Seven SNVs (g.18093100G > T, g.18094587G > T, g.18106043G > T, g.18108764G > T, g.18110615T > G, g.18112000C > T and g.18114954T > G) of the NR6A1 gene were found to have a significant association with the TLN, body size and carcass traits of Dezhou donkey (p < 0.05), respectively. For instance, g.18114954C > T is significantly associated with lumber vertebrae number, the total number of thoracolumbar, and carcass weight, and individuals with TT genotype had significantly larger value than CC genotype (p < 0.05). Using these 7SNVs, 16 different haplotypes were estimated. Compared to Hap3Hap3, individuals homozygous for Hap2Hap2 showed significantly longer length in one thoracic spine (STL), the total thoracic vertebrae and one thoracolumbar spine. Our study will not only extend the understanding of genetic variation in the NR6A1 gene of Dezhou donkey, but also provide useful information for marker assisted selection in donkey breeding program.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Multi-Thoracolumbar Variations and NR6A1 Gene Polymorphisms Potentially Associated with Body Size and Carcass Traits of Dezhou Donkey

Liu

Gao

Wang

et al. 2022

Animals

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“…Cell proliferation and apoptosis are inextricably linked to the number of cells [ 37 ]. Studies have shown that systemic factors (e.g., growth hormone) and local signaling molecules (e.g., IGF ) control the proliferation and apoptosis of various cells in the body, ultimately controlling body size [ 23 , 38 ].…”

Section: Discussionmentioning

confidence: 99%

“…Although studies that used candidate gene (CG) markers to explore the genetic basis of body shape in miniature pigs are extensive [ 23 , 24 , 25 ], these markers are not yet fully understood at present. Accordingly, the current study aimed to compare the genetic divergence between miniature and large pigs worldwide to recognize the genetic mechanism behind miniature pigs with published SNP data from swine genome sequencing data.…”

Section: Introductionmentioning

confidence: 99%

Identification of Body Size Determination Related Candidate Genes in Domestic Pig Using Genome-Wide Selection Signal Analysis

Pan

Long

Yuan

et al. 2022

Animals

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This study aimed to identify the genes related to the body size of pigs by conducting genome-wide selection analysis (GWSA). We performed a GWSA scan on 50 pigs belonging to four small-bodied pig populations (Diannan small-eared pig, Bama Xiang pig, Wuzhishan pig, and Jeju black pig from South Korea) and 124 large-bodied pigs. We used the genetic parameters of the pairwise fixation index (FST) and π ratio (case/control) to screen candidate genome regions and genes related to body size. The results revealed 47,339,509 high-quality SNPs obtained from 174 individuals, while 280 interacting candidate regions were obtained from the top 1% signal windows of both parameters, along with 187 genes (e.g., ADCK4, AMDHD2, ASPN, ASS1, and ATP6V0C). The results of the candidate gene (CG) annotation showed that a series of CGs (e.g., MSTN, LTBP4, PDPK1, PKMYT1, ASS1, and STAT6) was enriched into the gene ontology terms. Moreover, molecular pathways, such as the PI3K-Akt, HIF-1, and AMPK signaling pathways, were verified to be related to body development. Overall, we identified a series of key genes that may be closely related to the body size of pigs, further elucidating the heredity basis of body shape determination in pigs and providing a theoretical reference for molecular breeding.

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“…In this case, cellular and tissue regenerative confirmation is difficult and tends to be given less consideration due to difficulties in tracing the transplanted cells. Consequently, we focused on micro-mini pigs (MMP) for their convenient body size [ 2 ] and experimental design [ 3 , 4 , 5 ]. Similarly, GFP-Tg Jinhua pigs were developed as an imaging source for in vivo experiments [ 6 ], and GFP-Tg MMP were further developed using the back-crossing method [ 7 ].…”

Section: Introductionmentioning

confidence: 99%

Differentiation Capacity of Porcine Skeletal Muscle-Derived Stem Cells as Intermediate Species between Mice and Humans

Tamaki

Natsume

Katoh

et al. 2023

IJMS

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Large animal experiments are important for preclinical studies of regenerative stem cell transplantation therapy. Therefore, we investigated the differentiation capacity of pig skeletal muscle-derived stem cells (Sk-MSCs) as an intermediate model between mice and humans for nerve muscle regenerative therapy. Enzymatically extracted cells were obtained from green-fluorescence transgenic micro-mini pigs (GFP-Tg MMP) and sorted as CD34+/45− (Sk-34) and CD34−/45−/29+ (Sk-DN) fractions. The ability to differentiate into skeletal muscle, peripheral nerve, and vascular cell lineages was examined via in vitro cell culture and in vivo cell transplantation into the damaged tibialis anterior muscle and sciatic nerves of nude mice and rats. Protein and mRNA levels were analyzed using RT-PCR, immunohistochemistry, and immunoelectron microscopy. The myogenic potential, which was tested by Pax7 and MyoD expression and the formation of muscle fibers, was higher in Sk-DN cells than in Sk-34 cells but remained weak in the latter. In contrast, the capacity to differentiate into peripheral nerve and vascular cell lineages was significantly stronger in Sk-34 cells. In particular, Sk-DN cells did not engraft to the damaged nerve, whereas Sk-34 cells showed active engraftment and differentiation into perineurial/endoneurial cells, endothelial cells, and vascular smooth muscle cells, similar to the human case, as previously reported. Therefore, we concluded that Sk-34 and Sk-DN cells in pigs are closer to those in humans than to those in mice.

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NR6A1Allelic Frequencies as an Index for both Miniaturizing and Increasing Pig Body Size

Cited by 5 publications

References 26 publications

Multi-Thoracolumbar Variations and NR6A1 Gene Polymorphisms Potentially Associated with Body Size and Carcass Traits of Dezhou Donkey

Multi-Thoracolumbar Variations and NR6A1 Gene Polymorphisms Potentially Associated with Body Size and Carcass Traits of Dezhou Donkey

Identification of Body Size Determination Related Candidate Genes in Domestic Pig Using Genome-Wide Selection Signal Analysis

Differentiation Capacity of Porcine Skeletal Muscle-Derived Stem Cells as Intermediate Species between Mice and Humans

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