<i>Nysius cymoides</i> (Hemiptera: Lygaeidae), a New Economically Important Pest on <i>Acacia tortilis</i> and Its Intracellular Bacterial Endosymbionts

Koohpayma, Fatemeh; Bagheri, Abdoolnabi; Fallahzadeh, Majid; Dousti, Abu Fazel; Askari-Seyahooei, Majeed

doi:10.3157/021.128.0103

Cited by 4 publications

(5 citation statements)

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“…Based on the phylogenetic placement and the lack of other published records of Sulcia from Heteroptera, we strongly suspect that the detection of Sulcia in Nysius cymoides reported by Koohpayma et al. (2018) resulted from sample contamination. Further studies of non‐auchenorrhynchan Hemiptera are needed to confirm the absence of Sulcia or Sulcia‐like endosymbionts in these groups.…”

Section: Discussionmentioning

confidence: 82%

“…The role of Sulcia as a primary endosymbiont of Auchenorrhyncha is well established. Other than Auchenorrhyncha, only one moss bug and one true bug were previously reported as hosts of Sulcia (Müller, 1962; Koohpayma et al., 2018), but both of these records are questionable. Prior to the availability of molecular methods for characterizing bacteria, Müller (1962) grouped the endosymbiont found in a species of Peloridiidae with the most common symbiont of Auchenorrhyncha (“a‐symbiont”, later identified as Sulcia) based on morphological similarity.…”

Section: Discussionmentioning

confidence: 99%

“…Phylogenetic analysis placed this sequence in the clade of four Deltocephalinae leafhopper-associated strains with high support, although the sister relationship between the strains from Nysius cymoides and Orosius albicinctus was not strongly supported. Based on the phylogenetic placement and the lack of other published records of Sulcia from Heteroptera, we strongly suspect that the detection of Sulcia in Nysius cymoides reported by Koohpayma et al (2018) resulted from sample contamination. Further studies of nonauchenorrhynchan Hemiptera are needed to confirm the absence of Sulcia or Sulcia-like endosymbionts in these groups.…”

Section: Association Between Sulcia and Hemipteran Insectsmentioning

confidence: 93%

“…Our screening of the transcriptomes of four species of Peloridiidae failed to detect the presence of Sulcia. Koohpayma et al (2018) reported Sulcia from the true bug Nysius cymoides based on a 16S sequence highly similar (99% identity, but without reporting matching length or depositing the sequence in a public database) to the 16S rRNA of a Sulcia strain from the leafhopper Orosius albicinctus. Phylogenetic analysis placed this sequence in the clade of four Deltocephalinae leafhopper-associated strains with high support, although the sister relationship between the strains from Nysius cymoides and Orosius albicinctus was not strongly supported.…”

Section: Association Between Sulcia and Hemipteran Insectsmentioning

confidence: 99%

“…Sulcia belongs to Phylum Bacteroidetes and is closely related to Flavobacteriales (Moran et al., 2005; McCutcheon and Moran, 2007; Matsuura et al., 2018). So far, Sulcia is only definitely recorded from the hemipteran suborder Auchenorrhyncha, with doubtful reports from a moss bug (Coleorrhyncha) and a true bug (Heteroptera) (Müller, 1962; Koohpayma et al., 2018). During the long‐term association with auchenorrhynchan insects, the Sulcia genome has undergone remarkable reduction (< 0.3 Mb), but retained conserved functions for nutritional synthesis (Wu et al., 2006; McCutcheon et al., 2009).…”

Section: Introductionmentioning

confidence: 99%

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Phylogenomics of flavobacterial insect nutritional endosymbionts with implications for Auchenorrhyncha phylogeny

Cao

Dietrich

2021

Cladistics

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Candidatus Sulcia muelleri" (Sulcia) is a diverse lineage of intracellular nutritional endosymbiotic bacteria strictly associated with auchenorrhynchous hemipteran insects. Sulcia has undergone long-term codiversification with its insect hosts but the phylogeny of these endosymbionts, their relationships to other bacteria, and the extent of their occurrence within various groups of Auchenorrhyncha remain inadequately explored. Comprehensive phylogenetic analyses of Sulcia and related bacteria were performed to elucidate its position relative to other members of Phylum Bacteroidetes and the degree of congruence to the phylogeny of its auchenorrhynchous hosts. Maximum likelihood (ML) and maximum parsimony (MP) analyses of Flavobacteriales based on genomic data from 182 bacterial strains recover a monophyletic Sulcia within a larger clade of flavobacterial insect endosymbionts, closely related to Weeksellaceae. Molecular divergence time analysis of Sulcia dates the origin of Sulcia at approximately 339.95 million years ago (Myr) and the initial divergence within Sulcia at approximately 256.91 Myr but these are considered underestimates due to the tendency for endosymbionts to evolve at higher rates compared to their free-living relatives. Screening of 96 recently sequenced hemipteran transcriptomes revealed that 73 of these species, all Auchenorrhyncha, harbored Sulcia. Phylogenetic analysis of 131 orthologous genes plus 16S rRNA for 101 Sulcia strains, representing six fulgoroid families and all the families of Cicadomorpha except Tettigarctidae, recover largely congruent phylogenies between Sulcia and Auchenorrhyncha. The phylogeny of Sulcia strongly supports the superfamily relationships Fulgoroidea + (Cicadoidea + (Cercopoidea + Membracoidea)). Relationships within individual superfamilies are also largely concordant, with the few areas of apparent incongruence between Sulcia and insect genes attributable to low branch support in one or both datasets. These results suggest that analysis of Sulcia phylogeny may contribute to resolution of contentious aspects of Auchenorrhyncha phylogeny.

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Section: Discussionmentioning

confidence: 82%