<i>O</i>-Sulfation disposition of curcumin and quercetin in SULT1A3 overexpressing HEK293 cells: the role of arylsulfatase B in cellular <i>O</i>-sulfation regulated by transporters

Pei, Shu-Hua; Dou, Yuanyuan; Zhang, Wenke; Qi, Defei; Li, Yingying; Wang, Mengqing; Li, Wenqi; Shi, Hongxiang; Gao, Zixuan; Yao, Chaoyan; Fang, Dong; Sun, Hua; Xie, Songqiang

doi:10.1039/d2fo01436j

Cited by 4 publications

(1 citation statement)

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“…SULT1A3 is an important member of SULT1 family and has received particular interest, due to their abilities to catalyze the sulfonation of numerous phenolic substrates (Hui et al, 2015; James & Ambadapadi, 2013; Pei et al, 2022). SULT1A3 has been reported to be responsible for the majority of neurotransmitter sulfonation in the central nervous system, sulfonating more than 90% of all dopamine and ∼80% of serotonin in the extracellular brain fluid (Cook et al, 2021; Itaaho et al, 2007).…”

Section: Introductionmentioning

confidence: 99%

Farnesoid X receptor represses human sulfotransferase 1A3 expression through direct binding to the promoter

Dou

Pei

et al. 2023

Chem Biol Drug Des

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Human sulfotransferases 1A3 (SULT1A3) has received particular interest, due to their functions of catalyzing the sulfonation of numerous phenolic substrates, including bioactive endogenous molecules and therapeutic agents. However, the regulation of SULT1A3 expression and the underlying mechanism remain unclear. Here, we aimed to investigate the regulation effects of bile acid‐activated farnesoid X receptor (FXR) on SULT1A3 expression, and to shed light on the mechanism thereof. Our results demonstrated that FXR agonists (CDCA and GW4064) significantly inhibit the expression of SULT1A3 at mRNA and protein levels. In addition, overexpression of FXR led to decrease in SULT1A3 expression and knockdown of FXR significantly induced the expression of SULT1A3 in protein and mRNA levels, confirming that FXR expression manifestly showed negative regulatory effect on basal SULT1A3 expression. Furthermore, a combination of luciferase reporter gene and CHIP assays showed that FXR repressed SULT1A3 transcription through direct binding to the region at base pair positions −664 to −654. In conclusion, this study for the first time confirmed FXR was a negative transcriptional regulator of human SULT1A3 enzyme.

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Section: Introductionmentioning

confidence: 99%

Farnesoid X receptor represses human sulfotransferase 1A3 expression through direct binding to the promoter

Dou

Pei

et al. 2023

Chem Biol Drug Des

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Unraveling the chemical constituents, absorption characteristics, and metabolic profile of Codonopsis Radix based on UPLC-Q- Orbitrap MS

Pei,

Wang,

Wang

et al. 2024

Journal of Pharmaceutical and Biomedical Analysis

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A critical examination of human data for the biological activity of quercetin and its phase-2 conjugates

Williamson,

Clifford

2024

Critical Reviews in Food Science and Nutrition

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O-Sulfation disposition of curcumin and quercetin in SULT1A3 overexpressing HEK293 cells: the role of arylsulfatase B in cellular O-sulfation regulated by transporters

Abstract: Extensive phase II metabolic reactions (i.e. gulcuronidation and sulfonation) have resulted in low bioavailability and decreased biological effects of curcumin and quercetin. Compared to glucuronidation, information on the sulfonation disposition...

Cited by 4 publications

References 58 publications

Farnesoid X receptor represses human sulfotransferase 1A3 expression through direct binding to the promoter

Farnesoid X receptor represses human sulfotransferase 1A3 expression through direct binding to the promoter

Unraveling the chemical constituents, absorption characteristics, and metabolic profile of Codonopsis Radix based on UPLC-Q- Orbitrap MS

A critical examination of human data for the biological activity of quercetin and its phase-2 conjugates

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