<i>Oenococcus oeni</i>
            Genome Plasticity Is Associated with Fitness

Bon, Elisabeth; Delaherche, Arnaud; Bilhere, Eric; Daruvar, Antoine de; Lonvaud‐Funel, Aline; Marrec, Claire Le

doi:10.1128/aem.02194-08

Cited by 57 publications

(51 citation statements)

References 52 publications

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“…CadC binds specifically to the cad operator DNA and is released by the addition of Cd 2+ , Pb 2+ , and Bi 3+ (Endo and Silver 1995). Genetic analyses in L. lactis and Oenococcus oeni have shown the occurrence of similar plasmid-encoded cadmium-resistance systems in LAB (Bon et al 2009;Liu et al 1997). Additionally, cadmium may be pumped out of the cell by multidrug transporters.…”

Section: Response To Cadmiummentioning

confidence: 97%

Responses of Lactic Acid Bacteria to Heavy Metal Stress

Solioz

Mermod

Abicht

et al. 2011

Stress Responses of Lactic Acid Bacteria

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Section: Response To Cadmiummentioning

confidence: 97%

Responses of Lactic Acid Bacteria to Heavy Metal Stress

Solioz

Mermod

Abicht

et al. 2011

Stress Responses of Lactic Acid Bacteria

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“…In any case, the specific molecular analysis applied in this work showed a higher percentage of O. oeni lysogeny (67.6%) than any previously published data based on prophage induction by external agents (7,32,33). Nevertheless, it should be noted that some phage remnants have been found in the pangenome of bacteria (28,41), and further studies should be conducted on the strains that possess lys genes but are not inducible to properly characterize phages.…”

Section: Discussionmentioning

confidence: 49%

Development of a New Method for Detection and Identification of Oenococcus oeni Bacteriophages Based on Endolysin Gene Sequence and Randomly Amplified Polymorphic DNA

Doria

Napoli

Costantini

et al. 2013

Appl Environ Microbiol

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M alolactic fermentation (MLF) is a secondary fermentation that occurs in wine after the alcoholic fermentation. This fermentation can take place spontaneously through the action of the population of lactic acid bacteria (LAB) present in the wine or by the use of commercial starters, mainly belonging to the species Oenococcus oeni, the principal LAB species responsible for MLF in wine (1, 2).As in other fermented foods, like dairy products, in which bacteriophages represent a potential risk for the fermentative process (3, 4), bacteriophages have also been reported to be a possible cause of unsuccessful MLF in wine (5, 6). Therefore, preparation of commercial starters that take into account the different sensitivities of O. oeni strains to different phages has been proposed (5, 7). However, under laboratory conditions, O. oeni has a very low growth rate, and its poorly visible colonies are hardly produced in agar medium, hampering the accurate detection of O. oeni phages, which has contributed to the low number of studies conducted until now on this topic. For instance, while PCR-based approaches to detect bacteriophages have been used in bacteria of the genera Lactobacillus and Lactococcus present in dairy products (8-11), such methods have never been applied to detect phages infecting O. oeni. In the present study, a new PCR method for the detection of bacteriophages of O. oeni without the need for a sensitive indicator strain and for a growth step on solid medium to confirm the presence of prophages was developed.Until now, typing of O. oeni bacteriophages has been conducted by morphological characterization, structural-protein composition, host range analysis, and/or restriction enzyme analysis (5, 12-15). The last approach (15) was the first DNA-based technique that allowed the grouping of different O. oeni phage types.Bacteriophages of O. oeni present in wines were first observed by Sozzi et al. (12) by electron microscopy (EM) and were subsequently described as being different in size (5). Later, EM analyses of four O. oeni phages showed that they were very similar, exhibiting a Bradley type B morphology, with the presence of a head, a tail, and a base plate (13). Similar results were reported after EM analysis of 10 phages isolated from wines (14), which also showed similar morphology with slight differences in the head size, tail length, and presence of a base plate. These phages were divided into four groups based on structural-protein composition, host range, and restriction enzyme analysis. Likewise, comparable morphologies and structural protein profiles have also been reported in 17 O. oeni mitomycin C (mit C)-induced phages from different O. oeni strains, which were further divided into six groups based on their enzymatic restriction patterns (15).Endolysins, also termed lysins, are enzymes encoded by most double-stranded DNA phage genomes that lyse bacterial cell walls and cause host death (16,17). Detection of the lys gene has recently been used to evaluate phages infecting Lactobacillus helve-

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“…Oenococcus oeni seems indeed to be endowed with a plastic genome (Bon et al 2009;Borneman et al 2010) that is presumably so because it lacks the MisMatch Repairing system (Marcobal et al 2008). Strain-specific regions, possibly linked to the organisms' ability to adapt to its original niche, have been identified ).…”

Section: Analysis Of Negative and Positive Oenological Traitsmentioning

confidence: 96%

Selection criteria and tools for malolactic starters development: an update

2010

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The use of malolactic starter cultures to improve the fermentation process and enhance wine quality and safety is becoming a common winemaking practice, increasingly preferred to spontaneous fermentation. Given its great oenological properties, Oenococcus oeni is the species most present in commercial brands of wine lactic acid bacteria (LAB) starters. Stress resistance, technological performances and safety are the key selection criteria to take into account when designing effective malolactic starters. Nowadays, new LAB strains are selected by exploiting advanced technological applications rather than the traditional screening methods based on a trial and error approach. In particular, the progress made in the fields of genetics and molecular biology, as well as in the whole genome sequencing projects, offers new tools to better characterize candidate starter strains. This review aims at providing an updated picture of the technological approaches that should be used to select LAB strains suitable for winemaking. In the near future, the full integration of phenotypic and genetic data will make it possible to rationally develop malolactic cultures that are specific for different types of wine.

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Oenococcus oeni Genome Plasticity Is Associated with Fitness

Cited by 57 publications

References 52 publications

Responses of Lactic Acid Bacteria to Heavy Metal Stress

Responses of Lactic Acid Bacteria to Heavy Metal Stress

Development of a New Method for Detection and Identification of Oenococcus oeni Bacteriophages Based on Endolysin Gene Sequence and Randomly Amplified Polymorphic DNA

Selection criteria and tools for malolactic starters development: an update

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