PABPC1 Enables Cells with the Suspension Cultivation Feature

Abstract

Cell-based vaccine manufacturing is an important strategy for viral disease prevention. Cultivating cells in suspension could maximize the utility of large bioreactors for cost-effective and scaled up vaccine production, where adapting adherent cells to suspension culture is the bottleneck and key. Through whole transcriptome sequencing of suspension and adherent strains of BHK-21 and CHO-K1 cells followed by the identification of differentially expressed genes, mutational analysis, gene ontology, and pathway enrichment analysis, we identified four candidate genes, PABPC1, LARS, GLUL, PFN1, feasible for genetically modulating anchorage-dependent cells toward cell suspension culture, and experimentally validated the functionality of PABPC1 in both BHK-21 and CHO-K1 cells. Our study unveiled a novel role of PABPC1 that could potentially aid in the establishment of a cost-effective vaccine manufacturing platform relying on cell cultivation in suspension.

Cytoplasmic poly(A)-binding protein 1 as a biomarker to assist early diagnosis and prognosis of esophageal squamous cell carcinoma in endoscopic biopsy fragments

“…Cytoplasmic poly(A)-binding protein 1 (PABPC1) is the main cytoplasmic isoform of poly(A) binding proteins that function as an essential part in mRNA stabilization and translation enhancement (Kini et al , 2016; Dai et al , 2021). PABPC1 is important in regulating most cellular processes, resulting in a close relationship between tumor genesis and malignant development.…”