<i>Paecilomyces variotii</i> central nervous system infection in a patient with cancer

Kantarcıoğlu, A. Serda; Hatemi, G.; Yücel, Ayhan; Hoog, G. S. de; Mandel, Nil Molınas

doi:10.1046/j.1439-0507.2003.00837.x

Cited by 18 publications

(6 citation statements)

References 31 publications

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“…32 P. variotii is the second species in this category, and it was strongly amplified by the specificity testing performed here. Besides acting as an opportunistic pathogen, 33 P. variotii has been listed as a species commonly found in indoor environments, 34 and in moisture-damaged schools. 35 A phylogenetic tree of the 18S gene indicates the species is very genetically similar to Aspergillus.…”

Section: Discussionmentioning

confidence: 99%

Real-time PCR for detection of the Aspergillus genus

et al. 2007

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Aspergillus is a genus of mold that has strong indoor sources, including several species capable of acting as opportunistic pathogens. Previous studies suggest that Aspergillus could serve as an indicator for abnormal mold growth or moisture, making it an important genus for environmental monitoring. Here, a quantitative polymerase chain reaction (qPCR, or real-time PCR) assay is presented for Aspergillus. The assay shows good specificity for the genus, detecting all Aspergillus species tested, although a few non-Aspergillus species are also amplified. Sensitivity testing demonstrates that DNA representing one conidium can be detected. A validation study compared qPCR results against direct microscopy counts using A. fumigatus conidia aerosolized into a laboratory chamber. The assay was then used to quantify Aspergillus in indoor air samples, demonstrating its utility for environmental monitoring. Analysis of a small number of clinical sputum samples showed complete agreement with culturing results.

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Section: Discussionmentioning

confidence: 99%

Real-time PCR for detection of the Aspergillus genus

et al. 2007

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“…Other pathogenic ascomycetes with a marked predilection towards brain infections, such as Pseudallescheria boydii , Candida albicans , and Aspergillus fumigatus (de Hoog & Guarro, 2000), have also been isolated from environments containing hydrocarbons or phenols, and are known to assimilate these substrates (Sorkhoh et al , 1990; Jones et al , 1995; Gaylarde et al , 1999). The common fuel biodeteriogen Paecilomyces variotii also occurs as an opportunistic pathogen, occasionally causing central nervous system infections in immunocompromised patients (de Hoog & Guarro, 2000; Kantarcioglu et al , 2003). Infections with Sporothrix schenckii (a species mentioned above as closely related to an alkylbenzene‐degrading isolate surveyed here) are usually localized in the subcutaneous and lymphatic tissues, but disseminated infections with neurotropic involvement have occasionally been observed in individuals with impaired immunity (Rocha et al , 2001).…”

Section: Ecology Of the Alkylbenzene‐utilizing Fungimentioning

confidence: 99%

Fungi growing on aromatic hydrocarbons: biotechnology's unexpected encounter with biohazard?

Prenafeta-Boldú¹,

Summerbell²,

Hoog³

2006

FEMS Microbiol Rev

245

175

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The biodegradation of aromatic hydrocarbons by fungi has traditionally been considered to be of a cometabolic nature. Recently, however, an increasing number of fungi isolated from air biofilters exposed to hydrocarbon-polluted gas streams have been shown to assimilate volatile aromatic hydrocarbons as the sole source of carbon and energy. The biosystematics, ecology, and metabolism of such fungi are reviewed here, based in part on re-evaluation of a collection of published hydrocarbon-degrading isolates obtained from authors around the world. Incorrect or outdated identifications in original publications are corrected by ribosomal DNA sequence analysis. The data show that many volatile-hydrocarbon-degrading strains are closely related to, or in some cases clearly conspecific with, the very restricted number of human-pathogenic fungal species causing severe mycoses, especially neurological infections, in immunocompetent individuals. Neurochemistry features a distinctive array of phenolic and aliphatic compounds that are related to molecules involved in the metabolism of aromatic hydrocarbons. Hence, there may be physiological connections between hydrocarbon assimilation and certain patterns of mammalian infection.

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“…Many fungi from these genera either are opportunistic pathogens (e.g. Penicillium chrysogenum, P. brevicompactum, Paecilomyces lilacinus, P. variotii, A. niger, A. fumigatus ), [8,24–28] or can produce mycotoxins that are carcinogenic to humans (e.g. P. commune, A. ochraceus, A. flavus ) [2,29,30].…”

Section: Resultsmentioning

confidence: 99%

Extensive set of mitochondrial LSU rDNA-based oligonucleotide probes for the detection of common airborne fungi

Zeng

Rasmuson-Lestander

Wang

2004

FEMS Microbiology Letters

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Fungi exist in every indoor and outdoor environment. Many fungi are toxigenic or pathogens that may cause various public health concerns. Rapid and accurate detection and identification of fungi require specific markers. In this study, partial mitochondrial large subunit rDNA was amplified and sequenced from 32 fungal strains representing 31 species from 14 genera. Based on the sequence variation pattern, 26 oligonucleotide probes were designed for their discrimination. The specificity of the probes was evaluated through homology search against GenBank database and hybridization examination on 38 fungal strains. The 26 probes were verified as highly specific to 20 fungal species. A two-step detection procedure through PCR followed by probe hybridization gave ten-fold increase in detection sensitivity than single-step PCR assay and would be a practical approach for environmental sample screening. The probes developed in this study can be applied in clinical diagnosis and environmental monitoring of fungal agents.

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Paecilomyces variotii central nervous system infection in a patient with cancer

Cited by 18 publications

References 31 publications

Real-time PCR for detection of the Aspergillus genus

Real-time PCR for detection of the Aspergillus genus

Fungi growing on aromatic hydrocarbons: biotechnology's unexpected encounter with biohazard?

Extensive set of mitochondrial LSU rDNA-based oligonucleotide probes for the detection of common airborne fungi

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