<i>Paenibacillus macerans</i>Possesses Two Types of 16S rDNA Copies in a Genome with a Length Difference of Twelve Base Pairs

Hamasaki, Yoshikatsu; Watanabe, Yoshihisa; Kotoura, Satoshi; Fuchu, Hidetaka; Sugiyama, Masaaki; Hashizume, Katsuhito

doi:10.1271/bbb.69.1995

Cited by 5 publications

(3 citation statements)

References 12 publications

(13 reference statements)

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“…The cloned 16S rRNA genes revealed three types of sequences in strains B27 T and F6-B70, indicating intra-genomic heterogeneity, which has been found in other species of the genus Paenibacillus (Bosshard et al , 2002; Hamasaki et al , 2005; Nübel et al , 1996; Roux & Raoult, 2004). Comparative 16S rRNA gene sequence analyses showed that strains B27 T and F6-B70 were phylogenetically affiliated to the genus Paenibacillus .…”

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confidence: 71%

Paenibacillus tianmuensis sp. nov., isolated from soil

Fang

Qian

et al. 2011

International Journal of Systematic and Evolutionary Microbiology

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confidence: 71%

Paenibacillus tianmuensis sp. nov., isolated from soil

Fang

Qian

et al. 2011

International Journal of Systematic and Evolutionary Microbiology

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“…32 Most of these bacteria are found in soil and on plants and are known or presumed to form endospores. 12,16,20,21,26,40,49 In one contamination, we also identified M. luteus, which belongs to the phylum Actinobacteria and is an obligate aerobe associated with soil, plants, and the human skin microbiome. 17 The characteristics of the contaminants do not directly allow reconstruction of the contamination routes, but they suggest that contamination occurred either via surfaces contaminated by contact with human skin (for example, hands) or unclean air or through the introduction of contaminated feed or bedding.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of Peroxides and Chlorine Oxides as Disinfectants for Chemical Sterilization of Gnotobiotic Rodent Isolators

Moody

Miyamoto

Ang

et al. 2019

j am assoc lab anim sci

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Gnotobiotic animal research has expanded markedly over the past decade. Although germ-free animals were first described more than 100 y ago, little evidence-based guidance is available on best operational procedures. A key aspect of gnotobiotic technology is the sterilization of animal enclosures, most commonly flexible vinyl film isolators. The objective of this study was to determine the most effective methods for chemical sterilization of gnotobiotic isolators and associated equipment. As test microbes, we used bacteria from 4 different accidental isolator contaminations that occurred in a gnotobiotic core facility. Identification by 16S ribotyping revealed facultative anaerobic firmicutes, including several Paenibacillus and Bacillus species, and obligate aerobic actinobacteria, namely Micrococcus luteus, among the contaminants. We selected 6 products commonly used for disinfecting hospital rooms, kitchens, and veterinary facilities to represent chlorine-oxideand peroxide-based disinfectants and tested the hypothesis that these 2 classes are equally effective. However, evaluation of bactericidal and sporicidal activity in liquid cultures revealed that chlorine oxide-based disinfectants were more effective than peroxide-based disinfectants. In both groups, various products effectively sterilized gnotobiotic isolators by fogging in field tests, although bactericidal concentrations were markedly higher than those in suspension cultures, and effectiveness was contact-time-dependent. In addition, in both groups, some disinfectants were excessively corrosive to ferrous metals and acrylic. These results demonstrate that no single disinfectant has all desirable properties and that the different characteristics of disinfectants must be balanced during their selection. However, chlorine oxide-based disinfectants were generally more effective and less corrosive than peroxide-based products.

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“…two universal primers each with 0.2 mM concentration, 2.5 U Tay DNA polymerase (Genei, Bangalore), and four deoxynucleoside triphosphate each in 0.2 mM concentration. The primer sequence selection was done from the regions of 16s rDNA and the 16s rDNA was amplified by using universal forward primer 5-TGGAGAGTTTGATCCTGGCTCAG-3' and universal reverse primer 5'-TACCGCGGCTGCTGGCAC-3' (Hamasaki et al, 2005;Hall et al, 2003). After an initial denaturation for 2 min at 95°C, 25 cycles were completed each consisting of Imin at 94C, 50 sec at 60°C annealing temperature, and 1.5min at 72°C.…”

Section: Pcr Amplificationmentioning

confidence: 99%

Bacterial strain isolated from the Mango field and identified on the basis of 16S rDNA sequence information and optimization of the alkaline cellulase production

Roy¹,

Chattopadhyay²

2008

NBUJPS

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A bacterial strain was isolated from the soil of mango (Mangifera indica) orchards. The isolate was identified as Paenibacillus ahvei [MTCC 7809] through phylogenetic analysis based on the 16S rDNA gene sequencing and also by conventional biochemical processes. The scanning electron microscopic analysis revealed the morphological details of the strain. The isolate is Gram positive. motile, rod shaped, spore forming bacterium with extreme salt tolerance. It can hydrolyze starch and gelatin, and catalase and oxidase were produced. The cellulase activity of the culture broth was determined by measuring the reducing sugar released from carboxymethyl cellulose (CMC). Different cultural conditions were tested to reach the optimum growth conditions in order to obtain large-scale production of alkaline cellulase for commercial importance. Crude cellulase from Paenibacillus alvei (MTCC 7809) showed activity and stability at moderately high temperature and pH and the activity remained stable at 40°C and pH 8 after 2h of incubation making the strain a good choice for industrial applications. The rDNA sequence of 1509bp is submitted into the NCBI GenBank database (Acession no. EF429201).

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Paenibacillus maceransPossesses Two Types of 16S rDNA Copies in a Genome with a Length Difference of Twelve Base Pairs

Cited by 5 publications

References 12 publications

Paenibacillus tianmuensis sp. nov., isolated from soil

Paenibacillus tianmuensis sp. nov., isolated from soil

Evaluation of Peroxides and Chlorine Oxides as Disinfectants for Chemical Sterilization of Gnotobiotic Rodent Isolators

Bacterial strain isolated from the Mango field and identified on the basis of 16S rDNA sequence information and optimization of the alkaline cellulase production

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